公开(公告)号：US20220144920A1

公开(公告)日：2022-05-12

申请号：US17420231

申请日：2020-01-02

Applicant: AMGEN INC.

Inventor： Jennitte LeAnn STEVENS ,  Deniz TEMEL ,  Bram ESTES ,  Neeraj Jagdish AGRAWAL

IPC: C07K16/00

Abstract: Presented herein are methods directed to engineering monoclonal antibodies and antibody variants to improve stability and their production in culture. Specifically, the monoclonal antibodies can be engineered at heavy chain residue 56 (AHo numbering) to a glycine, alanine, or serine, and/or engineered at position 80 (AHo) to be a hydrophobic residue such as alanine, isoleucine, phenylalanine, leucine, methionine, or valine.

公开(公告)号：US20240182600A1

公开(公告)日：2024-06-06

申请号：US18556248

申请日：2022-04-19

Applicant: AMGEN INC.

Inventor： Danyang GONG ,  Bram ESTES ,  Zhulun WANG ,  Fernando GARCES

IPC: C07K16/46

CPC classification number: C07K16/468 ,  C07K2317/31 ,  C07K2317/526

Abstract: The clinical potential of multispecific antibodies like bispecific and trispecific antibodies shows great promise for targeting complex diseases. However, the generation of those molecules presents great challenges particularly in regard to achieving acceptable expression levels free from mis-paired polypeptides. The presently claimed invention is directed to multispecific antigen binding proteins which improve upon existing charge pair technologies by redistributing the engineered charges within the CH3 regions of a heteromultimer.

公开(公告)号：US20220389119A1

公开(公告)日：2022-12-08

申请号：US17775063

申请日：2020-11-06

Applicant: AMGEN INC.

Inventor： Bram ESTES ,  Fernando GARCES ,  Zhulun WANG ,  Mark DARIS

IPC: C07K16/46

Abstract: The present invention relates to heterodimers comprising antibody CH3 domains and mutations useful for the facilitation of the formation of heterodimers. Methods of optimizing purification of the heterodimers at certain pHs in also disclosed.

公开(公告)号：US20220281966A1

公开(公告)日：2022-09-08

申请号：US17630437

申请日：2020-07-24

Applicant: AMGEN INC.

Inventor： Agnieszka KIELCZEWSKA ,  Ian Nevin FOLTZ ,  Palaniswami RATHANASWAMI ,  Maria Sheena AMADOR ,  Bram ESTES ,  Igor D'ANGELO

IPC: C07K16/24 ,  A61P11/00 ,  A61P11/08 ,  A61P11/06 ,  A61P17/00

Abstract: The present invention is related to antibodies directed to IL-13 and uses of such antibodies. For example, in accordance with the present invention, there are provided human monoclonal antibodies directed to IL-13. Isolated polynucleotide sequences encoding, and amino acid sequences comprising, heavy and light chain immunoglobulin molecules, particularly sequences corresponding to contiguous heavy and light chain sequences spanning the framework regions (FR's) and/or complementarity determining regions (CDR's), are provided. Additionally, methods of using these antibodies to treat patients are also provided.