公开(公告)号：US09651487B2

公开(公告)日：2017-05-16

申请号：US12037332

申请日：2008-02-26

申请人： Lloyd M. Smith ,  Matthew R. Lockett ,  Michael R. Shortreed ,  Robert M. Corn ,  Stephen Weibel ,  Robert J. Hamers ,  Bin Sun

发明人： Lloyd M. Smith ,  Matthew R. Lockett ,  Michael R. Shortreed ,  Robert M. Corn ,  Stephen Weibel ,  Robert J. Hamers ,  Bin Sun

IPC分类号： G02B5/04 ,  G01N21/552

CPC分类号： G01N21/553 ,  G02B5/04

摘要： SPR-compatible substrates for high density microarray fabrication and analyses are provided. Novel carbon-on-metal thin film substrate architecture permits the integration of surface plasmon resonance detection with photolithographically fabricated biomolecule arrays for the analysis of biomolecular interactions. The utility of the technology is shown in the analysis of specific DNA-DNA, DNA-RNA and DNA-protein binding interactions. These new substrates may be used to determine the secondary structure of RNA molecules, to probe the sequence-specific binding kinetics and affinity of proteins and small molecules, and as substrates for small-molecule combinatorial chemistry platforms for drug discovery applications.

公开(公告)号：US20130133419A1

公开(公告)日：2013-05-30

申请号：US13594518

申请日：2012-08-24

申请人： George M. Whitesides ,  Matthew R. Lockett ,  Katherine A. Mirica ,  Charles R. Mace ,  Robert D. Blackledge

发明人： George M. Whitesides ,  Matthew R. Lockett ,  Katherine A. Mirica ,  Charles R. Mace ,  Robert D. Blackledge

IPC分类号： G01N9/00

CPC分类号： G01N9/00 ,  G01N9/10

摘要： A method for determining the density of contact trace objects with magnetic levitation is described. The density of samples of glitter and of gunpowder was determined, and the feasibility of magnetic levitation as a possible means of characterizing forensic-related evidence is discussed. The magnetic levitation device (composed of two permanent magnets with like poles facing) and the method described provides a means of accurately determining the density of trace objects that is inexpensive, rapid, verifiable, provides documentation, is independent of the specific apparatus or analyst, and provides numerical values (rather than a comparison between questioned and known samples) that may be entered into a searchable database.

摘要翻译： 描述了一种用磁悬浮确定接触痕迹物体的密度的方法。 确定了闪光和火药样品的密度，讨论了磁悬浮作为鉴定法证相关证据的可能手段的可行性。 磁悬浮装置（由具有相同磁极的两个永磁体组成）和所述方法提供了一种准确地确定跟踪物体的密度的方法，该物体廉价，快速，可验证，提供文件，独立于具体装置或分析者， 并提供可输入到可搜索数据库中的数值（而不是有疑问和已知样本之间的比较）。

公开(公告)号：US20090141376A1

公开(公告)日：2009-06-04

申请号：US12037332

申请日：2008-02-26

申请人： Lloyd M. Smith ,  Matthew R. Lockett ,  Michael R. Shortreed ,  Robert M. Corn ,  Stephen Weibel ,  Robert J. Hamers ,  Bin Sun

发明人： Lloyd M. Smith ,  Matthew R. Lockett ,  Michael R. Shortreed ,  Robert M. Corn ,  Stephen Weibel ,  Robert J. Hamers ,  Bin Sun

IPC分类号： G02B5/04 ,  G01N21/55 ,  H01J49/00 ,  B32B38/00 ,  B05D5/06 ,  C23C14/34

CPC分类号： G01N21/553 ,  G02B5/04

摘要： SPR-compatible substrates for high density microarray fabrication and analyses are provided. Novel carbon-on-metal thin film substrate architecture permits the integration of surface plasmon resonance detection with photolithographically fabricated biomolecule arrays for the analysis of biomolecular interactions. The utility of the technology is shown in the analysis of specific DNA-DNA, DNA-RNA and DNA-protein binding interactions. These new substrates may be used to determine the secondary structure of RNA molecules, to probe the sequence-specific binding kinetics and affinity of proteins and small molecules, and as substrates for small-molecule combinatorial chemistry platforms for drug discovery applications.

摘要翻译： 提供了用于高密度微阵列制造和分析的SPR兼容基板。 新的碳 - 金属薄膜基板架构允许将表面等离子体共振检测与光刻制造的生物分子阵列整合以用于生物分子相互作用的分析。 该技术的实用性在特异性DNA-DNA，DNA-RNA和DNA-蛋白结合相互作用的分析中显示。 这些新的底物可用于确定RNA分子的二级结构，以探测序列特异性结合动力学和蛋白质和小分子的亲和力，以及作为用于药物发现应用的小分子组合化学平台的底物。