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公开(公告)号：EP0585868A3
公开(公告)日：1994-11-23
申请号：EP93113891.1
申请日：1993-08-31
发明人： Sasaki, Motohiro , Matsukawa, Masahiko, Nippon Paint Co., Ltd. , Yoshioka, Katsuaki , Anan, Makoto , Isomura, Mitsuo, c/o FUJIREBIO INC. , Ashihara, Yoshihiro, c/o FUJIREBIO INC. , Okada, Masahisa, c/o FUJIREBIO INC.
IPC分类号： G01N33/546
CPC分类号： B03C1/01 , G01N33/5434 , G01N2446/10 , G01N2446/86 , G01N2446/90 , H01F1/36
摘要： There are disclosed a magnetic particle for an immunoassay method, which comprises a core and a coating layer formed on the surface of the core wherein said core comprises an organic polymer matrix and said coating layer comprises a mixed crystal ferrite represented by the formula:
M x Fe (3-x) O₄
wherein M represents at least one metal selected from the group consisting of Mn, Ni, Zn, Co, Cu, Mg, Sn, Ca and Cd, and x is a number satisfying the relation: 0
and an antigen or an antibody is bound onto the surface of the coating layer and wherein said particle has a particle size of 0.03 to 10 µm, and an immunoassay method using the same.-
2.发明公开
公开(公告)号：EP0344270A4
公开(公告)日：1992-12-09
申请号：EP89900419
申请日：1988-10-18
IPC分类号： G01N33/545 , A61M1/36 , B01J20/28 , C08F2/44 , C08F12/08 , C08F20/14 , C08F257/02 , C08F285/00 , C08L25/00 , C08L25/08 , C12N11/14 , C12Q1/34 , C12Q1/68 , G01N33/543 , G01N33/553 , G01N33/58 , C02F1/48
CPC分类号： B01J20/28016 , A61M1/3618 , B01J20/28004 , B01J20/28009 , C08F257/02 , C08F285/00 , C12Q1/6834 , G01N33/54326 , G01N33/5434 , G01N33/582 , G01N33/585 , G01N2446/10 , G01N2446/40 , G01N2446/84 , G01N2446/86 , C12Q2563/143 , C12Q2563/107
摘要： This invention provides a novel process of producing magnetically responsive polymer particles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used as core particles and transformed into magnetically responsive polymer particles. The surface of these magnetically responsive polymer particles can be coated further with another layer of functionalized polymer. These magnetically responsive polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of immunoassays, DNA/RNA hybridization probes assays, affinity purification, cell separation and other medical, diagnostic, and industrial applications.
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3.发明公开
公开(公告)号：EP0344270A1
公开(公告)日：1989-12-06
申请号：EP89900419.0
申请日：1988-10-18
IPC分类号： G01N33 , B01J20 , C08F2 , C08F12 , C08F20 , C08F257 , C08F285 , C08L25 , C12N11 , C12Q1 , A61M1
CPC分类号： B01J20/28016 , A61M1/3618 , B01J20/28004 , B01J20/28009 , C08F257/02 , C08F285/00 , C12Q1/6834 , G01N33/54326 , G01N33/5434 , G01N33/582 , G01N33/585 , G01N2446/10 , G01N2446/40 , G01N2446/84 , G01N2446/86 , C12Q2563/143 , C12Q2563/107
摘要： This invention provides a novel process of producing magnetically responsive polymer particles comprising polym eric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used as core particles and transformed into magneti cally responsive polymer particles. The surface of these magnetically responsive polymer particles can be coated further with another layer of functionalized polymer. These magnetically responsive polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of immunoassays, DNA/RNA hybridization probes assays, affinity purification, cell separa tion and other medical, diagnostic, and industrial applications.
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公开(公告)号：EP0007932A1
公开(公告)日：1980-02-20
申请号：EP78900004
申请日：1978-12-08
申请人： MOSBACH KLAUS H
发明人： MOSBACH KLAUS H
CPC分类号： A61K9/5094 , G01N2446/10
摘要： On produit des particules magnetiques de polymere a titre de support d'une substance presentant une activite biologique, et de preference des proprietes pharmaceutiques. Une methode de production de telles particules consiste a traiter un polymere de preference poreux et/ou un monomere polymerisable a l'aide d'une solution colloidale d'une substance magnetique, ladite substance etant deposee dans les pores ou dans le reseau du polymere. La substance active est associee au polymere avant ou apres magnetisation. La substance peut egalement etre ajoutee a un melange de monomere et de substance magnetique pour se deposer dans les pores ou dans le reseau du polymere en meme temps que la substance magnetique lors de la polymerisation. Les particules ainsi produites peuvent etre utilisees pour concentrer localement un medicament dans un organisme ou pour produire un faible debit de la substance a partir d'une reserve, les particules etant retenues par un champ magnetique dans une zone delimitee et, par exemple dans le cadre de la chromatographie par affinite et d'essais immunologiques tels que RIA, ELISA.
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5.发明授权
公开(公告)号：EP0709680B1
公开(公告)日：2001-09-26
申请号：EP94919875.8
申请日：1994-07-08
申请人： FUJIREBIO Inc.
