公开(公告)号：EP2519635B1

公开(公告)日：2018-11-21

申请号：EP10844276.5

申请日：2010-12-28

申请人： Sanofi Vaccine Technologies, S.A.S.

发明人： BAYNE, Anne-Cecile V. ,  LIPPMEIER, James Casey ,  APT, Kirk Emil ,  ZIRKLE, Ross Eric

IPC分类号： C12N15/44

CPC分类号： C12P21/02 ,  A61K39/145 ,  A61K2039/5258 ,  C07K14/005 ,  C12N1/12 ,  C12N7/00 ,  C12N9/2402 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16152 ,  C12Y302/01018

摘要： The present invention relates to recombinant microalgal cells and their use in heterologous protein production, methods of production of heterologous polypeptides in microalgal extracellular bodies, microalgal extracellular bodies comprising heterologous polypeptides, and compositions comprising the same.

公开(公告)号：EP3222730A4

公开(公告)日：2018-06-20

申请号：EP16723238

申请日：2016-01-06

申请人： UNIV SANTIAGO CHILE

发明人： BELTRÁN PAVEZ CAROLINA ,  CORTEZ SAN MARTÍN MARCELO ,  SPENCER OSSA EUGENIO ,  TAMBLEY ZAMORANO CAROLINA ,  TORO ASCUY DANIELA

IPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/01 ,  C12N7/02 ,  C12N15/85

CPC分类号： C12N7/00 ,  C12N15/85 ,  C12N15/86 ,  C12N2760/16021 ,  C12N2760/16022 ,  C12N2760/16034 ,  C12N2760/16043 ,  C12N2760/16051 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2800/107 ,  C12N2830/80

摘要： The present invention describes a viral RNA expression plasmid and a method for obtaining viral particles based on said plasmids comprising transfecting animal cells with an expression vector or a set of expression vectors capable of expressing a nucleoprotein and RNA-dependent polymerase RNA; and transfecting the animal cell with an expression vector or a set of expression vectors with nucleotide sequences encoding recombinant RNA molecules.

公开(公告)号：EP3222730A1

公开(公告)日：2017-09-27

申请号：EP16723238.8

申请日：2016-01-06

申请人： Universidad de Santiago de Chile

发明人： BELTRÁN PAVEZ, Carolina ,  CORTEZ SAN MARTÍN, Marcelo ,  SPENCER OSSA, Eugenio ,  TAMBLEY ZAMORANO, Carolina ,  TORO ASCUY, Daniela

IPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/01 ,  C12N7/02

CPC分类号： C12N7/00 ,  C12N15/85 ,  C12N15/86 ,  C12N2760/16021 ,  C12N2760/16022 ,  C12N2760/16034 ,  C12N2760/16043 ,  C12N2760/16051 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2800/107 ,  C12N2830/80

摘要： The present invention describes a viral RNA expression plasmid and a method for obtaining viral particles based on said plasmids comprising transfecting animal cells with an expression vector or a set of expression vectors capable of expressing a nucleoprotein and RNA-dependent polymerase RNA; and transfecting the animal cell with an expression vector or a set of expression vectors with nucleotide sequences encoding recombinant RNA molecules.

摘要翻译： 本发明描述了病毒RNA表达质粒和基于所述质粒获得病毒颗粒的方法，包括用表达载体或能够表达核蛋白和RNA依赖性聚合酶RNA的一组表达载体转染动物细胞; 以及用具有编码重组RNA分子的核苷酸序列的表达载体或一组表达载体转染动物细胞。

公开(公告)号：EP2687600A2

公开(公告)日：2014-01-22

申请号：EP12757721.1

申请日：2012-03-16

申请人： Korea Center for Disease Control and Prevention ,  Chungbuk National University

发明人： CHOI, Young Ki ,  KANG, Chun ,  HONG, Kee Jong ,  SONG, Min Suk ,  BAEK, Yun Hee

IPC分类号： C12N15/11 ,  C12N15/85 ,  C12N15/33 ,  C12N7/01

CPC分类号： C12N15/85 ,  C12N7/00 ,  C12N9/1252 ,  C12N2760/16051 ,  C12N2760/16052 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2830/85 ,  C12Y207/07007

摘要： The present invention relates to a pol I promoter derived from Vero cells and a recombinant vector containing the same. When the pol I promoter derived from Vero cells according to the present invention is utilized, viruses can be manufactured efficiently, and consequently, the manufacture of both seasonal influenza vaccine and pandemic vaccine can be prepared more quickly to usefully address either situation.

摘要翻译： 本发明涉及从Vero细胞衍生的pol I启动子和含有它的重组载体。 当利用衍生自根据本发明的Vero细胞的pol I启动子时，可以有效地制造病毒，因此可以更迅速地制备季节性流感疫苗和大流行性流感疫苗，以有效地解决任一情况。

公开(公告)号：EP2614140A2

公开(公告)日：2013-07-17

申请号：EP11823761.9

申请日：2011-09-06

申请人： SK Chemicals, Co., Ltd.

