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26 条记录 (耗时 0.008 秒)

    Method and compositions for protecting anerobic microorganisms
    1.
    发明公开
    Method and compositions for protecting anerobic microorganisms 失效
    用于保护无机微生物的方法和组合物

    公开(公告)号：EP0246912A3

    公开(公告)日：1988-11-02

    申请号：EP87304571

    申请日：1987-05-22

    申请人： SYNTEX (U.S.A.) INC.

    发明人： Armenta, Richard D. ,  Ullman, Edwin F. ,  Gibbons, Ian

    IPC分类号： C12M01/04 ,  C12N01/00 ,  C12N01/04 ,  C12N09/04

    CPC分类号： C12N1/04 ,  C12M23/08 ,  C12M23/32 ,  C12M23/34 ,  C12M23/38 ,  C12M41/34 ,  Y10S435/801 ,  Y10S435/818

    摘要： A method is disclosed for reducing the oxygen content of a medium in which are present cells, usually anaerobic microorganisms, to extend the time during which the cells remain viable. The method comprises having in fluid contact with the aqueous medium an effective amount of an oxidase and substrate for the oxidase. The oxidase and substrate for the oxidase in an aqueous medium can be in fluid, e.g., air, contact with a separately contained aqueous medium in which the cells are present. Alternatively, the cells can be present in the same aqueous medium as the oxidase and substrate for the oxidase. The aqueous medium containing the oxidase and the substrate for the oxidase can further contain a hydrogen peroxide scavenger in an effective amount. A composition in accordance with the invention comprises in a liquid medium viable cells, such as anaerobic microorganisms, an oxidase and its substrate in an amount sufficient to retain the viability of the cells, and a hydrogen peroxide scavenger in an amount sufficient to reduce the level of hydrogen peroxide generated by the oxidase. The method of the invention has particular application in transport media used for transporting microbiological specimens.

    Primary and secondary alcohol dehydrogenase enzymes and the use thereof
    2.
    发明公开
    Primary and secondary alcohol dehydrogenase enzymes and the use thereof 失效
    主要和次要酒精脱氢酶及其用途

    公开(公告)号：EP0098136A3

    公开(公告)日：1986-03-19

    申请号：EP83303694

    申请日：1983-06-27

    申请人： EXXON RESEARCH AND ENGINEERING COMPANY

    发明人： Hou, Ching-Tsang ,  Laskin, Allen I. ,  Patel, Ramesh Narsibhal

    IPC分类号： C12N09/04 ,  C12P07/24 ,  C12P07/26 ,  C12P07/40

    CPC分类号： C12N9/0006 ,  C12P7/24 ,  C12P7/26

    摘要： A newly discovered and isolated heat-stable NAD(P) + . linked secondary alcohol-specific dehydrogenase enzyme is derived from microorganisms grown under aerobic conditions in a culture medium containing a carbon-containing compound having at least two carbon atoms, preferably a C 2 -C 10 alkane or a C 2 -C 10 alkyl radical donating compound, as the major carbon and energy source. The purified form of this enzyme, in the presence of NAD(P) + , is capable of converting primary and secondary alcohols, diols and aldehydes to their corresponding oxidation products and acetone to propanol. In addition, a newly discovered NAD(P) + -linked primary alcohol dehydrogenase enzyme can be isolated from the same microorganism source.

    摘要翻译： 新发现和分离的热稳定的NAD（P）+连接的二醇特异性脱氢酶源自在需氧条件下生长的微生物，所述微生物在含有至少两个碳原子的含碳化合物的培养基中，优选 C2-C10烷烃或C2-C10烷基供体化合物，作为主要碳源和能源。 在NAD（P）+存在下，该酶的纯化形式能够将伯醇和仲醇，二醇和醛转化成其相应的氧化产物和丙酮转化为丙醇。 此外，新发现的NAD（P）+连接的伯醇脱氢酶可以从相同的微生物来源分离。

    Stabilized enzymes, their production and use
    5.
    发明公开
    Stabilized enzymes, their production and use 失效
    稳定的酶，其生产和使用

    公开(公告)号：EP0175582A3

    公开(公告)日：1988-09-07

    申请号：EP85306690

    申请日：1985-09-19

    申请人： CETUS CORPORATION

    发明人： Shaked, Ze'ev ,  Wolfe, Sidney Norman

    IPC分类号： C12N09/96 ,  C12N11/00 ,  C12N11/18 ,  C12P19/02 ,  C12N09/04 ,  C12N09/08

    CPC分类号： C12P19/02 ,  C12N9/96 ,  C12N11/02 ,  C12N11/18

    摘要： Catalase and pyranose-2-oxidase may be stabilized according to the invention by means of chemical treatment which render the enzymes resistant to thermal inactivation or inactivation of glucosone or both. Stabilized catalase crosslinked with diimido esters, such as dimethyl suberimidate and dimethyl adipimidate, is described. Stabilized pyranose-2-oxidase amidinated with an amidinating agent, such as ethyl acetimidate, is also described. The invention includes the production of such enzymes and their use in an improved process for producing glucosone from glucose.