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1.发明授权公开(公告)号：US08354266B2
公开(公告)日：2013-01-15
申请号：US12621805
申请日：2009-11-19
Applicant: Wen-Hsiung Li , Ming-Che Shih , Chieh-Chen Huang , Jui-Jen Chang , Cheng-Yu Ho
Inventor： Wen-Hsiung Li , Ming-Che Shih , Chieh-Chen Huang , Jui-Jen Chang , Cheng-Yu Ho
CPC classification number: C07K14/33 , C12N1/22 , C12N9/00 , C12N9/2437 , C12N15/63 , C12P19/02 , C12Y302/01004
Abstract: A polycistronic expression cassette encoding proteins necessary for constructing a multi-enzyme complex was developed. Also disclosed herein is a host cell containing this polycistronic expression cassette and uses thereof in degrading biomass.
Abstract translation: 开发了编码构建多酶复合物所必需的蛋白质的多顺反子表达盒。 本文还公开了含有该多顺反子表达盒的宿主细胞及其在降解生物质中的用途。
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公开(公告)号：US08703474B2
公开(公告)日：2014-04-22
申请号：US13210637
申请日：2011-08-16
Applicant: Jui-Jen Chang , Cheng-Yu Ho , Chieh-Chen Huang , Ming-Che Shih , Wen-Hsiung Li
Inventor： Jui-Jen Chang , Cheng-Yu Ho , Chieh-Chen Huang , Ming-Che Shih , Wen-Hsiung Li
CPC classification number: C12R1/645 , C12P7/22 , C12P7/62 , C12P7/6436
Abstract: Disclosed are a novel flavor compound-producing yeast strain and methods of using the strain to produce flavor compounds.
Abstract translation: 公开了一种新颖的产生风味化合物的酵母菌株和使用该菌株产生风味化合物的方法。
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公开(公告)号：US20120040420A1
公开(公告)日：2012-02-16
申请号：US13210637
申请日：2011-08-16
Applicant: Jui-Jen Chang , Cheng-Yu Ho , Chieh-Chen Huang , Ming-Che Shih , Wen-Hsiung Li
Inventor： Jui-Jen Chang , Cheng-Yu Ho , Chieh-Chen Huang , Ming-Che Shih , Wen-Hsiung Li
CPC classification number: C12R1/645 , C12P7/22 , C12P7/62 , C12P7/6436
Abstract: Disclosed are a novel flavor compound-producing yeast strain and methods of using the strain to produce flavor compounds.
Abstract translation: 公开了一种新颖的产生风味化合物的酵母菌株和使用该菌株产生风味化合物的方法。
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公开(公告)号：US08003344B2
公开(公告)日：2011-08-23
申请号：US12068050
申请日：2008-02-01
Applicant: Chieh-Chen Huang , Jui-Jen Chang , Cheng-Yu Ho , Wei-En Chen , Jiunn-Jyi Lay , Chia-Hung Chou , Chang-Lung Han
Inventor： Chieh-Chen Huang , Jui-Jen Chang , Cheng-Yu Ho , Wei-En Chen , Jiunn-Jyi Lay , Chia-Hung Chou , Chang-Lung Han
Abstract: The present invention provides a microbial hydrogen-producing process, comprising: providing at least one Clostridium microbe and at least one Bacillus microbe; and co-culturing said at least one Clostridium microbe and said at least one Bacillus microbe in a fermentation culture system to produce hydrogen. The present invention also provides a microbial hydrogen-producing system, characterized by that the system comprises at least one Clostridium microbe and at least one Bacillus microbe, and it uses an organic waste medium as the substrate to perform a hydrogen-producing fermentation having high efficiency, high stability, and high reproducibility.
