公开(公告)号：EP1825259B1

公开(公告)日：2009-11-18

申请号：EP05810922.4

申请日：2005-11-29

申请人： VIB vzw ,  Universiteit Gent

发明人： CHEN, Cuiying ,  CONTRERAS, Roland

IPC分类号： G01N33/50

CPC分类号： G01N33/50 ,  G01N2333/4722 ,  G01N2400/12 ,  Y10T436/143333

公开(公告)号：EP2171463A1

公开(公告)日：2010-04-07

申请号：EP08760873.3

申请日：2008-06-11

申请人： VIB VZW ,  Universiteit Gent

发明人： CHEN, Cuiying ,  CONTRERAS, Roland

IPC分类号： G01N33/574 ,  C12Q1/68

CPC分类号： G01N33/57438 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N2400/14 ,  Y10T436/143333

摘要： The invention provides methods and kits to detect early stage hepatocellular carcinoma or a change in the gradation of hepatocellular carcinoma in mammals. The diagnostic marker is based on the profiling and identification of diagnostic carbohydrates present in a body fluid such as blood serum.

公开(公告)号：EP1583968A2

公开(公告)日：2005-10-12

申请号：EP04701959.1

申请日：2004-01-14

申请人： VIB vzw ,  UNIVERSITEIT GENT

发明人： CONTRERAS, Roland ,  CALLEWAERT, Nico

IPC分类号： G01N33/66

CPC分类号： G01N33/6893 ,  G01N2800/085 ,  G01N2800/52 ,  Y10T436/142222 ,  Y10T436/143333

摘要： Abstract The invention provides methods and kits to detect liver fibrosis or a change in the gradation of liver fibrosis in mammals. The diagnostic marker is based on the profiling and identification of diagnostic carbohydrates present in a body fluid such as blood serum.

公开(公告)号：EP1583968B1

公开(公告)日：2015-04-22

申请号：EP04701959.1

申请日：2004-01-14

申请人： VIB vzw ,  Universiteit Gent

发明人： CONTRERAS, Roland ,  CALLEWAERT, Nico

IPC分类号： G01N33/66

CPC分类号： G01N33/6893 ,  G01N2800/085 ,  G01N2800/52 ,  Y10T436/142222 ,  Y10T436/143333

公开(公告)号：EP1601761A1

公开(公告)日：2005-12-07

申请号：EP04718970.9

申请日：2004-03-10

申请人： VIB vzw ,  UNIVERSITEIT GENT

发明人： CONTRERAS, Roland ,  GEYSENS, Steven

IPC分类号： C12N1/15 ,  C12N1/19 ,  C12N9/24 ,  C12P21/00

CPC分类号： C12Y302/01113 ,  C12N9/2434 ,  C12P21/00 ,  C12Y204/0115 ,  C12Y302/0102 ,  C12Y302/01084

摘要： The present invention relates to the use of a glucosidase II mutation to increase protein secretion in eukaryotic cells. The present invention relates further to the use of an eukaryotic cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant α-1,2 mannosidase gene and/or a recombinant N-acetylglucosaminyl-transferase gone, as a host for protein secretion.

公开(公告)号：EP1825259A1

公开(公告)日：2007-08-29

申请号：EP05810922.4

申请日：2005-11-29

申请人： VIB vzw ,  Universiteit Gent

发明人： CHEN, Cuiying ,  CONTRERAS, Roland

IPC分类号： G01N33/50

CPC分类号： G01N33/50 ,  G01N2333/4722 ,  G01N2400/12 ,  Y10T436/143333

摘要： The present invention relates to a marker that can be used as aging biomarker. More specifically, the present invention relates to the analysis of N-glycans in serum and its relation to the virtual age of the subject. This aging biomarker can be used to study the effect of medication, food compounds and/or special diets on the wellness and virtual age of animals, including humans.

摘要翻译： 本发明涉及可用作衰老生物标志物的标志物。 更具体地说，本发明涉及血清中N-聚糖的分析及其与受试者虚拟年龄的关系。 这种老化的生物标志物可用于研究药物，食物化合物和/或特殊饮食对动物（包括人类）的健康和虚拟年龄的影响。

公开(公告)号：EP1294910B1

公开(公告)日：2008-11-19

申请号：EP01955499.7

申请日：2001-06-29

申请人： VIB vzw ,  Research Corporation Technologies, Inc

发明人： CONTRERAS, Roland ,  CALLEWAERT, Nico, L., M. ,  GEYSENS, Steven, C., J.

IPC分类号： C12N15/80 ,  C12N15/62 ,  C12N15/56 ,  C12N9/24 ,  C12P21/00

CPC分类号： C12P21/005 ,  A61K38/00 ,  C12N9/2402 ,  C12N9/2488 ,  C12N15/81 ,  C12N15/815 ,  C12R1/84 ,  C12Y302/0102 ,  C12Y302/01024 ,  Y02P20/52

摘要： The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an α-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoprotiens with reduced glycosylation using such genetically engineered stains of Pichia are also provided.