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1.发明专利
公开(公告)号：JP2012183071A
公开(公告)日：2012-09-27
申请号：JP2012122888
申请日：2012-05-30
发明人： LUKYANOV SERGEY ANATOLIEVICH , FRADKOV ARCADY FEDOROVICH , LABAS YULII ALEKSANDROVICH , MATZ MIKHAIL VLADIMIROVICH , TERSKIKH ALEXEY
CPC分类号： C07K14/435 , C07K2319/60
摘要： PROBLEM TO BE SOLVED: To provide anthozoa-derived chromo/fluoroproteins and mutants thereof, as well as nucleic acid compositions encoding the same.SOLUTION: Specific proteins of interest include chromo/fluoroproteins from the following specific anthozoa species: Anemonia majano, Clavularia sp., Zoanthus sp., Discosoma striata, Discosoma sp. "red", Anemonia sulcata, Discosoma sp. "green", Discosoma sp. "magenta", and mutants thereof. Fragments of the subject proteins and nucleic acids encoding the same, as well as antibodies to the subject proteins and transgenic cells and organisms are also provided. Kits for use in such applications that include the subject proteins are provided.
摘要翻译： 待解决的问题：提供来源于其的染色体/荧光素蛋白及其突变体，以及编码其的核酸组合物。 感兴趣的特异性蛋白质包括来自以下特异性anthozoa物种的色素/荧光蛋白：Anemonia majano，Clavularia sp。，Zoanthus sp。，Discosoma striata，Discosoma sp。 “红”，海棠，盘豆属 “绿色”，Discosoma sp。 “洋红色”及其突变体。 还提供了主题蛋白和编码它们的核酸的片段，以及针对受试者蛋白质和转基因细胞和生物体的抗体。 提供了在包括主题蛋白质的这些应用中使用的试剂盒。 版权所有（C）2012，JPO＆INPIT
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2.发明专利Fluorescent protein from non-bioluminescent species of class anthozoa, gene encoding such protein and use thereof 有权来自非生物发光物质的类别ANTHOZOA，编码这种蛋白质的基因的荧光蛋白及其用途
公开(公告)号：JP2010268798A
公开(公告)日：2010-12-02
申请号：JP2010141050
申请日：2010-06-21
发明人： LUKYANOY SERGEY ANATOLIEVICH , FRADKOV ARCADY FEDOROVICH , LABAS YULII ALEKSANDROVICH , MATZ MIKHAIL VLADIMIROVICH
IPC分类号： C12N15/09 , C12Q1/68 , C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P21/02 , C12Q1/02 , G01N21/78 , G01N33/48 , G01N33/53
CPC分类号： C07K14/43595 , C07K14/43504
摘要： PROBLEM TO BE SOLVED: To provide new fluorescent proteins, to provide a method for identifying DNA sequence encoding the proteins, and to provide the use thereof.
SOLUTION: There are provided new fluorescent proteins (specifically isolated and purified luminescent proteins selected from the group consisting of amFP486, cFP484, zFP506, zFP538, dsFP483, drFP583, asFP600, dgFP512 and dmFP592) from non-bioluminescent organisms from the Class Anthozoa. The method identifying the nucleic acid sequence encoding the fluorescent proteins and further a method for analyzing the proteins are provided.
COPYRIGHT: (C)2011,JPO&INPIT摘要翻译： 要解决的问题：提供新的荧光蛋白，以提供用于鉴定编码蛋白质的DNA序列的方法，并提供其用途。 解决方案：从Class的非生物发光生物体中提供新的荧光蛋白（特别是分离和纯化的发光蛋白，其选自amFP486，cFP484，zFP506，zFP538，dsFP483，drFP583，asFP600，dgFP512和dmFP592） 珊瑚虫。 提供了鉴定编码荧光蛋白的核酸序列的方法以及进一步分析蛋白质的方法。 版权所有（C）2011，JPO＆INPIT
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