AIR CLEANER
    2.
    发明专利

    公开(公告)号:JP2001187131A

    公开(公告)日:2001-07-10

    申请号:JP37312199

    申请日:1999-12-28

    摘要: PROBLEM TO BE SOLVED: To easily replace filters placed surrounding around an exciting light source. SOLUTION: An ultraviolet lamp 16 is supported in between the top plate 14 of an almost box shape light source housing part 11 and the bottom plate 15. On the filter face 17a of a square tube shape filter 17 formed of nonwoven fabric carrying titanium oxide, a slit 17b is formed along the longitudinal direction. The filter 17 can be mounted on a prescribed position by only pushing the filter 17 into the side plate 13 even in a condition the ultraviolet lamp 16 is mounted on and removed from the light source housing part 11 by only drawing out.

    BEEHIVE
    3.
    发明专利
    BEEHIVE 失效

    公开(公告)号:JP2001161205A

    公开(公告)日:2001-06-19

    申请号:JP34474299

    申请日:1999-12-03

    IPC分类号: A01K47/02 A01K47/06

    摘要: PROBLEM TO BE SOLVED: To provide a beehive suitable for bees for pollination due to ready transportation and installation and lightweight ness, cheapness, and the like. SOLUTION: This beehive is characterized in that the beehive is constituted of a case, a cover body and plural nest frames, a fixing mechanism for holding the nest frames is arranged in the inside of the case, openable and closable ventilation doors are installed at both right and left ends and a nest gate openable and closable in a sliding type is laid at one end face.

    FLOATING OIL REMOVING DEVICE
    4.
    发明专利

    公开(公告)号:JP2000070930A

    公开(公告)日:2000-03-07

    申请号:JP24989398

    申请日:1998-09-03

    IPC分类号: C02F1/40

    摘要: PROBLEM TO BE SOLVED: To improve the durability of a belt in a device in which the oil, waste liq., etc., floating on the surface of liq. is stuck on the belt and the stuck matter is removed at a scraping part. SOLUTION: Inner side surfaces of guide parts 21 and 22 of a driving roller 2 are constituted with inclined surface parts 21b and 22b. The belt engaged to the driving roller 2 is constituted from a stainless steel and its width W is set in longer than that of width d1 of a wheel periphery part 23 and in shorter than a distance d2 between the guide parts 21 and 22. The wheel periphery part 23 is constituted from an expanded metal.

    PURIFICATION OF LUCIFERASE
    6.
    发明专利

    公开(公告)号:JPH08238086A

    公开(公告)日:1996-09-17

    申请号:JP6884495

    申请日:1995-03-03

    发明人: SHIMAMURA TAMAO

    IPC分类号: C12N15/09 C12N9/00 C12N9/02

    摘要: PURPOSE: To purify in large quantities at low cost luciferase involving bioluminescence, useful as e.g. a clinical test agent, by subjecting a luciferase-contg. liquid to affinity chromatography using ATP, NAD or an analogous compound thereof. CONSTITUTION: A luciferase-contg. solution prepared from the body fluid or ground product of silkworm infected with the virus recombined with luciferase gene is put through a hydrophobic column with phenyl group as ligand; the resultant adsorbate is then eluted, and the eluate is purified by an affinity chromatography with adenosine triphosphate(ATP), nicotinamidadenine dinucleotide(NAD) or an analogous compound thereof, thus economically obtaining in large quantities the objective purified luciferase involving bioluminescence such as for firefly and usable as e.g. a clinical test agent by DNA probe method.

    METHOD FOR PURIFYING INFLUENZA HA PROTEIN

    公开(公告)号:JPH07258291A

    公开(公告)日:1995-10-09

    申请号:JP7777494

    申请日:1994-03-25

    摘要: PURPOSE:To obtain the subject high-purity protein useful as a vaccine having high safety and effectiveness by adsorbing an influenza HA protein-containing solution manifested by an insect cultured cell by using recombinant DNA technology on a specific saccharide molecule immobilized to a carrier and eluting. CONSTITUTION:A recombinant virus for manifesting an influenza HA protein integrated into a DNA encoding influenza HA protein is inoculated into an insect (e.g. silkworm larva) or an insect culture tissue to infect the insect, etc. On the fifth to the sixth day, the insect or the insect culture tissue is homogenized, centrifuged at high speed, precipitate is collected, dissolved in 10mM phosphoric acid buffer solution containing a surfactant, the solution is ultracentrifuged and a supernatant liquid is collected to give an influenza HA protein- containing solution. Then the solution is adsorbed on a compound comprising three or more saccharide molecules containing NeuAc alpha2, 3(6)Galbeta1, 3(4) as a free end immobilized to a carrier such as agarose gel, etc., and the adsorbed solution is eluted to give the objective purified influenza HA protein.

