公开(公告)号：JP2005053932A

公开(公告)日：2005-03-03

申请号：JP2004326629

申请日：2004-11-10

申请人： Brigham & Women's Hospital Inc ,  ザ・ブリガム・アンド・ウイメンズ・ホスピタル・インコーポレイテッドThe Brigham And Women′S Hospital, Inc.

发明人： YAO FENG

IPC分类号： A01K67/027 ,  A61K31/7052 ,  A61K31/7088 ,  A61K35/76 ,  A61K39/12 ,  A61K48/00 ,  A61P31/12 ,  A61P37/02 ,  C07K14/00 ,  C07K14/035 ,  C07K14/47 ,  C07K14/485 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/00 ,  C12N5/10 ,  C12N7/00 ,  C12N15/09 ,  C12N15/85 ,  C12N15/86 ,  C12P21/02

CPC分类号： C07K14/005 ,  A01K2217/05 ,  A61K48/00 ,  A61K2039/51 ,  C07K14/485 ,  C12N15/85 ,  C12N15/86 ,  C12N2710/16622 ,  C12N2710/16643 ,  C12N2830/00 ,  C12N2830/003

摘要： PROBLEM TO BE SOLVED: To provide many various compositions and methods, both having such common characteristics as to have gene expression regulated by a tet (tetracycline)-operator/repressor system. SOLUTION: A recombinant protein formed by host cells yielded by transfection by recombinationally treated viruses is provided. The host cell includes, in its genome, (a) a recombination promotor having a TATA element, (b) at least one tet-operator sequence starting in the range of from the last nucleotide of the TATA element to 6-nucleotide or at its downstream side, and (c) a gene which is at the 3' side of the operator and operably linked to the promotor and inhibits the replication of viruses when expressed. COPYRIGHT: (C)2005,JPO&NCIPI

摘要翻译： 要解决的问题：提供许多各种组合物和方法，它们都具有由tet（四环素） - 操作者/阻遏物系统调节的基因表达的共同特征。 解决方案：提供通过重组处理的病毒转染产生的宿主细胞形成的重组蛋白。 宿主细胞在其基因组中包含（a）具有TATA元件的重组启动子，（b）至少一个起始于TATA元件的最后一个核苷酸至6-核苷酸或其核苷酸的至少一个tet-操纵子序列 下游侧，和（c）处于操作者3'侧的基因，并且与启动子可操作地连接并在表达时抑制病毒的复制。 版权所有（C）2005，JPO＆NCIPI