发明人： PARK, Yong Wook ,  LEE, Kun Se ,  LEE, Bong-yong ,  PARK, Mahnhoon ,  KIM, Hun ,  KIM, Yun-hee ,  LEE, Su-jeen

IPC分类号： C12N5/071 ,  C12N5/02 ,  A61K39/145

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/525 ,  A61K2039/5254 ,  C12N5/0686 ,  C12N2500/90 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2760/16171 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/16252 ,  C12N2760/16271 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/466

摘要： Disclosed is a Madin-Darby canine kidney (MDCK)-derived cell line. The MDCK-derived cell line is derived from MDCK cells deposited under accession number ATCC CCL-34. The MDCK-derived cell line can be prepared by serum-free culture and suspension culture. Preferably, the MDCK-derived cell line has low or no tumorigenicity. The MDCK-derived cell line is preferably selected from MDCK Sky1023, MDCK Sky10234 and MDCK Sky3851. Further disclosed are a culture method for growing the MDCK-derived cells and a method for producing a vaccine virus using the MDCK-derived cells.

公开(公告)号：EP2576773A2

公开(公告)日：2013-04-10

申请号：EP11727412.6

申请日：2011-06-06

申请人： Avir Green Hills Biotechnology Research Development Trade AG

发明人： MUSTER, Thomas ,  EGOROV, Andrej ,  WOLSCHEK, Markus

IPC分类号： C12N7/02 ,  C12N15/86 ,  C07K14/11 ,  C12N15/09

CPC分类号： C12N7/00 ,  C07K2319/50 ,  C12N7/02 ,  C12N15/86 ,  C12N2760/00011 ,  C12N2760/00052 ,  C12N2760/16111 ,  C12N2760/16134 ,  C12N2760/16152

摘要： The present invention provides a method for generating negative-stranded segmented RNA viruses using linear expression constructs in the presence of helper virus which comprises at least one amino acid modification within the N-terminal cytoplasmic region of the NA protein.

公开(公告)号：EP2272950A3

公开(公告)日：2011-07-20

申请号：EP10182312.8

申请日：2005-11-08

申请人： Abbott Biologicals B.V. ,  Erasmus University Medical Center Rotterdam

发明人： de Wit, Emmie ,  Spronken, Monique I.J. ,  Fouchier, Ron A.M. ,  Osterhaus, Albert D.M.E.

IPC分类号： C12N7/04 ,  C12N7/02 ,  C12N5/10 ,  C12N15/86 ,  A61K39/145 ,  A61K48/00

CPC分类号： C12N7/00 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/5258 ,  C07K14/005 ,  C12N15/86 ,  C12N2760/16122 ,  C12N2760/16143 ,  C12N2760/16152 ,  C12N2760/16162

摘要： The invention relates to the field of influenza virus and the vaccination against flu. The invention provides a conditionally defective influenza virus particle having seven different influenza nucleic acid segments. The invention also provides a conditionally defective influenza virus particle lacking an influenza nucleic acid segment selected from the group of segments essentially encoding acidic polymerase (PA), the basic polymerase 1 (PB1) and the basic polymerase 2 (PB2). In particular, the invention provides defective influenza virus particles having seven different influenza nucleic acid segments and lacking an influenza nucleic acid segment essentially encoding acidic polymerase. Furthermore, the invention provides use of a composition comprising a defective influenza virus particle according to the invention for the production of a pharmaceutical composition directed at generating immunological protection against infection of a subject with an influenza virus, and provides a method for generating immunological protection against infection of a subject with an influenza virus comprising providing a subject in need thereof with a composition comprising such defective influenza virus particle.

公开(公告)号：EP2069480A4

公开(公告)日：2011-03-30

申请号：EP07873789

申请日：2007-09-14

申请人： MEDIMMUNE LLC

发明人： LIU JONATHAN ,  SCHWARTZ RICHARD ,  THOMPSON MARK ,  MARANGA LUIS ,  HSU SIMON ,  GHOSH MRIDUL ,  SUBRAMANIAN AJIT

IPC分类号： C12N5/10

CPC分类号： C12N7/00 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2760/16251 ,  C12N2760/16252

公开(公告)号：EP3341018A1

公开(公告)日：2018-07-04

申请号：EP16778485.9

申请日：2016-08-25

申请人： Wisconsin Alumni Research Foundation

发明人： KAWAOKA, Yoshihiro ,  NEUMANN, Gabriele

IPC分类号： A61K39/12 ,  C12N7/00

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5254 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2760/16223 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/16252

摘要： Compositions and methods to prepare influenza virus-like particles (VLPs) are provided.

公开(公告)号：EP2571520B1

公开(公告)日：2018-04-04

申请号：EP11725975.4

申请日：2011-05-20

申请人： Seqirus UK Limited

发明人： TRUSHEIM, Heidi ,  MASON, Peter ,  FRANTI, Michael ,  KEINER, Bjoern ,  SACKAL, Melissa ,  HUNZIKER, Juerg ,  MORRISSEY, David ,  NATT, François Jean-Charles

IPC分类号： A61K39/145 ,  C12N15/113 ,  C12N7/02

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/525 ,  C12N15/1131 ,  C12N2310/14 ,  C12N2320/00 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16152

摘要： Methods for producing reassortant viruses are provided wherein the transcription and/or translation of the hemagglutinin and/or neuraminidase genes are suppressed.