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公开(公告)号:JP6246953B2
公开(公告)日:2017-12-13
申请号:JP2016571827
申请日:2015-12-25
申请人: キッコーマン株式会社
CPC分类号: C12P13/04 , C12N15/09 , C12N9/10 , C12N9/1007 , C12N9/13 , C12Y201/01 , C12Y208/01007 , C12R1/66 , C12R1/665 , C12R1/685 , C12R1/69
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公开(公告)号:JP4809905B2
公开(公告)日:2011-11-09
申请号:JP2009049052
申请日:2009-03-03
发明人: ジユ,ダイ , レイシュア,リンダ・エル
摘要: The invention includes isolated polynucleotide molecules that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention includes a method of enhancing a bioprocess utilizing a fungus. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to a promoter. The polynucleotide sequence is expressed to promote a first morphology. The first morphology of the transformed fungus enhances a bioprocess relative to the bioprocess utilizing a second morphology.
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公开(公告)号:JP2010521188A
公开(公告)日:2010-06-24
申请号:JP2009554017
申请日:2008-03-18
IPC分类号: C12N9/06 , A23J1/20 , A23K1/16 , A23K1/165 , C12N15/09 , C12P21/04 , C12Q1/26 , C12Q1/68 , C12R1/685
CPC分类号: A23J3/08 , A23J3/14 , A23J3/22 , A23L7/107 , A23L29/06 , C12N9/0022 , C12Y104/03013
摘要: 本発明は、O
2 からH
2 O
2 への還元が続いて起こる、タンパク質またはペプチド中のリシル基の対応するアルデヒドへの酸化的脱アミノ化を触媒できる、単離されたリシルオキシダーゼに関し、リシルオキシダーゼは、(a)37℃およびpH7.0における活性判定で、少なくとも1.1、好ましくは少なくとも1.3、より好ましくは少なくとも1.4のAc−Gly−Lys−OMeに対するリシルオキシダーゼ活性とベンジルアミンに対するリシルオキシダーゼ活性との比率を示し、(b)0〜60℃の温度で最適活性を有する。
【選択図】図1-
公开(公告)号:JP2010046080A
公开(公告)日:2010-03-04
申请号:JP2009241780
申请日:2009-10-20
发明人: VAN DEN BRINK JOHANNES MAARTEN , SELTEN GERARD CORNELIS MARIA , VAN DEN HOMBERGH JOHANNES PETRUS THEODORUS WILHELM
IPC分类号: C12N15/09 , C12N1/15 , C12N9/16 , C12N9/24 , C12N15/10 , C12N15/80 , C12P21/02 , C12Q1/68 , C12R1/66 , C12R1/685
CPC分类号: C12N15/1086 , C12N15/1034 , C12N15/80
摘要: PROBLEM TO BE SOLVED: To provide a method for isolating a DNA sequence encoding protein(s) having properties of interest by means of expression cloning in filamentous fungal host cells.
SOLUTION: The method comprises (a) a step of preparing a DNA library as an appropriate cloning vector from an organism supposed to have the ability to produce one or more proteins having properties of interest, (b) a step of transforming filamentous host cells by the DNA library, (c) a step of culturing transformed cells obtained in the step (c) under the condition of inducing the expression of the DNA sequence of the DNA library, and (d) a step of screening the transformed cell clone expressing the protein (s) having properties of interest by analysis of the protein (s) produced in the step (c).
COPYRIGHT: (C)2010,JPO&INPIT摘要翻译: 待解决的问题:提供通过在丝状真菌宿主细胞中的表达克隆分离编码具有感兴趣性质的蛋白质的DNA序列的方法。 解决方案:该方法包括(a)从被认为具有产生一种或多种具有感兴趣性质的蛋白质的能力的生物体制备作为合适的克隆载体的DNA文库的步骤,(b)转化丝状的步骤 宿主细胞,(c)在诱导DNA文库的DNA序列表达的条件下培养在步骤(c)中获得的转化细胞的步骤,和(d)筛选转化细胞的步骤 通过分析步骤(c)中产生的蛋白质来表达具有感兴趣性质的蛋白质的克隆。 版权所有(C)2010,JPO&INPIT
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公开(公告)号:JP4425354B2
公开(公告)日:2010-03-03
申请号:JP54827598
申请日:1998-05-01
发明人: エイドレビッチ,リリアナ , ソー チョイ,ジョン , ジェイ. モリソン,クリスティーン , レイス,エロル
CPC分类号: C12Q1/6895
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公开(公告)号:JP4339400B2
公开(公告)日:2009-10-07
申请号:JP50375197
申请日:1996-06-24
IPC分类号: C12N15/09 , C07K14/38 , C07K14/47 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/04 , C12N9/24 , C12N9/88 , C12N15/10 , C12N15/31 , C12N15/63 , C12N15/65 , C12N15/80 , C12Q1/02 , C12Q1/68 , C12R1/225 , C12R1/645 , C12R1/66 , C12R1/665 , C12R1/685 , C12R1/69 , C12R1/77 , C12R1/80 , C12R1/85 , C12R1/865
CPC分类号: C12N15/635 , C07K14/38 , C07K14/4705 , C12N9/0006 , C12N9/2434 , C12N9/248 , C12N9/88 , C12N15/1027 , C12N15/63 , C12N15/65 , C12N15/80 , C12Q1/02 , C12Y302/01003 , C12Y302/01008 , C12Y302/01015 , C12Y302/01037 , C12Y302/01055 , C12Y302/01099
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