公开(公告)号：EP1966371A1

公开(公告)日：2008-09-10

申请号：EP06846013.8

申请日：2006-12-21

申请人： Zymogenetics, Inc.

发明人： MALLET, Robert, W. ,  STENLAND, Christopher, J. ,  BOONE, Jonathon, C. ,  FORSTROM, John, W. ,  DE JONGH, Karen, S.

IPC分类号： C12N9/00

CPC分类号： C12N9/6429 ,  C12Y304/21005

摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.

公开(公告)号：EP0804480A1

公开(公告)日：1997-11-05

申请号：EP95943915.0

申请日：1995-12-20

申请人： ZymoGenetics, Inc.

发明人： FORSTROM, John, W. ,  LOFTON-DAY, Catherine, E. ,  LOK, Si

IPC分类号： C12N15 ,  A61K38 ,  A61P7 ,  C07K1 ,  C07K7 ,  C07K14 ,  C12P21 ,  C12R1

CPC分类号： C07K14/715 ,  A61K38/00 ,  C07K14/52

摘要： Purified mammalian thrombopoietin proteins and methods of making them are disclosed. The proteins are characterized by a Mr=70,000 ± 10,000 daltons as determined by SDS-polyacrylamide gel electrophoresis under denaturing conditions and are at least 90 % pure with respect to contaminating proteins. The proteins can be prepared by a method in which thrombopoietin is adsorbed to and eluted from a polypeptide comprising a ligand-binding domain of an MPL receptor, then the eluted thrombopoieting is fractionated by anion exchange chromatography.

公开(公告)号：EP0839158B1

公开(公告)日：2005-12-28

申请号：EP96920393.4

申请日：1996-05-22

申请人： ZymoGenetics, Inc.

发明人： ALASKA, Andrew, A. ,  CHANG, Jin-Jyi ,  DOWNEY, William ,  FORSTROM, John, W. ,  PHAN, Linh

IPC分类号： C07K14/52

CPC分类号： C07K14/524

摘要： Methods of removing protein contaminants from a solution comprising thrombopoietin are disclosed. The solution is exposed to hydroxyapatite, whereby protein contaminants are bound to the hydroxyapatite and the thrombopoietin remains substantially unbound, and the unbound thrombopoietin is recovered. The use of hydroxyapatite is advantageously combined with other purification and concentration techniques, including ion-exchange chromatography, ligand affinity chromatography, hydrophobic interaction chromatography, ultrafiltration, and differential precipitation.

公开(公告)号：EP0506820A1

公开(公告)日：1992-10-07

申请号：EP91901983.0

申请日：1990-12-21

申请人： ZYMOGENETICS, INC. ,  THE BOARD OF REGENTS OF THE UNIVERSITY OF WASHINGTON

发明人： FORSTROM, John, W. ,  GOODMAN, Richard, B. ,  MARTIN, Thomas, R.

IPC分类号： A61K38 ,  A61P29 ,  C07K1 ,  C07K5 ,  C07K7 ,  C07K14 ,  C12N15 ,  C12P21 ,  C12R1

CPC分类号： C07K14/47 ,  A61K38/00 ,  C07K14/52

摘要： Protéines porcines pures présentant une activité chimioattractive vis-à-vis des neutrophiles, et procédés de préparation des protéines. Ces protéines sont utiles dans des compositions augmentant la chimiotaxis des neutrophiles, comme pour la stimulation de la formation de tissus de granulations. On peut également utiliser les protéines dans des procédés de développement d'agents inflammatoires et anti-inflammatoires, parmi lesquels des peptides inhibant la chimiotaxis des neutrophiles.

公开(公告)号：EP1966371B1

公开(公告)日：2011-08-24

申请号：EP06846013.8

申请日：2006-12-21

申请人： Zymogenetics, Inc.

发明人： MALLET, Robert, W. ,  STENLAND, Christopher, J. ,  BOONE, Jonathon, C. ,  FORSTROM, John, W. ,  DE JONGH, Karen, S.

IPC分类号： C12N9/00

CPC分类号： C12N9/6429 ,  C12Y304/21005

摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.

公开(公告)号：EP0804480B1

公开(公告)日：2006-03-08

申请号：EP95943915.9

申请日：1995-12-20

申请人： ZymoGenetics, Inc.

发明人： FORSTROM, John, W. ,  LOFTON-DAY, Catherine, E. ,  LOK, Si

IPC分类号： C07K14/52 ,  A61K38/19

CPC分类号： C07K14/715 ,  A61K38/00 ,  C07K14/52

摘要： Purified mammalian thrombopoietin proteins and methods of making them are disclosed. The proteins are characterized by a Mr=70,000 +/- 10,000 daltons as determined by SDS-polyacrylamide gel electrophoresis under denaturing conditions and are at least 90 % pure with respect to contaminating proteins. The proteins can be prepared by a method in which thrombopoietin is adsorbed to and eluted from a polypeptide comprising a ligand-binding domain of an MPL receptor, then the eluted thrombopoieting is fractionated by anion exchange chromatography.

摘要翻译： 公开了纯化的哺乳动物血小板生成蛋白及其制备方法。 蛋白质的特征在于Mr = 70,000 +/- 10,000道尔顿，在变性条件下通过SDS-聚丙烯酰胺凝胶电泳测定，并且相对于污染蛋白质至少为90％纯度。 蛋白质可以通过其中血小板生成素被吸附到包含MPL受体的配体结合结构域的多肽洗脱的方法来制备，然后通过阴离子交换色谱分离洗脱的血小板生成素。

公开(公告)号：EP0839158A1

公开(公告)日：1998-05-06

申请号：EP96920393.0

申请日：1996-05-22

申请人： ZymoGenetics, Inc.

发明人： ALASKA, Andrew, A. ,  CHANG, Jin-Jyi ,  DOWNEY, William ,  FORSTROM, John, W. ,  PHAN, Linh

IPC分类号： C07K1 ,  C07K14

CPC分类号： C07K14/524

摘要： Methods of removing protein contaminants from a solution comprising thrombopoietin are disclosed. The solution is exposed to hydroxyapatite, whereby protein contaminants are bound to the hydroxyapatite and the thrombopoietin remains substantially unbound, and the unbound thrombopoietin is recovered. The use of hydroxyapatite is advantageously combined with other purification and concentration techniques, including ion-exchange chromatography, ligand affinity chromatography, hydrophobic interaction chromatography, ultrafiltration, and differential precipitation.