利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

23 条记录 (耗时 0.011 秒)

    1-Naphtholphthaleinmonophosphate, Verfahren zu deren Herstellung sowie deren Verwendung zur Bestimmung der alkalischen Phosphatase
    4.
    发明公开
    1-Naphtholphthaleinmonophosphate, Verfahren zu deren Herstellung sowie deren Verwendung zur Bestimmung der alkalischen Phosphatase 失效
    1-萘酚酞单磷酸酯，其制备方法以及它们用于碱性磷酸酶的确定的用途。

    公开(公告)号：EP0316791A2

    公开(公告)日：1989-05-24

    申请号：EP88118800.7

    申请日：1988-11-11

    申请人： BOEHRINGER MANNHEIM GMBH

    发明人： Güthlein, Werner, Dr. rer. nat. ,  Merdes, Hartmut, Dr. rer. nat.

    IPC分类号： C07F9/12 ,  C12Q1/42

    CPC分类号： C12Q1/42 ,  C07F9/65517 ,  C12Q2334/40

    摘要： Beansprucht werden neue 1-Naphtholphthaleinmonophosphate sowie ein Verfahren zu deren Herstellung. Hierzu werden Naphtholphthaleine mit Phosphorverbindungen in Gegenwart einer organischen Base umgesetzt und gegebenenfalls bromiert.
    Die neuen Verbindungen werden von alkalischen Phosphatasen gespalten und eignen sich demnach als Substrate für die Bestimmung der Aktivität von alkalischen Phosphatasen. Die als Spaltprodukte entstehenden 1-Naphtholphthalein-Derivate können photometrisch bestimmt werden.

    摘要翻译： 1-萘酚酞单磷酸酯是通过使用在有机碱的存在下的磷化合物naphtholphthaleins制备，并且如果需要清除，它们的溴化。 ... 通过碱性磷酸酶裂解的化合物，并因此适合于作为底物碱性磷酸酶活性的测定。 形成为裂解产物的1-萘酚酞衍生物可通过光度测定来确定的开采。

    Method for stabilizing solutions of gamma-glutamyl-p-nitroanilide
    8.
    发明公开
    Method for stabilizing solutions of gamma-glutamyl-p-nitroanilide 失效
    Verfahren zur Stabilisierung vonLösungenvon Gamma-Glutamyl-p-nitroanilid。

    公开(公告)号：EP0006582A2

    公开(公告)日：1980-01-09

    申请号：EP79102058.9

    申请日：1979-06-21

    申请人： Modrovich, Ivan Endre

    发明人： Modrovich, Ivan Endre

    IPC分类号： G01N33/50 ,  G01N33/96 ,  C07C103/50

    CPC分类号： G01N33/96 ,  C12Q2334/40 ,  C12Q2337/12 ,  G01N33/50 ,  G01N33/72 ,  G01N33/82 ,  G01N33/84 ,  G01N33/92 ,  G01N2333/904 ,  G01N2333/9108 ,  G01N2333/9121

    摘要： A labile, organic reagent which is unstable in aqueous media and stable in a non-aqueous media is stabilized by dissolving the organic reagent in a water-miscible, organic solvent which is liquid at room temperature and which is nondegradatively reactive with the organic reagent to form a solution of the organic reagent in the organic solvent. At least 1 percent of an inert, high surface area particulate dessicant is added to the solution for entrapping water with the dessicant so that the residual water content of the solution is below about 0.5 percent. The dessieant can be removed from the solution before seating it. More than one organic reagent can be added to the solvent, and a solubilizing agent for the organic reagent can be used.

    摘要翻译： 在水性介质中不稳定并且在非水介质中稳定的不稳定的有机试剂通过将有机试剂溶解在与水相混溶的有机溶剂中而得到稳定，该有机溶剂在室温下为液体，并且与有机试剂非降解反应， 形成有机试剂在有机溶剂中的溶液。 向溶液中加入至少1％的惰性，高表面积的颗粒状干燥剂，以便用干燥剂捕获水，使得溶液的残留水含量低于约0.5％。 在密封之前，可以将干燥剂从溶液中取出。 可以向溶剂中加入多于一种的有机试剂，可以使用有机试剂的增溶剂。

    USE OF HALOALKYL DERIVATIVES OF REPORTER MOLECULES TO ANALYZE METABOLIC ACTIVITY IN CELLS.
    9.
    发明公开
    USE OF HALOALKYL DERIVATIVES OF REPORTER MOLECULES TO ANALYZE METABOLIC ACTIVITY IN CELLS. 失效
    使用报告分子的HALOALKYL衍生物来分析细胞中的代谢活性。

    公开(公告)号：EP0603266A4

    公开(公告)日：1997-01-15

    申请号：EP92919254

    申请日：1992-08-21

    申请人： MOLECULAR PROBES INC

    发明人： HAUGLAND RICHARD P

    IPC分类号： C12Q1/04 ,  C12Q1/34 ,  G01N33/535 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/37 ,  C12Q1/40 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q1/46 ,  C12Q1/54

    CPC分类号： C12Q1/04 ,  C12Q1/34 ,  C12Q2334/00 ,  C12Q2334/20 ,  C12Q2334/40 ,  C12Q2337/20 ,  G01N33/535 ,  G01N2333/924 ,  Y10S530/802

    摘要： The subject invention provides a method for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is present. In particular, improved retention results from a two part process involving conjugation of haloalkyl-substituted derivatives of a reporter molecule with intracellular cysteine-containing peptides while unblocking the reporter molecule. The method for analyzing metabolic activity of cells involves the use of a substrate having the form XR-REPORTER-BLOCK wherein -BLOCK is a group selected to be removable by action of a specific analyte, to give REPORTER spectral properties different from those of the substrate, -REPORTER- is a molecule that, when no longer bound to BLOCK by a BLOCK-REPORTER bond, has spectral properties different from those of the substrate, and XR- is a haloalkyl moiety that can covalently react with an intracellular thiol (Z-S-H) to form a thioether conjugate (Z-S-R-). After the substrate enters the cells, the analyte removes BLOCK to make REPORTER detectable by the change in spectral properties, and the haloalkyl XR reacts with the intracellular thiol to form the thioether conjugate inside the cells, which is well-retained in the cells.