公开(公告)号：EP1702206A1

公开(公告)日：2006-09-20

申请号：EP04802349.3

申请日：2004-12-22

申请人： Axela Biosensors Inc.

发明人： CRACAUER, Raymond, Francis ,  GANSKE, Rocky ,  LIEDERMAN, Adam, Brian

IPC分类号： G01N21/01 ,  G01N21/07 ,  G01N37/00 ,  B01L3/00 ,  B01L3/14

CPC分类号： G01N21/4788 ,  B01L3/502715 ,  B01L3/508 ,  B01L3/5082 ,  B01L3/5085 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0406 ,  G01N21/07 ,  G01N2021/0321

摘要： The present invention provides disposable, semi-reusable, or single use reaction vessels with integrated optical elements for use with diffraction based assay systems. The vessel for assaying liquids for analytes includes a housing having at least one chamber or well for receiving a liquid therein and an optical element integrally formed with the housing for directing an incident light beam towards the well or chamber and directing a light beam away from the chamber after the light beam has interacted with analytes present in the liquid. The vessel may be test tube such as a blood collection tube, with or without, an optical element but having a pattern of analyte-specific receptors located on an inner surface of the tube wall so that when a liquid is introduced into the interior of the test tube analytes present in the liquid can bind with the pattern of analyte-specific receptors.

公开(公告)号：EP1701849A1

公开(公告)日：2006-09-20

申请号：EP04802350.1

申请日：2004-12-22

申请人： Axela Biosensors Inc.

发明人： CRACAUER, Raymond, Francis ,  GANSKE, Rocky ,  GOH, M., Cynthia ,  GOH, Jane, B. ,  LIEDERMAN, Adam, Brian ,  LOO, Richard ,  TAM, Pui

IPC分类号： B41K3/00

CPC分类号： G03F7/0002 ,  B41F17/006 ,  B41K1/28 ,  B82Y10/00 ,  B82Y40/00

摘要： The present invention provides an apparatus for producing patterns on a surface of a substrate. The apparatus includes a rigid support member such as a rigid tubular structure having first and second opposed ends and a fluid flow passageway extending therethrough. A printing stamp is attached at one of the opposed ends of the rigid support member. The printing stamp has a flexible diaphragm portion which has an outer surface which is coated with one or more materials in a pre-selected pattern. A pneumatic pressurizing mechanism communicating with an inner surface of the flexible diaphragm portion through the fluid flow passageway is used to bias the flexible diaphragm portion outwardly into intimate and substantially uniform contact with the surface of the substrate for transferring the pre-selected pattern onto said surface. The rigid tubular supports are attached to a robotic positioning mechanism for providing control of positioning of the stamp relative to the substrate surface.

公开(公告)号：EP1789795A1

公开(公告)日：2007-05-30

申请号：EP05772167.2

申请日：2005-08-04

申请人： Axela Biosensors Inc.

发明人： GOH, M., Cynthia ,  GOH, Jane, B. ,  LOO, Richard

IPC分类号： G01N33/543 ,  G01N21/47 ,  C12Q1/68

CPC分类号： G01N33/54353 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00637 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  G01N21/05 ,  G01N21/4788 ,  G01N21/77 ,  G01N33/54373 ,  G01N2021/7709

摘要： Fabrication of surfaces patterned with chemical crosslinkers for solution-phase immobilization of probe molecules and their use in diffraction-based sensing. In one embodiment of the invention, a chemical crosslinker, X1 - R1 -Y1, is deposited on areas of the substrate surface that defines a pattern and allowed to react with the surface for a sufficient period of time to attain the desired density of covalently linked crosslinkers on the surface. The reaction between the crosslinker X1 - R1 - Y1 and the surface can be accelerated using known techniques such as heating, microwave irradiation, sonication, etc, to achieve the desired density in less time. In another embodiment of the invention, two or more other types of cross-linkers may also be laid down in patterns on the surface to detect for two or more other types of molecules in solution.

公开(公告)号：EP1434994A2

公开(公告)日：2004-07-07

申请号：EP02759993.5

申请日：2002-09-13

申请人： Axela Biosensors Inc.

发明人： GOH, M., Cynthia ,  LOO, Richard ,  GOH, Jane, B. ,  MCALONEY, Richard

IPC分类号： G01N33/543

CPC分类号： G01N33/54373 ,  Y10S435/808 ,  Y10S436/805

摘要： The present invention relates to a method and apparatus for detecting analytes in a medium, and more particularly the present invention relates to an assay based on light diffraction which appears or changes upon the binding of analytes to their specific receptors laid out in patterns on a substrate, which has high sensitivity due to the appropriate choice of such patterns. The present invention is based on the principle that the pattern of recognition elements, which gives rise to the diffraction of the incident light in a diffraction-based assay, can be chosen in such a way so as to facilitate detection, and to enhance the signal to be detected compared to known gratings such as parallel straight lines. In one aspect the substrate itself has a surface topography designed to enhance the diffraction pattern signals. In another aspect the substrate is a diffractive optic element having the analyte-specific receptors affixed to the optic element. In another aspect the diffractive optic element is used as a master stamp for producing patterns of analyte-specific receptors which give the signal enhancements.