公开(公告)号：US20060105402A1

公开(公告)日：2006-05-18

申请号：US11272971

申请日：2005-11-15

申请人： Gennady Rott ,  Fred Samuels ,  Falk Fish ,  Ariela Ben-Zvi

发明人： Gennady Rott ,  Fred Samuels ,  Falk Fish ,  Ariela Ben-Zvi

IPC分类号： G01N33/567 ,  C12Q1/56 ,  C12M1/34

CPC分类号： G01N33/543 ,  G01N33/5302 ,  G01N33/54313 ,  G01N33/80

摘要： A method for the detection and/or visualization of particle agglutination in a particle suspension is provided, comprising placing a volume of the particle suspension and a volume of a solution or suspension containing an agglutinating agent at substantially the same selected location on a surface of a filter constructed so as to permit passage of individual unagglutinated particles in a direction perpendicular to the surface. A wash solution at optionally placed at substantially the same location as that of said agglutinating agent, and the surface is observed for the presence of particles. There is also provided a method for detection of agglutination reactions, such as used in blood grouping, reverse grouping and cross-matching. A device and a kit based on the invention is also claimed, which facilitates blood grouping, reverse grouping and matching in non-laboratory environment without the need for laboratory instruments.

摘要翻译： 提供了用于检测和/或可视化颗粒悬浮液中的颗粒凝集的方法，包括将体积的颗粒悬浮液和一定体积的含有凝集剂的溶液或悬浮液放置在基本上相同的选定位置的 过滤器被构造成允许单个未凝集的颗粒在垂直于表面的方向上通过。 任选地在与所述凝集剂的位置基本上相同的位置处的洗涤溶液，并观察表面存在颗粒。 还提供了用于血液分组，反向分组和交叉匹配中使用的凝集反应检测方法。 还要求保护基于本发明的装置和试剂盒，其不需要实验室仪器便于非实验室环境中的血液分组，反向分组和匹配。

公开(公告)号：US20050084879A1

公开(公告)日：2005-04-21

申请号：US10885688

申请日：2004-07-08

申请人： Gennady Rott ,  Fred Samuels ,  Falk Fish

发明人： Gennady Rott ,  Fred Samuels ,  Falk Fish

IPC分类号： C12Q1/68 ,  G01N33/543 ,  G01N33/80

CPC分类号： G01N33/80

摘要： A method for the detection and/or visualization of particle agglutination, comprising: placing a volume of a suspension of the particles on a filter, the filter being constructed so as to permit passage of individual particles; placing a volume of a solution or suspension containing an agglutinating agent at the location of the particle suspension; optionally placing a wash solution at the location of the agglutinating agent; and observing the surface at the location for the presence of particles, such presence indicating that agglutination of the particles occurred. There is also provided a method for detection of agglutination reactions in general and hemagglutination reactions, such as used in blood grouping and cross-matching, in particular. The method is comprised of successive vertical additions of whole blood, blood grouping reagent and wash to a filter. In case of hemagglutination a colored, preferably red or reddish dot becomes visible after washing. A device and a kit based on the invention is also claimed and facilitates blood grouping and matching in non-laboratory environment without the need for laboratory instruments.

摘要翻译： 一种用于检测和/或可视化颗粒凝集的方法，包括：将一定体积的所述颗粒的悬浮液放置在过滤器上，所述过滤器被构造成允许单个颗粒通过; 将一定体积的含有凝集剂的溶液或悬浮液放置在颗粒悬浮液的位置; 任选地将洗涤溶液置于凝集剂的位置; 并且在该位置处观察颗粒存在的表面，这种存在表明颗粒的凝集发生。 还提供了一般检测凝集反应的方法，特别是用于血液分组和交叉匹配的血细胞凝集反应。 该方法由连续垂直添加全血，血液分组试剂和洗涤组成。 在血细胞凝集的情况下，洗涤后可见有色的，优选的红色或微红色点。 还要求一种基于本发明的装置和试剂盒，并且便于非实验室环境中的血液分组和匹配，而不需要实验室仪器。