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    METHOD FOR PREPARING SOIL MICROORGANISM AND UTILIZATION OF SAME
    4.
    发明公开
    METHOD FOR PREPARING SOIL MICROORGANISM AND UTILIZATION OF SAME 审中-公开
    土壤微生物制剂的制备方法及其应用

    公开(公告)号：EP2835421A1

    公开(公告)日：2015-02-11

    申请号：EP13760804.8

    申请日：2013-03-12

    申请人： Japan Agency for Marine-Earth Science and Technology

    发明人： KOYAMA, Sumihiro ,  TSUBOUCHI, Taishi

    IPC分类号： C12N1/00 ,  C12N11/00 ,  C12N15/09 ,  C12Q1/04 ,  C12Q1/68 ,  C12M1/42

    CPC分类号： C12Q1/6806 ,  C12M25/08 ,  C12N1/20 ,  C12N13/00 ,  C12Q1/02

    摘要： An object of the present invention is to prepare individual microorganisms or a microflora composition derived from land or seafloor soil in which contamination of land or seafloor soil-derived substances is reduced. The present invention provides [1] a method for preparing, from a land or a seafloor soil samples, microorganisms containing a reduced level of land or seafloor soil-derived substances that inhibit nucleic acid analysis, which comprises (1) the step of disposing a suspension of a land or seafloor soil sample containing microorganisms and land or seafloor soil-derived substance that inhibit nucleic acid analysis as an electrolyte on a surface of a substrate at least a part of which constitutes an electrode, and applying a constant potential to the electrode to attach at least a part of the microorganisms to the surface of the substrate, and (2) the step of applying a high-frequency wave potential to the electrode to detach the microorganisms attached to the surface of the substrate in a viable state, and wherein the constant potential applied in the step (1) is greater than -0.5 V but not greater than-0.2 V (vs. Ag/AgCl), or greater than +0.2 V but not greater than +0.4 V (vs. Ag/AgCl), and the high-frequency wave potential applied in the step (2) has a frequency in the range of from 1 kHz to 20 MHz, and a potential range of ±0.1 V (vs. Ag/AgCl) or smaller.

    摘要翻译： 本发明的一个目的是制备单独的微生物或源自陆地或海底土壤的微生物群落组成，其中陆地或海底土壤来源物质的污染减少。 本发明提供[1]一种从陆地或海底土壤样品制备含有减少的抑制核酸分析的陆地或海底土壤衍生物质的微生物的方法，其包括（1） 将含有微生物的陆地或海底土壤样品和抑制核酸分析的陆地或海底土壤衍生物质作为电解质悬浮在至少一部分构成电极的基底的表面上，并将恒定电势施加到电极 将微生物的至少一部分附着于基板表面的工序;（2）向电极施加高频电位，使附着于基板表面的微生物以活性状态分离的工序， 其中步骤（1）中施加的恒定电位大于-0.5V但不大于-0.2V（vs.Ag/AgCl），或大于+ 0.2V但不大于+0.4V（相对于Ag / AgCl电极）， 并且在步骤（2）中施加的高频波电位具有在1kHz至20MHz的范围内的频率，并且±0.1V的电位范围（vs. Ag / AgCl）或更小。

    REAL TIME ELECTRONIC CELL SENSING SYSTEMS AND APPLICATIONS FOR CELL-BASED ASSAYS
    5.
    发明公开
    REAL TIME ELECTRONIC CELL SENSING SYSTEMS AND APPLICATIONS FOR CELL-BASED ASSAYS 审中-公开
    电子实时细胞知觉系统和应用程序做检查ON CELL BASE

    公开(公告)号：EP1692258A4

    公开(公告)日：2007-03-21

    申请号：EP04801001

    申请日：2004-11-12

    申请人： XU XIAO ,  ABBASSI YAMA ,  WANG XIAOBO ,  GAN JIANGBO

    发明人： XU XIAO ,  ABBASSI YAMA ,  WANG XIAOBO ,  GAN JIANGBO

    IPC分类号： C12M1/34 ,  C12M1/42 ,  C12N20060101 ,  G01N27/02 ,  G01N27/07 ,  G01N33/487 ,  H01M4/50

    CPC分类号： G01N33/6854 ,  C12M25/08 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/5005 ,  G01N2500/10

    摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.

