公开(公告)号：JP4099898B2

公开(公告)日：2008-06-11

申请号：JP12702599

申请日：1999-05-07

申请人： 王子製紙株式会社

发明人： 敬悟 土肥 ,  哲 河津 ,  啓子 近藤

IPC分类号： A01H1/00 ,  C12N15/09 ,  A01H4/00 ,  C12N5/10 ,  C12N15/82 ,  C12N15/84

CPC分类号： C12N15/8205 ,  A01H4/005

摘要： The present invention discloses a process for transforming mature trees of Eucalyptus plants comprising: inducing adventitious shoots from segments of the explant obtained from an adult tree of a Eucalyptus plant, preculturing the adventitious shoots in infection induction medium, infecting the adventitious shoots subjected to infection induction treatment with infection medium containing Agrobacterium tumefaciens, and rotary-culturing the infected explant segments in sterilization medium containing antibiotic; whereby sterilizing and forming transgenic calli, which regenerate transgenic plants by way of formation of shoot primordia by rotary-culturing under illumination.