    VIRUS INFECTION FACTOR, VIRUS INFECTION-PROMOTIVE PROTEIN AND MEDIUM FOR CULTURING INSECT CELL CONTAINING THE SAME AND PRODUCTION OF USEFUL PROTEIN

    公开(公告)号:JPH07252298A

    公开(公告)日:1995-10-03

    申请号:JP30846894

    申请日:1994-11-18

    摘要: PURPOSE:To obtain a new substance capable of increasing the manifestation of useful substances through promotion of infecting insect cultured cells, etc., with recombinant virus, by heat treating silkworm body fluid followed by gel filtration thereof. CONSTITUTION:This virus infection factor is obtained by heat treating the body fluid taken from silkworm followed by gel filtration. This factor has the following characteristics: deactivating with trypsin or papain; adsorbable to cation exchange column; >=pH 9 in isoelectric point; and 10-50KDa in molecular weight. Specifically, this factor can be obtained by heat treatment of the body fluid taken from silkworm pref. at 60-70 deg.C for 30-60min followed by centrifugal separation of the fluid at 3000-5000rpm for 20-30min and then get filtration of the resultant supernatant using a gel such as Sephadex or Sepharose. It is recommended that the assay of this virus infection factor be conducted by a luminoassay technique using nuclear polyhedrosis virus recombined with the glowfly luciferase gene.

    GATE APPARATUS TO BE ATTACHED TO NEST BOX OF FLOWER-VISITING INSECT AND METHOD FOR TRANSPORTING AGENT OR POLLEN BY FLOWER-VISITING INSECT USING THE GATE APPARATUS

    公开(公告)号:JPH06169663A

    公开(公告)日:1994-06-21

    申请号:JP34986492

    申请日:1992-12-02

    IPC分类号: A01H1/02 A01K47/06

    摘要: PURPOSE:To provide a gate apparatus attached to a nest box of a flower-visiting insect and having a nest-outlet path and a nest-inlet path separated from each other to allow the insect to transport a drug or pollen for the crossing of a self-incompatible plant and free from phytotoxicity caused by the drug and to provide a method for allowing an insect to transport a drug or pollen. CONSTITUTION:The inside of a gate apparatus 1 is separated into a nest-outlet path 3 and a nest-inlet path 4 with a partition plate 2 perpendicular to a platform 9. A side wall 10 is perpendicularly bent toward the platform 9 and an end outlet port 6 of the nest outlet path 3 is formed between the partition plate 2 and the side wall 10. The outlet port 6 is positioned inside of the inlet port 8 of the nest inlet path 4. The box is covered with a removable lid 12 and an escape-prevention net 13 is attached to the reverse side of the lid 12. A lighting hole 14 is formed at an end of the side wall 11 near the end outlet port 6 of the nest-outlet path 3 and a drug-application sheet 11 is pasted on the bottom face and the inner wall face of the nest-outlet path 3. A drug or pollen is applied to the drug-application sheet 11 to apply the drug or pollen to an insect leaving the box through the nest-outlet path and allow the insect to transport the drug, etc.

    EUCARYOTIC CELL REARING PROMOTER AND CULTURING METHOD OF EUCARYOTIC CELL UTILIZING THE SAME PROMOTER

    公开(公告)号:JPH0479878A

    公开(公告)日:1992-03-13

    申请号:JP19305190

    申请日:1990-07-23

    发明人: IKETA KIYOSHI UNO JUN

    IPC分类号: C12N1/00 A61K35/64 A61K35/644

    摘要: PURPOSE:To obtain the title promoter capable of selectively rearing and promoting eucaryotic cell and eucaryotic microorganism by adding a hydrophilic solvent extract of propolis as an active ingredient. CONSTITUTION:Propolis, preferably grown in Brazil is as necessary preliminarily extracted with an organic solvent such as chloroform and then a hydrophilic solvent such as water or ethanol is added thereto and the propolis is subjected to extraction treatment at 20-30 deg.C for 24-48hr and filtered to provide the aimed promoter. Furthermore, eucaryotic microorganism of filamentous fungus such as the genus Saccharomyces and eucaryotic cell of animal cell of silkworm, etc. and plant cell of saffron, etc., are inoculated into a culture medium to which the promoter is added, preferably at an amount of 25-30g per l culture medium for cell culture to carry out culture.