    METHOD FOR PREPARATION OF PROLIFERATABLE ANIMAL CELLS
    9.
    发明公开
    METHOD FOR PREPARATION OF PROLIFERATABLE ANIMAL CELLS 审中-公开
    VERFAHREN ZUR HERSTELLUNG PROLIFERIERBARER TIERZELLEN

    公开(公告)号：EP2495309A1

    公开(公告)日：2012-09-05

    申请号：EP10826847.5

    申请日：2010-10-29

    申请人： Japan Agency for Marine-Earth Science and Technology

    发明人： KOYAMA, Sumihiro

    IPC分类号： C12N5/071 ,  A61L27/00 ,  C12M3/00 ,  C12N5/07

    CPC分类号： C12N5/00 ,  C12M25/08 ,  C12M33/00 ,  C12M35/02 ,  C12N2529/00 ,  C12N2539/10

    摘要： A method for preparing animal cells with good adhesion and proliferation properties which are capable of proliferation is provided. the method permits the detachment of cultured cells without damaging the cells. A method for preparing a sheet of animal cells such as skin cells which are capable of proliferation is also provided. A method for preparing animal cells capable of proliferation, comprising the steps of (1) culturing animal cells on a substrate at least one portion of which is an electrode, and (2) applying a high-frequency wave potential to the electrode to detach the cells that have adhered to the substrate surface through culturing. The high-frequency wave potential is of a frequency falling within a range of 1 KHz to 10 MHz with a potential falling within a range of ± 1.0 V (vs. Ag/AgCl) or less. The culture medium during separation does not contain calcium or magnesium.

    摘要翻译： 提供了一种制备具有良好粘附性和增殖性能的能够增殖的动物细胞的方法。 该方法允许培养的细胞脱离而不破坏细胞。 还提供了一种制备能够增殖的皮肤细胞的动物细胞片的方法。 一种制备能够增殖的动物细胞的方法，包括以下步骤：（1）在基材上培养动物细胞，其中至少一部分是电极，和（2）向所述电极施加高频波电位以分离 通过培养粘附于基材表面的细胞。 高频波电位为1KHz〜10MHz的范围，电位落在±1.0V（相对于Ag / AgCl）以下的范围内。 分离过程中的培养基不含钙或镁。

    INSTRUMENT AND METHOD FOR TREATING MINOR AMOUNT OF BIOLOGICAL MATERIAL
    10.
    发明公开
    INSTRUMENT AND METHOD FOR TREATING MINOR AMOUNT OF BIOLOGICAL MATERIAL 审中-公开
    仪器在VERFAHREN ZUR BEHANDLUNG EINER KLEINEREN MENGE AN BIOLOGISCHEM MATERIAL

    公开(公告)号：EP2241614A1

    公开(公告)日：2010-10-20

    申请号：EP09700562.3

    申请日：2009-01-09

    申请人： The University of Tokyo

    发明人： TAKEUCHI, Shoji ,  ONOE, Hiroaki

    IPC分类号： C12M3/00 ,  C12M1/14 ,  C12M1/40 ,  C12Q1/00 ,  C12N5/06

    CPC分类号： C12M25/08 ,  B01L3/502761 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2200/0668 ,  B01L2300/0819

    摘要： Provided is a technique for handling a minor amount of a biological material with the purpose of observing selectively the interaction between single cells by controlling cell number and two- or three-dimensional configurations of each of the different cell types. The present invention provides an instrument and a method for handling a minor amount of a biological material. The instrument of the present invention for handling a minor amount of a biological material comprises a construct and a base, wherein: the base holds the construct in a releasable manner; the construct is held on a surface of the base in such a manner that a part of the construct is exposed externally on the outer face of the instrument; and at least a part of a surface of the constructs is exposed externally on the outer face of the instrument being fabricated so as to allow the adhesion of the minor amount of the biological material thereto.

    摘要翻译： 提供了一种处理少量生物材料的技术，其目的是通过控制每种不同细胞类型的细胞数量和二维或三维构型来选择性地观察单细胞之间的相互作用。 本发明提供一种用于处理少量生物材料的仪器和方法。 用于处理少量生物材料的本发明的仪器包括构建体和基底，其中：所述基座以可释放的方式保持构造; 将构造体保持在基部的表面上，使得构造体的一部分在仪器的外表面外部暴露; 并且构造物的表面的至少一部分在外部被暴露在被制造的器械的外表面上，以便允许少量的生物材料粘附到其上。