Abstract translation: 本发明提供一种微生物产氢方法，其包括：提供至少一种梭菌属微生物和至少一种芽孢杆菌属微生物; 并在发酵培养系统中共培养所述至少一种梭菌属微生物和所述至少一种芽孢杆菌属微生物以产生氢。 本发明还提供了一种微生物制氢系统，其特征在于，该系统包含至少一种梭菌属微生物和至少一种芽孢杆菌属微生物，并且使用有机废物介质作为底物进行具有高效率的产氢发酵 ，稳定性高，重现性高。
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5.发明申请公开(公告)号：US20110117630A1
公开(公告)日：2011-05-19
申请号：US12621805
申请日：2009-11-19
Applicant: Wen-Hsiung Li , Ming-Che Shih , Chieh-Chen Huang , Jui-Jen Chang , Cheng-Yu Ho
Inventor： Wen-Hsiung Li , Ming-Che Shih , Chieh-Chen Huang , Jui-Jen Chang , Cheng-Yu Ho
CPC classification number: C07K14/33 , C12N1/22 , C12N9/00 , C12N9/2437 , C12N15/63 , C12P19/02 , C12Y302/01004
Abstract: A polycistronic expression cassette encoding proteins necessary for constructing a multi-enzyme complex was developed. Also disclosed herein is a host cell containing this polycistronic expression cassette and uses thereof in degrading biomass.
Abstract translation: 开发了编码构建多酶复合物所必需的蛋白质的多顺反子表达盒。 本文还公开了含有该多顺反子表达盒的宿主细胞及其在降解生物质中的用途。
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Patent Agency Ranking
公开(公告)号：US09340794B2
公开(公告)日：2016-05-17
申请号：US13601146
申请日：2012-08-31
Applicant: Jui-Jen Chang , Feng-Ju Ho , Cheng-Yu Ho , Wen-Hsiung Li , Ming-Che Shih , Chieh-Chen Huang
Inventor： Jui-Jen Chang , Feng-Ju Ho , Cheng-Yu Ho , Wen-Hsiung Li , Ming-Che Shih , Chieh-Chen Huang
CPC classification number: C12N15/815
Abstract: Described is a method of introducing multiple nucleic acid molecules into the genome of a cell. The method includes providing a plurality of nucleic acid molecules, each of the plurality of nucleic acid molecules containing (a) a nucleic acid sequence operatively linked to a promoter sequence at the 5′ end of the nucleic acid molecule, and (b) an overlapping sequence at the 3′ end of the nucleic acid molecule.
Abstract translation: 描述了将多个核酸分子引入细胞的基因组中的方法。 所述方法包括提供多个核酸分子，所述多个核酸分子中的每一个含有（a）与所述核酸分子的5'末端的启动子序列可操作地连接的核酸序列，和（b）重叠 序列在核酸分子的3'末端。
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公开(公告)号：US20140248703A1
公开(公告)日：2014-09-04
申请号：US13601146
申请日：2012-08-31
Applicant: Jui-Jen Chang , Feng-Ju Ho , Cheng-Yu Ho , Wen-Hsiung Li , Ming-Che Shih , Chieh-Chen Huang
Inventor： Jui-Jen Chang , Feng-Ju Ho , Cheng-Yu Ho , Wen-Hsiung Li , Ming-Che Shih , Chieh-Chen Huang
IPC: C12N15/81
CPC classification number: C12N15/815
Abstract: Described is a method of introducing multiple nucleic acid molecules into the genome of a cell. The method includes providing a plurality of nucleic acid molecules, each of the plurality of nucleic acid molecules containing (a) a nucleic acid sequence operatively linked to a promoter sequence at the 5′ end of the nucleic acid molecule, and (b) an overlapping sequence at the 3′ end of the nucleic acid molecule.
