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    PREPARATION OF TARGET PEPTIDES AND PROTEINS
    4.
    发明公开
    PREPARATION OF TARGET PEPTIDES AND PROTEINS 审中-公开

    公开(公告)号：EP4265636A1

    公开(公告)日：2023-10-25

    申请号：EP22168872.4

    申请日：2022-04-19

    申请人： mk2 Biotechnologies GmbH

    发明人： WIESBECK, Patrick ,  GIUMAN, Marco

    IPC分类号： C07K14/79 ,  C07K7/06 ,  C07K14/50

    摘要： The present invention relates to a fusion polypeptide comprising a purification domain; a cleavage domain; and a target peptide domain. The purification domain comprises 3 to 50 repeats of a nonapeptide, each nonapeptide independently having an amino acid sequence GX 2 X 3 X 4 X 5 X 6 X 7 X 8 X 9 , whereby X 2 may be G, N or D, X 3 may be A, S, G, D, E, L or N, X 4 may be G or A, X 5 may be N, D, A or S, X 6 may be D or N, X 7 may be T, I, V or L, X 8 may be L, V, I, F or Y, and X 9 may be Y, I, V, F, T, N, D, K or S. The cleavage domain comprises an autoprotease or an amino acid sequence X a SX b X c X d X e X f X g , whereby X a is only optional and may be any amino acid, X b may be H, R, S, N, Y, G, K or Q, X c may be H, S or N, X d may be T, S, L, M, G, N, Q or W, X e is only optional may be S, P, A, T, L, R or D, X f is only optional and may be L, P, E, G, T, A, F or S, and X g is only optional and may be any amino acid. Further disclosed is a method for preparing a target peptide using the fusion polypeptide of the invention.