公开(公告)号：JP2010046080A

公开(公告)日：2010-03-04

申请号：JP2009241780

申请日：2009-10-20

Applicant: Koninkl Dsm Nv ,  コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ

Inventor： VAN DEN BRINK JOHANNES MAARTEN ,  SELTEN GERARD CORNELIS MARIA ,  VAN DEN HOMBERGH JOHANNES PETRUS THEODORUS WILHELM

IPC: C12N15/09 ,  C12N1/15 ,  C12N9/16 ,  C12N9/24 ,  C12N15/10 ,  C12N15/80 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/66 ,  C12R1/685

CPC classification number: C12N15/1086 ,  C12N15/1034 ,  C12N15/80

Abstract: PROBLEM TO BE SOLVED: To provide a method for isolating a DNA sequence encoding protein(s) having properties of interest by means of expression cloning in filamentous fungal host cells.
SOLUTION: The method comprises (a) a step of preparing a DNA library as an appropriate cloning vector from an organism supposed to have the ability to produce one or more proteins having properties of interest, (b) a step of transforming filamentous host cells by the DNA library, (c) a step of culturing transformed cells obtained in the step (c) under the condition of inducing the expression of the DNA sequence of the DNA library, and (d) a step of screening the transformed cell clone expressing the protein (s) having properties of interest by analysis of the protein (s) produced in the step (c).
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 待解决的问题：提供通过在丝状真菌宿主细胞中的表达克隆分离编码具有感兴趣性质的蛋白质的DNA序列的方法。 解决方案：该方法包括（a）从被认为具有产生一种或多种具有感兴趣性质的蛋白质的能力的生物体制备作为合适的克隆载体的DNA文库的步骤，（b）转化丝状的步骤 宿主细胞，（c）在诱导DNA文库的DNA序列表达的条件下培养在步骤（c）中获得的转化细胞的步骤，和（d）筛选转化细胞的步骤 通过分析步骤（c）中产生的蛋白质来表达具有感兴趣性质的蛋白质的克隆。 版权所有（C）2010，JPO＆INPIT