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    METHOD FOR IDENTIFICATION AND DETECTION OF MUTANT GENE USING INTERCALATOR
    2.
    发明公开
    METHOD FOR IDENTIFICATION AND DETECTION OF MUTANT GENE USING INTERCALATOR 有权
    方法鉴别和识别变异基因WITH A嵌入

    公开(公告)号：EP2840137A4

    公开(公告)日：2016-01-06

    申请号：EP13764366

    申请日：2013-03-22

    申请人： WAKO PURE CHEM IND LTD ,  KANAGAWA PREFECTURAL HOSPITAL ORGANIZATION

    发明人： MATSUKUMA SHOICHI ,  ISHIKAWA TOMOKAZU ,  KUROSAWA TATSUO

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6823 ,  C12Q1/6827 ,  G01N2458/30 ,  C12Q2563/173 ,  C12Q2565/125 ,  C12Q2565/131 ,  C12Q2565/133

    摘要： The present invention addresses the problem of improving separation performance in separating a hybrid of a mutant-type DNA and a probe or/and a hybrid of a wild-type type DNA and a probe, said hybrids being obtained by the LH method. The present invention relates to "a method for detection of mutant-type DNA or/and wild-type DNA which comprises the following steps: (1) a step in which at least one of a single-stranded DNA having a substituted nucleotide, a deficient nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA corresponding to the mutant-type DNA (wild-type DNA) is contacted with a probe hybridizing with both single-stranded DNAs, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with the wild-type DNA (wild-type hybrid) (in this regard however, at least one of the obtained mutant-type hybrid and wild-type hybrid has a loop structure), (2) a step in which the obtained mutant-type hybrid or/and wild-type hybrid is contacted with an intercalator, and (3) a step in which the presence or absence of the mutant-type DNA or/and the wild-type DNA is detected by separating the conjugate of mutant-type hybrid and intercalator or/and the conjugate of wild-type hybrid and intercalator".

    METHOD FOR DETECTING MUTANT DNA
    3.
    发明公开
    METHOD FOR DETECTING MUTANT DNA 有权
    方法用于检测DNA突变

    公开(公告)号：EP2620509A4

    公开(公告)日：2014-03-12

    申请号：EP11826926

    申请日：2011-09-22

    申请人： WAKO PURE CHEM IND LTD ,  KANAGAWA PREFECTURAL HOSPITAL ORGANIZATION

    发明人： MATSUKUMA SHOICHI ,  ISHIKAWA TOMOKAZU

    IPC分类号： C12Q1/68 ,  C12N15/09 ,  G01N27/447 ,  G01N33/53

    CPC分类号： C12Q1/6827 ,  C12Q2565/125 ,  C12Q2565/131

    摘要： The present invention relates to a method for detecting of a mutant DNA using a probe, comprising: (1) contacting a sample containing a single-stranded DNA which has a substituted nucleotide, a deleted nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA (wild-type DNA) corresponding thereto with the probe which hybridizes with both single-stranded DNA, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with a wild-type DNA (wild-type hybrid), wherein at least one of the obtained mutant-type hybrid and wild-type hybrid has the stem structure; (2) separating the obtained mutant-type hybrid or/and wild-type hybrid by electrophoresis on the basis of presence or absence of the stem structure or difference in the stem structure; and (3) detecting the presence or absence of the mutant-type DNA in the sample.

    PRIMER AND PROBE FOR USE IN DETECTION OF MYCOBACTERIUM KANSASII AND METHOD FOR DETECTION OF MYCOBACTERIUM KANSASII USING THE SAME
    5.
    发明公开
    PRIMER AND PROBE FOR USE IN DETECTION OF MYCOBACTERIUM KANSASII AND METHOD FOR DETECTION OF MYCOBACTERIUM KANSASII USING THE SAME 有权
    FOR USE引物和探针BE MIKOBACTERIUM kansaii道伊方法的检测FOR分枝杆菌检测kansaii如此

    公开(公告)号：EP1881068A4

    公开(公告)日：2009-07-15

    申请号：EP06746311

    申请日：2006-05-11

    申请人： WAKO PURE CHEM IND LTD

    发明人： ISHIKAWA TOMOKAZU

    IPC分类号： C12N15/09 ,  C12Q1/04 ,  C12Q1/68

    CPC分类号： C12Q1/04 ,  C12Q1/689 ,  G01N33/56922 ,  G01N2333/35

    摘要： An oligonucleotide which comprises a part or the entire of the nucleotide sequence depicted in SEQ ID NO:1, 2, 3 or 4 or a part or the entire of a sequence complementary to the nucleotide sequence and is capable of hybridizing with the nucleotide sequence for Mycobacterium kansasii gene; a primer and a probe for use in the detection of Mycobacterium kansasii comprising the oligonucleotide; and a method for detecting Mycobacterium kansasii using the primer and/or probe. The method for detecting Mycobacterium kansasii enables the detection of M. kansasii more rapidly and with higher accuracy compared with a conventional bacterium identification method performed by culture examination on a bacterium. Further, the method can exclude any false positive result for the diagnosis and can also detect and diagnose M. kansasii with higher accuracy compared with a diagnosis method performed by PCR using a conventional primer and/or probe. Still further, the method can quantify a M. kansasii cell.

    PROBE AND PRIMER FOR TUBERCLE BACILLUS DETECTION, AND METHOD OF DETECTING HUMAN TUBERCLE BACILLUS THEREWITH
    7.
    发明公开
    PROBE AND PRIMER FOR TUBERCLE BACILLUS DETECTION, AND METHOD OF DETECTING HUMAN TUBERCLE BACILLUS THEREWITH 有权
    探针和引物FOR结核杆菌用于检测人，这样检测和方法

    公开(公告)号：EP1746156A4

    公开(公告)日：2008-08-20

    申请号：EP05734679

    申请日：2005-04-22

    申请人： WAKO PURE CHEM IND LTD

    发明人： ISHIKAWA TOMOKAZU

    IPC分类号： C12Q1/68 ,  C12N15/09 ,  G01N33/53 ,  G01N33/569

    CPC分类号： C12Q1/689 ,  C12Q2600/16 ,  Y10S435/975

    摘要： Novel probe and primer for tubercle bacillus detection capable of avoiding diagnostic false positive; and a simple and easy, rapid, high-precision method of detecting human tubercle bacillus with the use thereof. There is provided an oligonucleotide comprising a part or the entirety of a nucleic acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8 or comprising a part or the entirety of a sequence complementary thereto, which oligonucleotide is capable of hybridization with the nucleic acid sequence of IS6110 gene of human tubercle bacillus. Further, there are provided a probe and primer for tubercle bacillus detection, comprising the oligonucleotide. Still further, there is provided a method of detecting human tubercle bacillus, characterized in that use is made of the above probe and primer.