公开(公告)号：US5004805A

公开(公告)日：1991-04-02

申请号：US58211

申请日：1987-06-04

申请人： Eiichi Gohda ,  Hirohito Tsubouchi ,  Hiroyuki Nakayama ,  Shuichi Hirono ,  Kozo Takahashi ,  Shuji Hashimoto ,  Yasushi Daikuhara

发明人： Eiichi Gohda ,  Hirohito Tsubouchi ,  Hiroyuki Nakayama ,  Shuichi Hirono ,  Kozo Takahashi ,  Shuji Hashimoto ,  Yasushi Daikuhara

IPC分类号： A61K38/22 ,  A61K35/16 ,  A61K38/00 ,  A61P1/16 ,  C07K14/475

CPC分类号： C07K14/4753 ,  A61K38/00 ,  Y10S530/846

摘要： A hepatocyte growth factor comprising a proteinous substance derived from human blood and showing the following physicochemical properties and physiologic activity:(i) its molecular weight is estimated to be approximately 76,000 to 92,00 by SDS-PAGE analysis (under non-reductive conditon);(ii) it possesses HGF activity;(iii) the HGF activity is inactivated by heat treatment for 10 minutes at 80.degree. C.;(iv) the HGF activity may be lost by digestion with trypsin or with chymotrypsin; and(v) it has a high affinity for heparin.

摘要翻译： 包含衍生自人血液的蛋白质物质并显示以下物理化学性质和生理活性的肝细胞生长因子：（i）通过SDS-PAGE分析估计其分子量为约76,000至92,00（在非还原条件下） ; （ii）具有HGF活性; （iii）通过在80℃热处理10分钟使HGF活性失活。 （iv）用胰蛋白酶消化或用胰凝乳蛋白酶消失HGF活性; 和（v）它对肝素具有高亲和力。