公开(公告)号：EP1749203A4

公开(公告)日：2009-05-06

申请号：EP05722991

申请日：2005-02-09

申请人： XU XIAO ,  ABBASSI YAMA ,  WANG XIAOBO

发明人： XU XIAO ,  ABBASSI YAMA ,  WANG XIAOBO

IPC分类号： G01N27/02 ,  C12M1/34 ,  G01N33/487 ,  G01N33/543

CPC分类号： G01N33/4836 ,  C12M25/08 ,  C12M41/36 ,  G01N27/021 ,  G01N33/5005

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.

公开(公告)号：EP1749203A2

公开(公告)日：2007-02-07

申请号：EP05722991.6

申请日：2005-02-09

申请人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo

发明人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo

IPC分类号： G01N27/02 ,  G01N33/487 ,  G01N33/543 ,  C12M1/34

CPC分类号： G01N33/4836 ,  C12M25/08 ,  C12M41/36 ,  G01N27/021 ,  G01N33/5005

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.

公开(公告)号：EP1692258A4

公开(公告)日：2007-03-21

申请号：EP04801001

申请日：2004-11-12

申请人： XU XIAO ,  ABBASSI YAMA ,  WANG XIAOBO ,  GAN JIANGBO

发明人： XU XIAO ,  ABBASSI YAMA ,  WANG XIAOBO ,  GAN JIANGBO

IPC分类号： C12M1/34 ,  C12M1/42 ,  C12N20060101 ,  G01N27/02 ,  G01N27/07 ,  G01N33/487 ,  H01M4/50

CPC分类号： G01N33/6854 ,  C12M25/08 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/5005 ,  G01N2500/10

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.

公开(公告)号：EP2639576A1

公开(公告)日：2013-09-18

申请号：EP13171137.6

申请日：2005-02-09

申请人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo

发明人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo

IPC分类号： G01N27/02 ,  G01N33/487 ,  G01N33/543 ,  C12M1/34

CPC分类号： C12M25/08 ,  C12M41/36 ,  G01N27/021 ,  G01N33/4836 ,  G01N33/5005

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.

公开(公告)号：EP2450698A1

公开(公告)日：2012-05-09

申请号：EP11193882.5

申请日：2005-02-09

申请人： Abbassi, Yama ,  Wang, Xiaobo ,  Xu, Xiao

发明人： Abbassi, Yama ,  Wang, Xiaobo ,  Xu, Xiao

IPC分类号： G01N27/02 ,  G01N33/487 ,  G01N33/543 ,  C12M1/34

CPC分类号： C12M25/08 ,  C12M41/36 ,  G01N27/021 ,  G01N33/4836 ,  G01N33/5005

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. The methods can be used to test the effects of compounds on cells, such as in cytotoxicity assays. Methods of cytotoxicity profiling of compounds are also provided.

摘要翻译： 本发明包括装置，系统，以及用于使用细胞底物阻抗监测测定细胞的方法。 在一个方面，本发明提供了细胞 - 基底阻抗监测装置上的不导电基板，其中每个阵列具有跨越整个阵列在大致均匀的电极电阻的确包括电极阵列。 在另一个方面，本发明提供了细胞 - 基底的监测系统，其包括一种或多种细胞底物监测装置包括以阻抗分析仪电极阵列，每个具有多个孔，一个设备站没有连接各个孔的阵列以阻抗分析仪和软件 用于控制设备站和阻抗分析器。 在另一个方面，本发明提供细胞试验确实使用了阻抗监测，以检测在细胞行为或状态的变化。 的方法可用于测试化合物对细胞的作用，如在细胞毒性试验检测。 因此，提供的化合物的细胞毒性分析的方法。

公开(公告)号：EP1692258A2

公开(公告)日：2006-08-23

申请号：EP04801001.1

申请日：2004-11-12

申请人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo ,  Gan, Jiangbo

发明人： Xu, Xiao ,  Abbassi, Yama ,  Wang, Xiaobo ,  Gan, Jiangbo

IPC分类号： C12M1/34 ,  C12M1/42

CPC分类号： G01N33/6854 ,  C12M25/08 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/5005 ,  G01N2500/10

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.