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公开(公告)号：EP3323896A2
公开(公告)日：2018-05-23
申请号：EP17183834.5
申请日：2012-01-30
申请人： Olink Proteomics AB
IPC分类号： C12Q1/68 , G01N33/542 , G01N33/58
摘要： The present invention relates to a proximity probe based detection assay ("proximity assay") for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific "background" signals, wherein the improvement comprises the use in such assays of a component comprising 3' exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3' exonuclease activity; (d) extending the 3' end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the step may occur contemporaneously with or after step (c); and (e) amplifying and detecting the extension product.
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公开(公告)号：EP3323896B1
公开(公告)日：2020-05-27
申请号：EP17183834.5
申请日：2012-01-30
申请人： Olink Proteomics AB
IPC分类号： C12Q1/68 , G01N33/542 , G01N33/58
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公开(公告)号：EP3365460B1
公开(公告)日：2020-04-22
申请号：EP16784900.9
申请日：2016-10-21
申请人： Olink Proteomics AB
IPC分类号： C12Q1/6804 , C12Q1/6818 , G01N33/53
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公开(公告)号：EP3323896A3
公开(公告)日：2018-06-13
申请号：EP17183834.5
申请日：2012-01-30
申请人： Olink Proteomics AB
IPC分类号： C12Q1/68 , G01N33/542 , G01N33/58
摘要： The present invention relates to a proximity probe based detection assay ("proximity assay") for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific "background" signals, wherein the improvement comprises the use in such assays of a component comprising 3' exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3' exonuclease activity; (d) extending the 3' end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the step may occur contemporaneously with or after step (c); and (e) amplifying and detecting the extension product.
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公开(公告)号：EP2670860B1
公开(公告)日：2017-08-16
申请号：EP12702018.8
申请日：2012-01-30
申请人： Olink Proteomics AB
IPC分类号： C12Q1/68 , G01N33/542 , G01N33/58
CPC分类号： C12Q1/6848 , C12Q1/6804 , C12Q1/689 , C12Q2600/16 , G01N33/542 , G01N33/558 , C12Q2521/325 , C12Q2533/101
摘要： The present invention relates to a proximity probe based detection assay ("proximity assay") for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific "background" signals, wherein the improvement comprises the use in such assays of a component comprising 3' exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3' exonuclease activity; (d) extending the 3' end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the step may occur contemporaneously with or after step (c); and (e) amplifying and detecting the extension product.
摘要翻译： 本发明涉及用于样品中分析物特别是邻近探针延伸测定（PEA）的基于邻近探针的检测测定（“邻近测定”），尤其涉及用于减少非特异性“背景 “信号，其中所述改进包括在这种测定中使用包含3'外切核酸酶活性的组分，所述方法包括：（a）使所述样品与至少一组至少第一和第二邻近探针接触，所述探针各自包含 分析物结合结构域和核酸结构域，并且可以同时结合分析物; （b）使所述邻近探针与所述分析物结合时，使所述邻近探针的核酸结构域彼此相互作用，其中所述相互作用包括形成双链体; （c）使所述样品与包含3'外切核酸酶活性的组分接触; （d）延伸所述双链体的至少一个核酸域的3'末端以产生延伸产物，其中所述步骤可以与步骤（c）同时或之后发生; 和（e）放大并检测延伸产物。
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公开(公告)号：EP3365460A1
公开(公告)日：2018-08-29
申请号：EP16784900.9
申请日：2016-10-21
申请人： Olink Proteomics AB
摘要： The present invention provides a plurality of pairs of proximity probes, each pair being capable of binding to a different target analyte, wherein the first and second proximity probes of each pair of probes comprise universal oligonucleotides conjugated to their analyte binding moieties, and hybridised to the universal oligonucleotides are different tag oligonucleotides comprising universal complement domains common to all tag oligonucleotides and unique domains unique to each tag oligonucleotide, as well as methods for their production.
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