公开(公告)号：US20150273034A1

公开(公告)日：2015-10-01

申请号：US14675838

申请日：2015-04-01

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Alice L. Yu ,  John Yu

IPC: A61K39/00

CPC classification number: A61K39/0011 ,  A61K2039/55511 ,  A61K2039/6037 ,  A61K2039/6081 ,  A61K2039/627 ,  C12N15/1137 ,  C12N2310/14

Abstract: An immunogenic composition containing a glycan conjugate including a carrier protein, and a glycan including Globo H, an immunogenic fragment thereof, or stage-specific embryonic antigen-4 (SSEA-4), wherein the glycan is conjugated with the carrier protein through a linker.

Abstract translation: 含有载体蛋白的聚糖缀合物和包含Globo H，其免疫原性片段或阶段特异性胚胎抗原-4（SSEA-4）的聚糖的免疫原性组合物，其中所述聚糖通过接头与载体蛋白缀合 。

公开(公告)号：US20140363372A1

公开(公告)日：2014-12-11

申请号：US14223800

申请日：2014-03-24

Applicant: DEVELOPMENT CENTER FOR BIOTECHNOLOGY ,  CHANG GUNG MEMORIAL HOSPITAL ,  ACADEMIA SINICA

Inventor： Alice L. Yu ,  John Yu ,  Ya-Hui Wang ,  Chuan-Lung Hsu ,  Yi-Chang Chen ,  Ying-Yung Lok

IPC: C07K16/30 ,  G01N33/574 ,  C12Q1/68 ,  A61K51/10

CPC classification number: C07K16/3015 ,  A61K39/39558 ,  C07K16/30 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N33/57496

Abstract: A method for treating cancer includes administering to a subject in need thereof an antibody against a transmembrane and coiled-coil domains protein 3 (TMCC3), wherein the antibody binds to an epitope in an extracellular domain of TMCC3. The antibody binds to an epitope in an intercoil domain of TMCC3. A method of diagnosing or assessing a cancer condition includes assessing a level of expression or activity of a transmembrane and coiled-coil domains protein 3 (TMCC3) in a sample, wherein an increase in the level of expression of activity of TMCC3 as compared to a standard indicates the presence of cancer stem cells in the sample.

Abstract translation: 治疗癌症的方法包括向有需要的受试者施用针对跨膜和卷曲螺旋结构域蛋白3（TMCC3）的抗体，其中所述抗体结合TMCC3的细胞外结构域中的表位。 抗体结合TMCC3的线粒体结构域中的表位。 一种诊断或评估癌症病症的方法包括评估样品中跨膜和卷曲螺旋结构域蛋白3（TMCC3）的表达或活性水平，其中TMCC3的活性表达水平与 标准表明样品中存在癌干细胞。

公开(公告)号：US10533034B2

公开(公告)日：2020-01-14

申请号：US15866378

申请日：2018-01-09

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey Wong ,  Alice L. Yu ,  Kun-Hsien Lin ,  Tai-Na Wu

IPC: A61K39/39 ,  C07H15/18 ,  C07H15/04 ,  A61K31/7032 ,  C07H17/02 ,  A61K39/00

Abstract: Glycosphingolipids (GSLs) compositions and methods for iNKT-independent induction of chemokines are disclosed.

公开(公告)号：US09447193B2

公开(公告)日：2016-09-20

申请号：US14223800

申请日：2014-03-24

Applicant: Development Center for Biotechnology ,  Academia Sinica ,  Chang Gung Memorial Hospital

Inventor： Alice L. Yu ,  John Yu ,  Ya-Hui Wang ,  Chuan-Lung Hsu ,  Yi-Chang Chen ,  Ying-Yung Lok

IPC: A61K39/395 ,  C07K16/30 ,  G01N33/574 ,  C12Q1/68

CPC classification number: C07K16/3015 ,  A61K39/39558 ,  C07K16/30 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N33/57496

Abstract: A method for treating cancer includes administering to a subject in need thereof an antibody against a transmembrane and coiled-coil domains protein 3 (TMCC3), wherein the antibody binds to an epitope in an extracellular domain of TMCC3. The antibody binds to an epitope in an intercoil domain of TMCC3. A method of diagnosing or assessing a cancer condition includes assessing a level of expression or activity of a transmembrane and coiled-coil domains protein 3 (TMCC3) in a sample, wherein an increase in the level of expression of activity of TMCC3 as compared to a standard indicates the presence of cancer stem cells in the sample.

Abstract translation: 治疗癌症的方法包括向有需要的受试者施用针对跨膜和卷曲螺旋结构域蛋白3（TMCC3）的抗体，其中所述抗体结合TMCC3的细胞外结构域中的表位。 抗体结合TMCC3的线粒体结构域中的表位。 一种诊断或评估癌症病症的方法包括评估样品中跨膜和卷曲螺旋结构域蛋白3（TMCC3）的表达或活性水平，其中TMCC3的活性表达水平与 标准表明样品中存在癌干细胞。

公开(公告)号：US10918714B2

公开(公告)日：2021-02-16

申请号：US16175504

申请日：2018-10-30

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Alice L. Yu ,  Kun-Hsien Lin ,  Tai-Na Wu

IPC: A61K39/39 ,  C07H15/18 ,  A61K39/00

Abstract: Glycosphingolipids (GSLs) bearing α-glucose (α-Glc) that preferentially stimulate human invariant NKT (iNKT) cells are provided. GSLs with α-glucose (α-Glc) that exhibit stronger induction in humans (but weaker in mice) of cytokines and chemokines and expansion and/or activation of immune cells than those with α-galactose (α-Gal) are disclosed. GSLs bearing α-glucose (α-Glc) and derivatives of α-Glc with F at the 4 and/or 6 positions are provided. Methods for iNKT-independent induction of chemokines by the GSL with α-Glc and derivatives thereof are disclosed. Methods for immune stimulation in humans using GSLs with α-Glc and derivatives thereof are provided.

