公开(公告)号：US20140220042A1

公开(公告)日：2014-08-07

申请号：US14254453

申请日：2014-04-16

Applicant: Academia Sinica

Inventor： Tse Wen Chang ,  Jiun-Bo Chen ,  Pheidias C. Wu ,  Alfur F. Hung

IPC: C07K16/28

CPC classification number: C07K16/2803 ,  A61K39/39566 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/30 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/732 ,  Y10S424/805 ,  Y10S424/81 ,  Y10S530/862 ,  Y10S530/868

Abstract: The invention pertains to the generation and utility of antibodies that can bind effectively to CεmX domain on membrane-bound IgE (mIgE) expressed on the surface of human B lymphocytes. The CεmX domain of 52 amino acid residues, located between the CH4 domain and the C-terminal membrane-anchor peptide on human membrane-bound epsilon chain, had been suggested as an antigenic site for immunological targeting of B cells expressing mIgE. Previous reported monoclonal antibodies, including a20, which bind to RADWPGPP (SEQ ID NO:1) peptide at the C-terminal of CεmX, have now been found to bind poorly to mIgE on human B cells. We have discovered that only monoclonal antibodies specific for certain segments, such as GLAGGSAQSQRAPDRVL (SEQ ID NO:2) and HSGQQQGLPRAAGGSVPHPR (SEQ ID NO:3), of CεmX can bind effectively to mIgE on human B cells and hence have the utility for targeting those B cells for the treatment of diseases mediated by IgE.

Abstract translation: 本发明涉及能够在人B淋巴细胞表面表达的膜结合IgE（mIgE）上有效结合C＆amp; mX结构域的抗体的产生和应用。 已经提出位于人膜结合的ε链上的CH4结构域和C末端膜锚定肽之间的52个氨基酸残基的Cys区域作为用于免疫靶向表达mIgE的B细胞的抗原位点。 目前已经发现，以前报道的，在C细胞C末端结合RADWPGPP（SEQ ID NO：1）肽的单克隆抗体，包括a20，现在已被发现与人B细胞上的mIgE结合不良。 我们已经发现，对于某些片段（例如GLAGGSAQSQRAPDRVL（SEQ ID NO：2）和HSGQQQGLPRAAGGSVPHPR（SEQ ID NO：3））特异性的单克隆抗体，C＆amp; mX可以有效地结合到人B细胞上的mIgE，因此具有效用 用于靶向这些B细胞用于治疗由IgE介导的疾病。

公开(公告)号：US09587034B2

公开(公告)日：2017-03-07

申请号：US14395572

申请日：2013-03-14

Applicant: Academia Sinica

Inventor： Tse Wen Chang ,  Jiun-Bo Chen ,  Chien-Jen Lin ,  Nien-Yi Chen

IPC: A61K39/395 ,  C07K16/42

CPC classification number: C07K16/4291 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/732

Abstract: Antibodies binding to junction regions between the CH4 and CεmX domains of membrane-bound IgE and uses thereof in treating IgE-mediated diseases such as allergic diseases.

Abstract translation: 结合到膜结合IgE的CH4和CεmX结构域之间的结合区域的抗体及其在治疗IgE介导的疾病如过敏性疾病中的用途。

公开(公告)号：US20160032017A1

公开(公告)日：2016-02-04

申请号：US14775204

申请日：2014-03-12

Applicant: ACADEMIA SINICA

Inventor： Tse Wen Chang ,  Chien-Jen Lin ,  Chien-Sheng Lu

IPC: C07K16/42

CPC classification number: C07K16/4291 ,  C07K7/04 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2317/73

Abstract: The present disclosure relates to anti-IgE antibodies that bind to novel antigenic epitopes of the CsmX domain, e.g., SVPHPRCHCGAGRA (SEQ ID NO: 4) and the uses thereof in treating IgE-mediated diseases.

Abstract translation: 本公开内容涉及结合CsmX结构域的新抗原表位的抗IgE抗体，例如SVPHPRCHCGAGRA（SEQ ID NO：4）及其在治疗IgE介导的疾病中的用途。

公开(公告)号：US08974794B2

公开(公告)日：2015-03-10

申请号：US14254453

申请日：2014-04-16

Applicant: Academia Sinica

Inventor： Tse Wen Chang ,  Jiun-Bo Chen ,  Pheidias C. Wu ,  Alfur F. Hung

IPC: A61K39/00 ,  C07K16/28 ,  C07K16/42 ,  A61K39/395

CPC classification number: C07K16/2803 ,  A61K39/39566 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/30 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/732 ,  Y10S424/805 ,  Y10S424/81 ,  Y10S530/862 ,  Y10S530/868

Abstract: The invention pertains to methods of using CεmX peptides (e.g., GLAGGSAQSQRAPDRVL; SEQ ID NO:2) that can bind effectively induce immune responses to membrane-bound IgE (mIgE) expressed on the surface of human B lymphocytes.

Abstract translation: 本发明涉及使用可以有效诱导在人B淋巴细胞表面上表达的膜结合IgE（mIgE）的免疫应答的C＆amp; MX肽（例如GLAGGSAQSQRAPDRVL; SEQ ID NO：2）的方法。

公开(公告)号：US09718891B2

公开(公告)日：2017-08-01

申请号：US14775204

申请日：2014-03-12

Applicant: Academia Sinica

Inventor： Tse Wen Chang ,  Chien-Jen Lin ,  Chien-Sheng Lu

IPC: A61K39/395 ,  C07K16/42 ,  C07K7/04

CPC classification number: C07K16/4291 ,  C07K7/04 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2317/73

Abstract: The present disclosure relates to anti-IgE antibodies that bind to novel antigenic epitopes of the CsmX domain, e.g., SVPHPRCHCGAGRA (SEQ ID NO: 4) and the uses thereof in treating IgE-mediated diseases.

公开(公告)号：US20150086558A1

公开(公告)日：2015-03-26

申请号：US14395572

申请日：2013-03-14

Applicant: Academia Sinica

Inventor： Tse Wen Chang ,  Jiun-Bo Chen ,  Chien-Jen Lin ,  Nien-Yi Chen

IPC: C07K16/42

CPC classification number: C07K16/4291 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/732

Abstract: Antibodies binding to junction regions between the CH4 and CεmX domains of membrane-bound IgE and uses thereof in treating IgE-mediated diseases such as allergic diseases.

Abstract translation: 抗体结合于膜结合IgE的CH4和Cγ区域之间的结合区域的抗体及其在治疗IgE介导的疾病如过敏性疾病中的用途。