公开(公告)号：US20240352494A1

公开(公告)日：2024-10-24

申请号：US18683552

申请日：2022-08-15

Applicant: ADAMA CHILE SA

Inventor： Vincent F. CATALDO ,  Felipe VAREA ,  Andrés ARIZTÍA ,  Juan Manuel PUIG ,  Eduardo Esteban Agosin TRUMPER

IPC: C12P17/18 ,  C07D473/34 ,  C07H19/167 ,  C12N9/02 ,  C12N9/10 ,  C12N9/24

CPC classification number: C12P17/182 ,  C07D473/34 ,  C07H19/167 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/1085 ,  C12N9/2497 ,  C12Y106/02004 ,  C12Y205/01

Abstract: Metabolically-engineered yeast strains are provided. such as metabolically-engineered Saccharomyces cerevisiaestrains. producing high amounts of at least one. preferably all four natural cytokinins: trans-zeatin (tZ), trans-zeatin riboside (tZR). isopentenyladenine (iP) and isopenteny ladenine riboside (iPR).

公开(公告)号：US12065685B1

公开(公告)日：2024-08-20

申请号：US18368369

申请日：2023-09-14

Applicant: AMYRIS, INC.

Inventor： Sean Lund ,  Gale Wichmann

IPC: C12P19/56 ,  C12N15/81

CPC classification number: C12P19/56 ,  C12N15/81 ,  C12Y106/02004 ,  C12Y114/14 ,  C12Y205/01029 ,  C12Y402/03019 ,  C12Y505/01012

Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of UGT76G1. The host cell further contains one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of rebaudioside M.

公开(公告)号：US20170275598A1

公开(公告)日：2017-09-28

申请号：US15619176

申请日：2017-06-09

Applicant: Codexis, Inc.

Inventor： Robert Osborne ,  Vesna Mitchell ,  Khin Yu Naing Htwe ,  Xiyun Zhang ,  Erika M. Milczek ,  Jeffrey C. Moore

IPC: C12N9/02

CPC classification number: C12N9/0071 ,  C07K14/80 ,  C12N9/0042 ,  C12P13/001 ,  C12Y106/02004 ,  C12Y114/14001

Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

公开(公告)号：US20170247725A1

公开(公告)日：2017-08-31

申请号：US15278561

申请日：2016-09-28

Applicant: California Institute of Technology

Inventor： Pedro S. Coelho ,  Eric M. Brustad ,  Frances H. Arnold ,  Zhan Wang ,  Jared C. Lewis

IPC: C12P13/02 ,  C12N9/02 ,  C12P7/62

CPC classification number: C12P13/02 ,  C12N9/0004 ,  C12N9/0042 ,  C12P7/62 ,  C12Y106/02004 ,  Y02P20/52

Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.

公开(公告)号：US20170226122A1

公开(公告)日：2017-08-10

申请号：US15492180

申请日：2017-04-20

Applicant: University of Rochester

Inventor： Rudi Fasan ,  Kai-Dong Zhang

IPC: C07D493/18 ,  C12P17/18 ,  C12N9/02

CPC classification number: C07D493/18 ,  C07D491/18 ,  C12N9/0042 ,  C12N9/0071 ,  C12P17/181 ,  C12Y106/02004 ,  C12Y114/00

Abstract: Derivatives of the antimalarial agent artemisinin, compositions comprising the derivatives, methods for preparing the derivatives, and their uses in pharmaceutical compositions intended for the treatment of parasitic infections are provided. Methods are provided for the production of artemisinin derivatives via functionalization of positions C7 and C6a, and optionally, in conjunction with modifications at positions C10 and C9, via chemoenzymatic methods. Recombinant cytochrome P450 polypeptides are also provided for use in the methods. The artemisinin derivatives can be used for the treatment of malaria and other parasitic infections, alone or in combination with other antiparasitic drugs.

公开(公告)号：US09708587B2

公开(公告)日：2017-07-18

申请号：US14794249

申请日：2015-07-08

Applicant: Codexis, Inc.

Inventor： Robert Osborne ,  Vesna Mitchell ,  Khin Yu Naing Htwe ,  Xiyun Zhang ,  Erika M. Milczek ,  Jeffrey C. Moore

IPC: C12N9/02

CPC classification number: C12N9/0071 ,  C12N9/0042 ,  C12Y106/02004 ,  C12Y114/14001

Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

公开(公告)号：US20170183689A1

公开(公告)日：2017-06-29

申请号：US15224900

申请日：2016-08-01

Applicant: The California Institute of Technology

Inventor： Frances Arnold ,  Peter Meinhold ,  Matthew W. Peters ,  Rudi Fasan ,  Mike M.Y. Chen

IPC: C12P7/04 ,  C12P7/06 ,  C12N9/02

CPC classification number: C12P7/04 ,  C07K14/32 ,  C12N9/0042 ,  C12N9/0071 ,  C12N9/0077 ,  C12P7/06 ,  C12Y106/02004 ,  C12Y114/14001 ,  C12Y114/15 ,  C12Y114/15003 ,  Y02P20/52

Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.

公开(公告)号：US09683220B2

公开(公告)日：2017-06-20

申请号：US15171116

申请日：2016-06-02

Applicant: Codexis, Inc.

Inventor： Robert Osborne ,  Vesna Mitchell ,  Khin Yu Naing Htwe ,  Xiyun Zhang ,  Erika M. Milczek ,  Jeffrey C. Moore

IPC: C12N9/02 ,  C12N15/52 ,  C12N1/16 ,  C12N1/20 ,  C12N15/63

CPC classification number: C12N9/0071 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0042 ,  C12N15/52 ,  C12N15/63 ,  C12Y106/02004 ,  C12Y114/14001

Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

公开(公告)号：US09663532B2

公开(公告)日：2017-05-30

申请号：US14438833

申请日：2013-10-28

Applicant: University of Rochester

Inventor： Rudi Fasan ,  Kai-Dong Zhang

IPC: C07D491/18 ,  C07D493/18 ,  C12N9/00 ,  C12N9/02

CPC classification number: C07D493/18 ,  C07D491/18 ,  C12N9/0042 ,  C12N9/0071 ,  C12P17/181 ,  C12Y106/02004 ,  C12Y114/00

Abstract: Derivatives of the antimalarial agent artemisinin, compositions comprising the derivatives, methods for preparing the derivatives, and their uses in pharmaceutical compositions intended for the treatment of parasitic infections are provided. Methods are provided for the production of artemisinin derivatives via functionalization of positions C7 and C6a, and optionally, in conjunction with modifications at positions C10 and C9, via chemoenzymatic methods. Recombinant cytochrome P450 polypeptides are also provided for use in the methods. The artemisinin derivatives can be used for the treatment of malaria and other parasitic infections, alone or in combination with other antiparasitic drugs.

公开(公告)号：US20170009213A1

公开(公告)日：2017-01-12

申请号：US15171116

申请日：2016-06-02

Applicant: Codexis, Inc.

Inventor： Robert Osborne ,  Vesna Mitchell ,  Khin Yu Naing Htwe ,  Xiyun Zhang ,  Erika M. Milczek ,  Jeffrey C. Moore

IPC: C12N9/02

CPC classification number: C12N9/0071 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0042 ,  C12N15/52 ,  C12N15/63 ,  C12Y106/02004 ,  C12Y114/14001

Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

Abstract translation: 本发明提供了改进的活性改善的P450-BM3变体。 在一些实施方案中，P450-BM3变体在宽范围的底物上表现出改善的活性。