公开(公告)号：US09879042B2

公开(公告)日：2018-01-30

申请号：US14847835

申请日：2015-09-08

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Alice L. Yu ,  Kun-Hsien Lin ,  Tai-Na Wu

IPC: C07H17/02 ,  C07H15/18 ,  A61K39/39 ,  C07H15/04 ,  A61K39/00

CPC classification number: C07H15/18 ,  A61K31/7032 ,  A61K39/39 ,  A61K2039/55511 ,  A61K2039/585 ,  C07H15/04 ,  C07H17/02

Abstract: Glycosphingolipids (GSLs) compositions and methods for iNKT-independent induction of chemokines are disclosed.

公开(公告)号：US09603913B2

公开(公告)日：2017-03-28

申请号：US14675838

申请日：2015-04-01

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Alice L. Yu ,  John Yu

IPC: A61K39/00 ,  C12N15/113

CPC classification number: A61K39/0011 ,  A61K2039/55511 ,  A61K2039/6037 ,  A61K2039/6081 ,  A61K2039/627 ,  C12N15/1137 ,  C12N2310/14

Abstract: An immunogenic composition containing a glycan conjugate including a carrier protein, and a glycan including Globo H, an immunogenic fragment thereof, or stage-specific embryonic antigen-4 (SSEA-4), wherein the glycan is conjugated with the carrier protein through a linker.

公开(公告)号：US20160321394A1

公开(公告)日：2016-11-03

申请号：US15213479

申请日：2016-07-19

Applicant: Academia Sinica

Inventor： John Yu ,  Alice L. Yu ,  H.C. Wu ,  I-Ju Chen ,  Sheng-Hung Wang

IPC: G06F19/16 ,  G01N33/68 ,  G06F19/24 ,  C40B30/02

CPC classification number: G16B15/00 ,  A61K9/1271 ,  A61K47/62 ,  A61K47/6911 ,  A61K51/1234 ,  C07K5/1024 ,  C07K7/08 ,  G01N33/57492 ,  G16B35/00 ,  G16C20/60

Abstract: Disclosed herein are structure-based methods for ligand optimization. The methods involve the selection of a candidate ligand from a structural library based on the binding energy of the ligand with a cancer cell-surface protein. The binding energy is estimated from parameters including polar and mon-polar interactions between the ligand and the surface protein. In this way, candidate ligand(s) with desirable binding affinity to the cancer cell-surface protein can be selected.

Abstract translation: 本文公开了配体优化的基于结构的方法。 该方法包括基于配体与癌细胞表面蛋白的结合能从结构文库中选择候选配体。 从包括配体和表面蛋白质之间的极性和单极性相互作用的参数估计结合能。 以这种方式，可以选择对癌细胞表面蛋白具有所需结合亲和力的候选配体。

公开(公告)号：US20150071960A1

公开(公告)日：2015-03-12

申请号：US14480521

申请日：2014-09-08

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Alice L. Yu ,  Kun-Hsien Lin ,  Tai-Na Chen

IPC: A61K39/39 ,  C07H15/18

CPC classification number: A61K39/39 ,  A61K2039/55511 ,  A61K2039/57 ,  C07H15/18

Abstract: Glycosphingolipids (GSLs) bearing α-glucose (α-Glc) that preferentially stimulate human invariant NKT (iNKT) cells are provided. GSLs with α-glucose (α-Glc) that exhibit stronger induction in humans (but weaker in mice) of cytokines and chemokines and expansion and/or activation of immune cells than those with α-galactose (α-Gal) are disclosed. GSLs bearing α-glucose (α-Glc) and derivatives of α-Glc with F at the 4 and/or 6 positions are provided. Methods for iNKT-independent induction of chemokines by the GSL with α-Glc and derivatives thereof are disclosed. Methods for immune stimulation in humans using GSLs with α-Glc and derivatives thereof are provided.

Abstract translation: 提供了优先刺激人类不变NKT（iNKT）细胞的含有α-葡萄糖（α-Glc）的糖鞘糖脂（GSLs）。 公开了具有α-葡萄糖（α-Glc）的GSL，其具有比具有α-半乳糖（α-Gal）的细胞因子和趋化因子更强的诱导（但是在小鼠中较弱）和免疫细胞的膨胀和/或活化。 提供了带有α-葡萄糖（α-Glc）的GSL和α-Glc的衍生物，其中F在4和/或6位。 公开了通过具有α-Glc的GSL和其衍生物对iNKT独立诱导趋化因子的方法。 提供了使用具有α-Glc的GSL及其衍生物在人体中免疫刺激的方法。

公开(公告)号：US10111951B2

公开(公告)日：2018-10-30

申请号：US15689165

申请日：2017-08-29

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Alice L. Yu ,  Kun-Hsien Lin ,  Tai-Na Wu

IPC: A61K39/39 ,  C07H15/18 ,  A61K39/00

Abstract: Glycosphingolipids (GSLs) bearing α-glucose (α-Glc) that preferentially stimulate human invariant NKT (iNKT) cells are provided. GSLs with α-glucose (α-Glc) that exhibit stronger induction in humans (but weaker in mice) of cytokines and chemokines and expansion and/or activation of immune cells than those with α-galactose (α-Gal) are disclosed. GSLs bearing α-glucose (α-Glc) and derivatives of α-Glc with F at the 4 and/or 6 positions are provided. Methods for iNKT-independent induction of chemokines by the GSL with α-Glc and derivatives thereof are disclosed. Methods for immune stimulation in humans using GSLs with α-Glc and derivatives thereof are provided.