公开(公告)号：EP1692258A4

公开(公告)日：2007-03-21

申请号：EP04801001

申请日：2004-11-12

申请人： XU XIAO ,  ABBASSI YAMA ,  WANG XIAOBO ,  GAN JIANGBO

发明人： XU XIAO ,  ABBASSI YAMA ,  WANG XIAOBO ,  GAN JIANGBO

IPC分类号： C12M1/34 ,  C12M1/42 ,  C12N20060101 ,  G01N27/02 ,  G01N27/07 ,  G01N33/487 ,  H01M4/50

CPC分类号： G01N33/6854 ,  C12M25/08 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/5005 ,  G01N2500/10

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.

公开(公告)号：EP1749203A4

公开(公告)日：2009-05-06

申请号：EP05722991

申请日：2005-02-09

申请人： XU XIAO ,  ABBASSI YAMA ,  WANG XIAOBO

发明人： XU XIAO ,  ABBASSI YAMA ,  WANG XIAOBO

IPC分类号： G01N27/02 ,  C12M1/34 ,  G01N33/487 ,  G01N33/543

CPC分类号： G01N33/4836 ,  C12M25/08 ,  C12M41/36 ,  G01N27/021 ,  G01N33/5005

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.