公开(公告)号：EP4443137A1

公开(公告)日：2024-10-09

申请号：EP23461553.2

申请日：2023-04-07

申请人： Uniwersytet Jagiellonski ,  Uniwersytet Medyczny w Lodzi

发明人： Majzner, Katarzyna ,  Adamczyk, Adriana ,  Nowakowska, Anna ,  Baranska, Malgorzata ,  Jakubowska, Justyna ,  Mlynarski, Wojciech

IPC分类号： G01N21/65 ,  G01N33/50 ,  G01N33/543

CPC分类号： G01N21/65 ,  G01N33/54373 ,  G01N33/5047 ,  G01N33/5073

摘要： The invention relates to a new method of identifying differentiation into neutrophils of human promyelocytic leukaemia HL-60 cell line, which is an in vitro model of hematopoietic cells, which involves calculating the ratio of the band intensities characteristic of cytochrome, lipids and MitoBADY reporter, which signal, together with the integrated intensities of lipids and cytochrome bands present in the Raman spectra of living cells, allows for distinguishing between differentiated and undifferentiated cells. The method used in the embodiments to measure the intensity ratio of these bands is Raman spectroscopy.

公开(公告)号：EP4354142A1

公开(公告)日：2024-04-17

申请号：EP22461622.7

申请日：2022-10-15

申请人： UNIWERSYTET JAGIELLONSKI ,  Uniwersytet Medyczny w Lodzi

发明人： Majzner, Katarzyna ,  Leszczenko, Patrycja ,  Nowakowska, Anna ,  Bara ska, Ma gorzata ,  Jakubowska, Justyna ,  Z bczy ska, Marta ,  Pastorczak, Agata ,  Ostrowska, Kinga ,  M ynarski, Wojciech

IPC分类号： G01N33/574

CPC分类号： G01N33/57426

摘要： A method of identifying the ALL molecular subtype with rearrangements within the KMT2A gene was disclosed, which consists in measuring the ratio of the intensity of the Raman 1040/1008 cm-1 Raman bands in leukemia cells (lymphoblasts) isolated from the peripheral blood (when blastosis >90%) or bone marrow of patients during the diagnosis of acute lymphoblastic leukemia, and fixed with an aldehyde reagent.