公开(公告)号：US20190292579A1

公开(公告)日：2019-09-26

申请号：US16333665

申请日：2017-09-18

Applicant: Aaron Kurt Neumann ,  Matthew S Graus ,  Jerilyn A Timlin

Inventor： Aaron Kurt Neumann ,  Matthew S Graus ,  Jerilyn A Timlin

IPC: C12Q1/04 ,  G01N21/64

Abstract: Methods and apparatus for rapid, culture free pathogen detection. The methods utilize optical spectroscopy techniques to identify and/or characterize pathogens in a sample via the detection of unique properties and/or analytes that are specific to particular pathogens.

公开(公告)号：US08686363B1

公开(公告)日：2014-04-01

申请号：US13609057

申请日：2012-09-10

Applicant: Jerilyn A. Timlin ,  Jesse S. Aaron

Inventor： Jerilyn A. Timlin ,  Jesse S. Aaron

IPC: G01J5/02

CPC classification number: G01N21/6458 ,  G01J3/2823 ,  G02B21/0032 ,  G02B21/0076

Abstract: A hyperspectral stimulated emission depletion (“STED”) microscope system for high-resolution imaging of samples labeled with multiple fluorophores (e.g., two to ten fluorophores). The hyperspectral STED microscope includes a light source, optical systems configured for generating an excitation light beam and a depletion light beam, optical systems configured for focusing the excitation and depletion light beams on a sample, and systems for collecting and processing data generated by interaction of the excitation and depletion light beams with the sample. Hyperspectral STED data may be analyzed using multivariate curve resolution analysis techniques to deconvolute emission from the multiple fluorophores. The hyperspectral STED microscope described herein can be used for multi-color, subdiffraction imaging of samples (e.g., materials and biological materials) and for analyzing a tissue by Förster Resonance Energy Transfer (“FRET”).

Abstract translation: 用于用多个荧光团（例如2至10个荧光团）标记的样品的高分辨率成像的高光谱激发的发射耗尽（“STED”）显微镜系统。 超光谱STED显微镜包括光源，被配置为产生激发光束和耗尽光束的光学系统，被配置用于将激发和耗尽光束聚焦在样本上的光学系统，以及用于收集和处理由相互作用产生的数据的系统 激发和耗尽光束与样品。 可以使用多变量曲线分析技术分析高光谱STED数据，以从多个荧光团解卷积发射。 本文所述的超光谱STED显微镜可用于样品（例如材料和生物材料）的多色，亚衍射成像和Förster共振能量转移（“FRET”）分析组织。