How to transform a mature tree of Eucalyptus plant

    公开(公告)号:JP4099898B2

    公开(公告)日:2008-06-11

    申请号:JP12702599

    申请日:1999-05-07

    CPC分类号: C12N15/8205 A01H4/005

    摘要: The present invention discloses a process for transforming mature trees of Eucalyptus plants comprising: inducing adventitious shoots from segments of the explant obtained from an adult tree of a Eucalyptus plant, preculturing the adventitious shoots in infection induction medium, infecting the adventitious shoots subjected to infection induction treatment with infection medium containing Agrobacterium tumefaciens, and rotary-culturing the infected explant segments in sterilization medium containing antibiotic; whereby sterilizing and forming transgenic calli, which regenerate transgenic plants by way of formation of shoot primordia by rotary-culturing under illumination.

    IMPROVEMENT IN EFFICIENCY OF GENE TRANSFER TO PLANT CELL

    公开(公告)号:JP2000342256A

    公开(公告)日:2000-12-12

    申请号:JP15802599

    申请日:1999-06-04

    申请人: JAPAN TOBACCO INC

    摘要: PROBLEM TO BE SOLVED: To improve the efficiency of gene transfer to a plant cell simply carried out through a bacterium of the genus Agrobacterium without damaging a tissue, to perform transformation and to better a breed by centrifuging a plant cell or a plant tissue. SOLUTION: A plant cell or a plant tissue of rice plant, maize, etc., is centrifuged at 100-250,000 G, preferably 500-200,000 G, more preferably 1,000-150,000 G centrifugal acceleration for 1 second to 4 hours, preferably for 5 minutes to 2 hours to improve the efficiency of gene transfer to a plant cell carried out through a bacterium of the genus Agrobacterium. Preferably the plant cell or plant tissue is derived from an angiosperm, a monocotyledon or a gramineous plant. Preferably after the plant cell or plant tissue is centrifuged, a gene transfer treatment is performed.

    TRANSGENIC PLANT BELONGING TO SPECIES CUCUMIS MELO

    公开(公告)号:JP2000300098A

    公开(公告)日:2000-10-31

    申请号:JP32098199

    申请日:1999-11-11

    申请人: BIOCEM

    摘要: PROBLEM TO BE SOLVED: To obtain a transgenic plant capable of being regenerated from a transformed tissue in the species Cucumis melo and excellent in virus resistance by including a DNA sequence transduced through mediation of Agrobacterium tumefaciens. SOLUTION: This transgenic plant is a transgenic plant of the normal phenotype belonging to the species Cucumis melo and contains one or more DNA sequences transduced through mediation of Agrobacterium tumefaciens. Further, the transgenic plant of the normal phenotype belonging to the species Cucumis melo is preferably prepared by including one or more DNA sequences capable of imparting resistance to cucumber mosaic and having an encoding sequence represented by the formula or capable of encoding a capsid protein of the cucumber mosaic virus. The plant tissue is preferably a melon and the tissue is preferably a cotyledon obtained from an embryo germinated for 0-4 days or a shoot bud.