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1.发明授权Process for oxidation of steroids and genetically engineered cells used therein 失效用于其中使用的类固醇和基因工程细胞的氧化过程公开(公告)号：US06171836B2
公开(公告)日：2001-01-09
申请号：US09099011
申请日：1998-06-17
IPC分类号： C12N902
CPC分类号： C12Y106/02004 , C12N9/0006 , C12N9/0042 , C12N9/0071 , C12N9/0095 , C12P33/00 , C12Y101/01053 , C12Y114/15004 , C12Y114/15006 , C12Y114/99009 , C12Y118/01002
摘要： An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17&agr;-hydroxyprogesterone; the conversion of 17&agr;-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host.
摘要翻译： 可在重组宿主中操作的表达盒，其包含编码蛋白质的异源DNA编码序列，所述蛋白质单独或与一种或多种另外的蛋白质协同作用来催化用于将胆固醇转化为氢化可的松的生物学途径中的氧化步骤 该步骤选自：将胆固醇转化为孕烯醇酮;将孕烯醇酮转化为孕酮;将孕酮转化为17α-羟孕酮;将17α-羟孕酮转化为皮质醇;将皮质醇转化为氢化可的松， 并且相应的控制序列在所述主机中有效。
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公开(公告)号：US5869283A
公开(公告)日：1999-02-09
申请号：US418085
申请日：1995-04-06
CPC分类号： C12Y106/02004 , C12N9/0006 , C12N9/0042 , C12N9/0071 , C12N9/0095 , C12P33/00 , C12Y101/01053 , C12Y114/15004 , C12Y114/15006 , C12Y114/99009 , C12Y118/01002
摘要： An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of:the conversion of cholesterol to pregnenolone;the conversion of pregnenolone to progesterone;the conversion of progesterone to 17.alpha.-hydroxy-progesterone;the conversion of 17.alpha.-hydroxyprogesterone to cortexolone;the conversion of cortexolone to hydrocortisone, and thecorresponding control sequences effective in said host.
摘要翻译： 可在重组宿主中操作的表达盒，其包含编码蛋白质的异源DNA编码序列，所述蛋白质单独或与一种或多种另外的蛋白质协同作用来催化用于将胆固醇转化为氢化可的松的生物学途径中的氧化步骤 该步骤选自：胆固醇转化为孕烯醇酮; 孕烯醇酮转化为孕酮; 孕酮转化为17α-羟基孕酮; 将17α-羟基孕酮转化为皮质醇; 将皮质醇转化为氢化可的松，以及相应的控制顺序在所述宿主中有效。
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3.发明授权Process for oxidation of steroids and genetically engineered cells used therein 失效用于其中使用的类固醇和基因工程细胞的氧化过程公开(公告)号：US06632633B1
公开(公告)日：2003-10-14
申请号：US09098877
申请日：1998-06-17
IPC分类号： C12N1509
CPC分类号： C12Y106/02004 , C12N9/0006 , C12N9/0042 , C12N9/0071 , C12N9/0095 , C12P33/00 , C12Y101/01053 , C12Y114/15004 , C12Y114/15006 , C12Y114/99009 , C12Y118/01002
摘要： An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins of catalyzing an oxidation step in the biological pathway, for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17&agr;-hydroxyprogesterone; the conversion of 17&agr;-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in that host.
摘要翻译： 可在重组宿主中操作的表达盒，其包含编码蛋白质的异源DNA编码序列，其单独或与一种或多种催化生物学途径中的氧化步骤的其它蛋白质协同作用，用于将胆固醇转化为氢化可的松 ，其步骤选自：胆固醇转化为孕烯雌酚; 将孕烯醇酮转化为孕酮;将孕酮转化为17α-羟孕酮; 将17α-羟孕酮转化为皮质醇;将皮质醇转化为氢化可的松，以及相应的对照序列在该宿主中有效。
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