Mass spectrometry method for simultaneous detection of metabolic enzyme activity and metabolite level
    9.
    发明专利
    Mass spectrometry method for simultaneous detection of metabolic enzyme activity and metabolite level 审中-公开
    用于同时检测代谢酶活性和代谢物水平的质谱方法

    公开(公告)号:JP2011167208A

    公开(公告)日:2011-09-01

    申请号:JP2011126859

    申请日:2011-06-07

    Inventor: CERDA BLAS

    CPC classification number: C12Q1/00 G01N33/6893 G01N2800/04

    Abstract: PROBLEM TO BE SOLVED: To provide a method for detecting a metabolic disorder in an individual.
    SOLUTION: The method for detecting the metabolic disorder, includes following steps: (a) contacting a sample containing (1) one or more metabolically indicative enzymes and (2) one or more metabolic analytes, with one or more substrates for the one or more enzymes to produce a reaction admixture, under conditions in which at least one of the enzymes can act on a corresponding substrate to generate at least one product; (b) contacting the reaction admixture with a reagent which inhibits the ability of the one or more enzymes to act on a corresponding substrate, in which the one or more metabolic analytes and the at least one product are soluble in the reagent, and to produce a test sample; and (c) determining the presence or amount of the one or more metabolic analytes and the at least one product contained in the test sample using mass spectrometry, in which a determined presence or amount of the one or more metabolic analytes and the at least one product correlates with presence or absence of the metabolic disorder.
    COPYRIGHT: (C)2011,JPO&INPIT

    Abstract translation: 待解决的问题:提供一种检测个体的代谢紊乱的方法。 检测代谢紊乱的方法包括以下步骤:(a)使含有(1)一种或多种代谢指示酶的样品和(2)一种或多种代谢分析物与一种或多种底物接触,用于 一种或多种酶以产生反应混合物,其中至少一种酶可以作用在相应的底物上以产生至少一种产物; (b)使反应混合物与抑制一种或多种酶作用于相应底物的能力的试剂接触,其中一种或多种代谢分析物和至少一种产物可溶于试剂中,并产生 测试样品 和(c)使用质谱法测定所述测试样品中所含的一种或多种代谢分析物和所述至少一种产物的存在或量,其中所述一种或多种代谢分析物和所述至少一种代谢分析物的确定存在或量 产物与代谢紊乱的存在或不存在相关。 版权所有(C)2011,JPO&INPIT

    Multipole ion guide interface for reduced background noise in mass spectrometry
    10.
    发明专利
    Multipole ion guide interface for reduced background noise in mass spectrometry 审中-公开
    用于减少大量光谱中背景噪声的多离子指南界面

    公开(公告)号:JP2014112570A

    公开(公告)日:2014-06-19

    申请号:JP2014064661

    申请日:2014-03-26

    CPC classification number: H01J49/04 H01J49/063

    Abstract: PROBLEM TO BE SOLVED: To provide an apparatus in which a multipole ion guide is configured to efficiently transport ions through multiple vacuum stages, while preventing background particles produced both in an ion source and along an ion transport pathway from reaching a detector to thereby improve signal-to-noise in mass spectra.SOLUTION: The RF multipole ion guide extends in a multiple vacuum pumping stage vacuum system continuously through one or more vacuum partitions between an upstream region (farther from the mass analyzer detector) of higher gas pressure and a downstream region of lower gas pressure. The ion guide is configured with an axis curved with respect to the direction of an ion beam and to prevent any line of sight between an upstream ion source region, as well as any or all higher pressure regions in which collisions between ions and background gas molecules occur, and the mass analyzer detector.

    Abstract translation: 要解决的问题:提供一种设备,其中多极离子导向器被配置为有效地将离子传输通过多个真空级,同时防止离子源和离子传输路径两者都产生的​​背景颗粒到达检测器,从而改善信号 噪声在质谱中。解决方案:RF多极离子导向器在多重真空泵级真空系统中连续地通过一个或多个真空隔板在较高气体压力的上游区域(更远离质量分析仪检测器)和下游 较低气压区域。 离子导向器配置有相对于离子束方向弯曲的轴线,并且防止上游离子源区域以及任何或所有较高压力区域之间的任何视线,其中离子和背景气体分子之间的碰撞 发生,质量分析仪检测器。

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