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    Endothelin,converting enzymes
    2.
    发明公开
    Endothelin,converting enzymes 失效
    内皮素，转化酶

    公开(公告)号：EP0545344A3

    公开(公告)日：1994-02-16

    申请号：EP92120430.1

    申请日：1992-11-30

    申请人： NISSHIN FLOUR MILLING CO., LTD.

    发明人： Ohwaki, Tatsuya, Kohpo-Hanafusa 203 ,  Sakai, Hiroshi

    IPC分类号： C12N9/64

    CPC分类号： C12N9/6497 ,  C12Y304/24071

    摘要： Endothelin converting enzymes of mammalian cell origin or mammalian blood origin are disclosed, which have the activity of converting big endothelin to endothelin and a hydrated density of up to about 0.94 g/ml, about 0.94 g/ml to about 1.006 g/ml, about 1.006 g/ml to about 1.063 g/ml and about 1.063 g/ml to about 1.210 g/ml. A process for preparing the enzymes from mammalian cells and mammalian blood by centrifugation or gel filtration process is also disclosed.

    摘要翻译： 公开了哺乳动物细胞起源或哺乳动物血源的内皮素转化酶，其具有将大内皮素转化为内皮素的活性和约0.94g / ml，约0.94g / ml至约1.006g / ml，约 1.006克/毫升至约1.063克/毫升和约1.063克/毫升至约1.210克/毫升。 还公开了通过离心或凝胶过滤法从哺乳动物细胞和哺乳动物血液制备酶的方法。

    Endothelin,converting enzymes
    3.
    发明公开
    Endothelin,converting enzymes 失效
    内皮素-konvertierende酶。

    公开(公告)号：EP0545344A2

    公开(公告)日：1993-06-09

    申请号：EP92120430.1

    申请日：1992-11-30

    申请人： NISSHIN FLOUR MILLING CO., LTD.

    发明人： Ohwaki, Tatsuya, Kohpo-Hanafusa 203 ,  Sakai, Hiroshi

    IPC分类号： C12N9/64

    CPC分类号： C12N9/6497 ,  C12Y304/24071

    摘要： Endothelin converting enzymes of mammalian cell origin or mammalian blood origin are disclosed, which have the activity of converting big endothelin to endothelin and a hydrated density of up to about 0.94 g/ml, about 0.94 g/ml to about 1.006 g/ml, about 1.006 g/ml to about 1.063 g/ml and about 1.063 g/ml to about 1.210 g/ml. A process for preparing the enzymes from mammalian cells and mammalian blood by centrifugation or gel filtration process is also disclosed.

    摘要翻译： 公开了内皮素转化哺乳动物细胞来源或哺乳动物血源的酶，其具有将大内皮素转化为内皮素的活性，水合密度高达约0.94g / ml，约0.94g / ml至约1.006g / ml，约为 1.006g / ml至约1.063g / ml和约1.063g / ml至约1.210μg/ ml。 还公开了通过离心或凝胶过滤方法从哺乳动物细胞和哺乳动物血液制备酶的方法。

    Endothelin converting enzymes and processes for preparing them
    7.
    发明公开
    Endothelin converting enzymes and processes for preparing them 失效
    内皮素umwandelndes Enzym und Verfahren zu seiner Herstellung。

    公开(公告)号：EP0638643A1

    公开(公告)日：1995-02-15

    申请号：EP94305768.7

    申请日：1994-08-03

    申请人： NISSHIN FLOUR MILLING CO., LTD.

    发明人： Ohwaki, Tatsuya, c/o Nisshin Flour M. Co. Ltd. ,  Sakai, Hiroshi, c/o Nisshin Flour M. Co. Ltd.

    IPC分类号： C12N9/64

    CPC分类号： C12N9/6497 ,  C12Y304/24071

    摘要： Endothelin converting enzymes of human blood origin or human cells origin are disclosed, which have the following properties:

    (1) Action: being capable of converting big endothelin to endothelin;
    (2) Substrate specificity: acting on big endothelin-1, big endothelin-2, or big endothelin-3;
    (3) Optimum pH: pH 6.5-7.5;
    (4) Molecular weight: about 5.4 x 10⁵ as measured on gel-filtration using a column of crosslinked agarose gel bead (exclusion limit 5 x 10⁶ Daltons);
    (5) Inhibitors: inhibited by ethylenediaminetetraacetate (EDTA), phosphoramidon, thiorphan, phenylmethanesulfonyl fluoride (PMSF), and chymostatin;
    (6) Hydrated density: not greater than about 1.210 g/ml;
    (7) Formation of precipitate: precipitation by adding 0.65% dextran sulfate (M.W. 500,000) and 0.2 M manganese chloride;
    (8) Influence of oxidation: inactivated by oxidation by copper ion.

    摘要翻译： 公开了人血源或人细胞来源的内皮素转化酶，其具有以下性质：（1）作用：能够将大内皮素转化为内皮素; （2）底物特异性：作用于大内皮素-1，大内皮素-2或大内皮素-3; （3）最适pH：pH 6.5-7.5; （4）使用交联的琼脂糖凝胶珠柱（排除极限5×10 6道尔顿）在凝胶过滤上测量的分子量：约5.4×10 5; （5）抑制剂：乙二胺四乙酸（EDTA），磷酰胺，噻吩，苯基甲磺酰氟（PMSF）和菊苣素抑制; （6）水合密度：不大于约1.210g / ml; （7）沉淀物的形成：通过加入0.65％硫酸葡聚糖（M.W.500,000）和0.2M氯化锰沉淀： （8）氧化的影响：被铜离子氧化灭活。