公开(公告)号：EP2663328A4

公开(公告)日：2014-09-10

申请号：EP12734409

申请日：2012-01-10

申请人： US GOVERNMENT ,  ROYAL CORNWALL HOSPITAL TRUST

发明人： EMERSON SUZANNE U ,  SHUKLA PRIYANKA ,  NGUYEN HANH T ,  PURCELL ROBERT H ,  DALTON HARRY R ,  BENDALL RICHARD P

IPC分类号： A61K39/29

CPC分类号： C12N7/00 ,  A61K39/00 ,  C07K14/005 ,  C07K14/47 ,  C12N15/86 ,  C12N2770/28121 ,  C12N2770/28122 ,  C12N2770/28134 ,  C12N2770/28143 ,  C12N2770/28151 ,  C12N2770/28152 ,  C12Q1/707

摘要： The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus. The invention relates, in part, on the identification of a new strain of HEV genotype 3 virus. Strain Kernow-C1 (genotype 3) of HEV, which was isolated from a chronically infected patient, was used to identify human, pig and deer cell lines permissive for infection. Adaptation of the Kernow-C 1 strain to growth in human hepatoma cells selected for a rare virus recombinant that contained an insertion of 174 ribonucleotides (58 amino acids) of a human ribosomal protein gene and additional mutations.

公开(公告)号：EP2663328B1

公开(公告)日：2018-09-19

申请号：EP12734409.1

申请日：2012-01-10

申请人： The Government of The United States of America, as represented by The Secretary, Department of Health and Human Services ,  Royal Cornwall Hospital Trust

发明人： EMERSON, Suzanne U. ,  SHUKLA, Priyanka ,  NGUYEN, Hanh T. ,  PURCELL, Robert H. ,  DALTON, Harry R. ,  BENDALL, Richard P.

IPC分类号： C12N7/00 ,  A61K39/29 ,  C07K14/08 ,  C12Q1/70

CPC分类号： C12N7/00 ,  A61K39/00 ,  C07K14/005 ,  C07K14/47 ,  C12N15/86 ,  C12N2770/28121 ,  C12N2770/28122 ,  C12N2770/28134 ,  C12N2770/28143 ,  C12N2770/28151 ,  C12N2770/28152 ,  C12Q1/707

摘要： The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus. The invention relates, in part, on the identification of a new strain of HEV genotype 3 virus. Strain Kernow-C1 (genotype 3) of HEV, which was isolated from a chronically infected patient, was used to identify human, pig and deer cell lines permissive for infection. Adaptation of the Kernow-C 1 strain to growth in human hepatoma cells selected for a rare virus recombinant that contained an insertion of 174 ribonucleotides (58 amino acids) of a human ribosomal protein gene and additional mutations.

公开(公告)号：EP2663328A1

公开(公告)日：2013-11-20

申请号：EP12734409.1

申请日：2012-01-10

申请人： The Government of The United States of America, as represented by The Secretary, Department of Health and Human Services ,  Royal Cornwall Hospital Trust

发明人： EMERSON, Suzanne U. ,  SHUKLA, Priyanka ,  NGUYEN, Hanh T. ,  PURCELL, Robert H. ,  DALTON, Harry R. ,  BENDALL, Richard P.

IPC分类号： A61K39/29

CPC分类号： C12N7/00 ,  A61K39/00 ,  C07K14/005 ,  C07K14/47 ,  C12N15/86 ,  C12N2770/28121 ,  C12N2770/28122 ,  C12N2770/28134 ,  C12N2770/28143 ,  C12N2770/28151 ,  C12N2770/28152 ,  C12Q1/707

摘要： The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus. The invention relates, in part, on the identification of a new strain of HEV genotype 3 virus. Strain Kernow-C1 (genotype 3) of HEV, which was isolated from a chronically infected patient, was used to identify human, pig and deer cell lines permissive for infection. Adaptation of the Kernow-C 1 strain to growth in human hepatoma cells selected for a rare virus recombinant that contained an insertion of 174 ribonucleotides (58 amino acids) of a human ribosomal protein gene and additional mutations.

公开(公告)号：EP1169341A1

公开(公告)日：2002-01-09

申请号：EP01917440.8

申请日：2001-02-05

申请人： Department of Science and Technology ,  All India Institute of Medical Sciences

发明人： PANDA, Subrat Kumar ,  ANSARI, Israrul Haque,Dept. of Pathology ,  AGRAWAL, Shripa ,  DURGAPAL, Hemlata

IPC分类号： C07K14/08 ,  C12N15/51 ,  C12N7/04 ,  G01N33/576 ,  A61K39/29

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K2039/525 ,  C07K14/005 ,  C12N2770/28121 ,  C12N2770/28162

摘要： The present invention relates to a unique genetically engineered nucleic acid clone of hepatitis E virus (HEV) which comprises the complete structural (ORF2 and ORF3) and non-stuctural (ORF1) genes, viz. the complete coding sequence, of HEV, flanked by 5` and 3`non-coding regions along with a 3`polyadenylated tail to the 3` non-coding region, said clone being capable of infecting cultured liver cells and replicating, transcribing and assembling HEV therein. The invention also incorporates within its scope a process for synthesising such clone and the expression therefrom of viral proteins adapted for use in the detection of the presence of antibodies to HEV in biological samples. Other uses to which the clone and the expressed viral proteins can be put include a diagnostic test kit for the presence of HEV infection comprising said viral proteins, and HEV vaccine comprising non-infectious HEV particles produced from a mutation of said nucleic acid clone of hepatitis E virus and an assay system for rapid anti-HEV drug testing.