IPC分类号： G01N33/553 , G01N33/548
CPC分类号： G01N33/5434 , G01N2446/10 , G01N2446/84 , G01N2446/90
摘要： This invention intends to provide an immunoassay for obtaining stable measuring results which provide low noises and high signals, characterized by use of gelatin magnetic particles whose particle diameters range from 1.0 to 10 mu m and each having a nucleus of polymer compound, a layer formed from a magnetic material so as to cover the exterior of the nucleus and gelatin covering the surface of the layer formed from the magnetic material.
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公开(公告)号：EP0879063A1
公开(公告)日：1998-11-25
申请号：EP95943894.0
申请日：1995-12-15
申请人： IGEN, INC.
发明人： MOSBACH, Klaus , KRIZ, Dario , ANSELL, Richard, J.
CPC分类号： B03C1/01 , A61K9/5094 , G01N33/5434 , G01N2446/10 , G01N2446/20 , G01N2446/40 , G01N2600/00 , H01F1/111
摘要： The present invention is broadly directed to magnetically susceptible polymer particles having specifically-tailored adsorptivities and to related processes involving the particles (e.g., making the particles; separating target compounds from other compounds using the particles; and delivering selected compounds to targeted areas of concentration using the particles). The designed adsorptivities of the particles arise from either (i) selected adsorbents in the particles; or (ii) recognition sites in the particles formed by the molecular imprinting polymerization reactions used to make the particles. Of particular interest are particles and processes that involve biologically active substances, e.g., pharmaceuticals.
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公开(公告)号：EP0840648A1
公开(公告)日：1998-05-13
申请号：EP97925131.0
申请日：1997-05-23
申请人： BIO MERIEUX
CPC分类号： H01F1/37 , B03C1/01 , G01N33/5375 , G01N33/5434 , G01N2446/10 , G01N2446/40 , G01N2446/84 , G01N2446/86 , H01F1/44 , Y10S436/806 , Y10S436/824 , Y10T428/2982 , Y10T428/2989 , Y10T428/2991 , Y10T428/2998 , Y10T436/24
摘要： The invention discloses superparamagnetic monodispersed particles, their method of preparation and their uses. These particles comprise: a core of a first polymer, an internal layer of a second polymer coating the core, and in which is distributed a magnetic material, and an external layer of a third polymer coating the magnetic layer, and capable of interacting with at least one biological molecule, at least the second polymer being heat sensitive and having a predetermined lower critical solubility temperature (LCST) of 15 to 65 °C.
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8.发明公开
公开(公告)号：EP0543988A4
公开(公告)日：1993-11-18
申请号：EP92914163
申请日：1992-06-15
IPC分类号： A01N1/02 , A61K47/48 , B03C1/01 , B03C1/30 , C02F1/58 , C07K17/00 , C08F2/44 , C08F291/00 , C12N1/00 , C12N5/00 , C12N5/06 , C12N11/08 , C12N11/14 , C12Q1/68 , G01N33/543 , G01N33/553 , H01F1/36 , H01F1/37
CPC分类号： H01F1/37 , A61K47/6923 , A61K47/6927 , A61K47/6933 , B03C1/01 , C07K17/00 , C12N11/08 , C12Q1/6834 , G01N33/5434 , G01N2446/10 , G01N2446/40 , H01F1/36
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公开(公告)号：EP0420186A3
公开(公告)日：1991-07-31
申请号：EP90118468.9
申请日：1990-09-26
发明人： Okada, Masahisa , Ashihara, Yoshihiro , Yano, Akira , Oishi, Masao , Yoshioka, Katsuaki , Nakamura, Toshiomi
IPC分类号： G01N33/553 , G01N33/546 , G01N33/545
CPC分类号： B03C1/01 , G01N33/5434 , G01N2446/10 , G01N2446/86 , G01N2446/90 , Y10S435/968 , Y10S436/806
摘要： There is disclosed an immunoassay method using magnetic particles comprising a core and a coating layer on the surface thereof, the improvement wherein the core comprises an organic polymer and the coating layer comprises an iron oxide type ferrite coating layer, an antigen or an antibody is bound onto the surface of the coating layer and the particle has a particle size of 0.2 to 3 µm.
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公开(公告)号：EP0007932B1
公开(公告)日：1983-08-24
申请号：EP78900004.9
申请日：1978-06-01
申请人： MOSBACH, Klaus H.
发明人： MOSBACH, Klaus H.
CPC分类号： A61K9/5094 , G01N2446/10
摘要： Magnetic polymer particles as carriers of biologically, preferably pharmaceutically active substance are produced. One process of producing the magnetic polymer particles is by treatment of a preferably porous polymer and/or polymerizable monomer with a colloidal solution of magnetic material, the magnetic material being deposited in the pores or lattice of the polymer. The active substance is associated with the polymer either before or after the magnetization. The substance can also be added to a mixture of monomer and magnetic material for deposition in the pores or lattice of the polymer together with the magnetic material at the polymerization. The produced particles can be used for local concentration of a medicine in an organism or for slow release from a deposit, the particles being retained by a magnetic field within a restricted area, and for example, in so-called affinity chromatography and immuno assays, such as RIA, ELISA.
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