Abstract translation: 描述了将多个核酸分子引入细胞基因组的方法。 所述方法包括提供多个核酸分子，所述多个核酸分子中的每一个含有（a）与所述核酸分子的5'末端的启动子序列可操作地连接的核酸序列，和（b）重叠 序列在核酸分子的3'末端。
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8.发明授权公开(公告)号：US08431371B2
公开(公告)日：2013-04-30
申请号：US13306056
申请日：2011-11-29
Applicant: Wen-Hsiung Li , Ming-Che Shih , Chieh-Chen Huang , Jui-Jen Chang , Cheng-Yu Ho
Inventor： Wen-Hsiung Li , Ming-Che Shih , Chieh-Chen Huang , Jui-Jen Chang , Cheng-Yu Ho
CPC classification number: C07K14/33 , C12N1/22 , C12N9/00 , C12N9/244 , C12N15/63 , C12N15/75 , C12P19/02 , C12P19/14 , C12Y302/01006 , Y02E50/16 , Y02E50/17
Abstract: An expression system for producing a multi-enzyme complex, the system including a nucleic acid molecule containing a promoter operatively linked to a nucleotide sequence including multiple genes encoding multiple enzymes that are components of the multi-enzyme complex.
Abstract translation: 一种用于产生多酶复合物的表达系统，该系统包含含有可操作地连接到核苷酸序列的启动子的核酸分子，该核苷酸序列包括编码作为多酶复合物的组分的多种酶的多个基因。
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9.发明申请公开(公告)号：US20120083012A1
公开(公告)日：2012-04-05
申请号：US13306056
申请日：2011-11-29
Applicant: Jui-Jen Chang , Cheng-Yu Ho , Ming-Che Shih , Chieh-Chen Huang , Wen-Hsiung Li
Inventor： Jui-Jen Chang , Cheng-Yu Ho , Ming-Che Shih , Chieh-Chen Huang , Wen-Hsiung Li
CPC classification number: C07K14/33 , C12N1/22 , C12N9/00 , C12N9/244 , C12N15/63 , C12N15/75 , C12P19/02 , C12P19/14 , C12Y302/01006 , Y02E50/16 , Y02E50/17
Abstract: An expression system for producing a multi-enzyme complex, the system including a nucleic acid molecule containing a promoter operatively linked to a nucleotide sequence including multiple genes encoding multiple enzymes that are components of the multi-enzyme complex.
Abstract translation: 一种用于产生多酶复合物的表达系统，该系统包含含有可操作地连接到核苷酸序列的启动子的核酸分子，该核苷酸序列包括编码作为多酶复合物的组分的多种酶的多个基因。
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公开(公告)号：US20090035812A1
公开(公告)日：2009-02-05
申请号：US12068050
申请日：2008-02-01
Applicant: Chieh-Chen Huang , Jui-Jen Chang , Cheng-Yu Ho , Wei-En Chen , Jiunn-Jyi Lay
Inventor： Chieh-Chen Huang , Jui-Jen Chang , Cheng-Yu Ho , Wei-En Chen , Jiunn-Jyi Lay
Abstract: The present invention provides a microbial hydrogen-producing process, comprising: providing at least one Clostridium microbe and at least one Bacillus microbe; and co-culturing said at least one Clostridium microbe and said at least one Bacillus microbe in a fermentation culture system to produce hydrogen. The present invention also provides a microbial hydrogen-producing system, characterized by that the system comprises at least one Clostridium microbe and at least one Bacillus microbe, and it uses an organic waste medium as the substrate to perform a hydrogen-producing fermentation having high efficiency, high stability, and high reproducibility.
Abstract translation: 本发明提供一种微生物产氢方法，其包括：提供至少一种梭菌属微生物和至少一种芽孢杆菌属微生物; 并在发酵培养系统中共培养所述至少一种梭菌属微生物和所述至少一种芽孢杆菌属微生物以产生氢。 本发明还提供了一种微生物制氢系统，其特征在于，该系统包含至少一种梭菌属微生物和至少一种芽孢杆菌属微生物，并且使用有机废物介质作为底物进行具有高效率的产氢发酵 ，稳定性高，重现性高。
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