公开(公告)号：US20080113349A1

公开(公告)日：2008-05-15

申请号：US11592117

申请日：2006-11-03

申请人： Pranvera Ikonomi ,  Jason Cooper ,  Greg Sykes ,  Yvonne Reid

发明人： Pranvera Ikonomi ,  Jason Cooper ,  Greg Sykes ,  Yvonne Reid

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12Q1/00

CPC分类号： C12Q1/689 ,  C12Q1/6881 ,  C12Q2600/16

摘要： The present invention relates to a PCR (polymerase chain reaction) based assay that is useful for detecting, identifying, quantitating and analysis of a target nucleic acid (target nucleic acid hereinafter) in a sample. More specifically, the present invention relates to a PCR based assay that can improve accuracy in detecting, identifying, and quantitating contamination in mammalian cell lines using a PCR-based assay of nucleic acid oligonucleotides (oligoprobes) having a sequence expected to be complementary to a target nucleic acid sequence in a sample. More specifically, the sample may contain either cells or nucleic acid isolated from a cell line. The present invention also relates to a detection kit using the PCR-based assay. The invention also involves a method for using specifically produced nucleic acids complementary to specific sequences or populations of different sequences of the cytochrome c oxidase I (COI) and/or cytochrome b, to detect, identify, and quantify specific organisms, groups of organisms, groups of eukaryotic cells or viruses in cells.

摘要翻译： 本发明涉及可用于检测，鉴定，定量和分析样品中靶核酸（下文中的靶核酸）的基于PCR（聚合酶链式反应）的测定法。 更具体地，本发明涉及基于PCR的测定法，其可以使用具有期望与其互补的序列的核酸寡核苷酸（寡核苷酸）的基于PCR的测定来提高检测，鉴定和定量哺乳动物细胞系中的污染的准确性 靶核酸序列。 更具体地，样品可以含有从细胞系分离的细胞或核酸。 本发明还涉及使用基于PCR的测定法的检测试剂盒。 本发明还涉及使用特异性产生的与细胞色素c氧化酶I（COI）和/或细胞色素b的不同序列的特定序列或群体互补的核酸来检测，鉴定和定量特定生物体，生物体组， 细胞中的真核细胞或病毒组。