BREEDING METHOD OF BLUE MARKED TWO-LINE HYBRID WHEAT SYSTEM AND USE

    公开(公告)号:EP4342289A1

    公开(公告)日:2024-03-27

    申请号:EP22848488.7

    申请日:2022-07-25

    发明人: LI, Zhongan

    IPC分类号: A01H1/02 A01H1/04

    摘要: Disclosed in the present invention are a breeding method of a blue marked two-line hybrid wheat system and the use. According to the present invention, an exogenous translocation chromosome [T4AgL (a Ba-containing fragment)-4BL (a fragment close to a kinetochore).4thS and T4AgL (a Ba-containing fragment)-4BL (a fragment close to a kinetochore).4thS (a Rf-containing fragment)] that has a capability of completely recovering a recessive genic male sterile gene msl and contains a blue grain gene Ba exhibiting dose effect and xenia and an exogenous restoring gene Rf, and an exogenous translocation telomere [T4AgL (a Ba-containing fragment)-4thS (a Rf-containing fragment). T4thS (a Rf-containing fragment)-4AgL (a Ba-containing fragment) and T4thS (a Rf-containing fragment)-4AgL (a Ba-containing fragment)-4BL (a fragment close t0 a kinetochore).] are created. As a result, an improvement in the blue marked two-line hybrid wheat system is achieved.

    METHOD FOR PRODUCING TEMPERATURE-SENSITIVE MALE STERILE PLANT

    公开(公告)号:EP4129050A1

    公开(公告)日:2023-02-08

    申请号:EP21776678.1

    申请日:2021-03-25

    摘要: The present inventors aimed to identify the gene responsible for the temperature-sensitive male sterility trait in "PL12" and to provide a method for producing conditional male sterile plants targeting the gene, and performed whole genome sequence analysis on "PL12" and the original variety thereof to compare the two. As a result, it was found that there was a deletion of about 150 kb in "PL12" chromosome 7. Then, as for this region, six types of partial deletion lines were produced by genome editing, and the presence or absence of the temperature-sensitive male sterility trait in each line was used as an index to successfully narrow the range, containing the responsible mutation, down to about 10 kb. Furthermore, as a result of creating a line with genome editing-disrupted functions of the two genes located in this region, the gene responsible for the temperature-sensitive male sterility trait was successfully identified. Further, it was confirmed that the functions of the gene could be suppressed to impart the above trait to Arabidopsis thaliana and tomato, as in the case of rice.