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    細胞培養装置
    1.
    发明专利
    細胞培養装置 有权

    公开(公告)号：JPWO2015068450A1

    公开(公告)日：2017-03-09

    申请号：JP2015546319

    申请日：2014-09-02

    申请人： 株式会社日立ハイテクノロジーズ

    发明人： 島瀬　明大 ,  明大 島瀬 ,  今井　一成 ,  一成 今井 ,  英一郎 高田 ,  英一郎 高田 ,  定光 麻生 ,  定光 麻生

    IPC分类号： C12M1/00

    CPC分类号： C12M23/58 ,  C12M23/40 ,  C12M29/00

    摘要： コストを抑えつつ、複数の培養容器間の均等・均質な送液を可能とする細胞培養装置である。本発明の細胞培養装置は、流体の導入口と排出口とを持つ密閉系の培養容器1が複数あり、複数の培養容器1が並列に接続され、1つの閉鎖培養系が形成されている細胞培養装置である。前記細胞培養装置は、複数の各培養容器1に接続された複数の流路を切替える1つの流路切替え機構8を有する。前記流路切替え機構8は、各培養容器1に分岐して接続された各々の個別流路のうち、1つの個別流路の流路抵抗を、残りの個別流路の流路抵抗よりも小さくする。前記流路切替え機構8は、1つの流路切替え部材を閉鎖培養系内に配置し、流路切替え部材の向きおよび位置の少なくとも一方を変えることで、流路抵抗の小さな個別流路を形成する。

    Nucleic acid analysis device, nucleic acid analysis device, and a device manufacturing method for nucleic acid analysis
    3.
    发明专利
    Nucleic acid analysis device, nucleic acid analysis device, and a device manufacturing method for nucleic acid analysis 有权

    公开(公告)号：JP5309092B2

    公开(公告)日：2013-10-09

    申请号：JP2010162403

    申请日：2010-07-20

    申请人： 株式会社日立ハイテクノロジーズ

    发明人： 俊郎 斎藤 ,  一成 今井

    IPC分类号： C12N15/09 ,  C12M1/00 ,  G01N21/64 ,  G01N33/553 ,  G01N37/00

    摘要： PROBLEM TO BE SOLVED: To improve the throughput of nucleic acid analysis by regularly arranging on a base plate fine particles on which nucleic acid synthetases and DNA probes capturing fragments of a nucleic acid sample are immobilized. SOLUTION: The device for analyzing the nucleic acids is obtained by previously immobilizing the nucleic acid synthetases, the DNA probesb, or the like on the fine particles, forming a metal pad pattern of gold or the like having diameters smaller than those of the fine particles on the base plate, and binding the fine particles with the pads through a chemical bond. When the fine particles have surface charges, the metal pad pattern of the gold or the like having diameters equal to or larger than the diameters of the fine particles is formed on the base plate, and the fine particles are connected with the pads through the chemical bond. As a result, the nucleic acid sample can be analyzed in the high throughput because many kinds of samples of the nucleic acid fragments can be immobilized on the base plate by being regularly arranged in high density. COPYRIGHT: (C)2012,JPO&INPIT

    Nucleic acid analysis device, and nucleic acid analysis apparatus
    10.
    发明专利
    Nucleic acid analysis device, and nucleic acid analysis apparatus 有权

    公开(公告)号：JP5260339B2

    公开(公告)日：2013-08-14

    申请号：JP2009018936

    申请日：2009-01-30

    申请人： 株式会社日立ハイテクノロジーズ

    发明人： 俊郎 齋藤 ,  一成 今井

    IPC分类号： C12N15/09 ,  C12M1/00 ,  G01N21/64 ,  G01N33/53 ,  G01N33/543 ,  G01N37/00

    CPC分类号： C12Q1/6816 ,  C12Q2565/50 ,  C12Q2563/143

    摘要： An object of the present invention is to regularly align microparticles, on each of which a nucleic acid synthetase or a DNA probe capable of capturing a nucleic acid sample fragment is immobilized, on a support so as to improve throughput of nucleic acid analysis. The present invention relates to a method comprising immobilizing a nucleic acid synthetase, a DNA probe, or the like in advance to a microparticle, forming a pattern of metal pads each having a diameter smaller than the microparticle diameter with gold or the like on a support, and allowing a microparticle to be bound to the pads via a chemical bond. In addition, when the surfaces of microparticles are electrically charged, a pattern of metal pads each having a diameter equivalent to or larger than the microparticle diameter is formed with gold or the like on a support and a microparticle is allowed to be bound to the pads via a chemical bond. According to the present invention, many types of nucleic acid fragment samples can be regularly aligned at a high density and immobilized on a support. This allows high throughput analysis of nucleic acid samples. For example, if microparticles are immobilized at 1-micron pitches, a high density of 106 nucleic acid fragments/emm2 can be readily achieved.