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公开(公告)号:US20200256843A1
公开(公告)日:2020-08-13
申请号:US16864672
申请日:2020-05-01
申请人: ABBOTT LABORATORIES
发明人: Jeffrey B. Huff , Mark A. Hayden , Peter J. Karabatsos , Andrew S. Schapals , Anthony S. Muerhoff , M. Felicia Bogdan , Thomas Leary , Shelley R. Holets-McCormack , Sophie Laurenson , Andrew T. Fischer , Richard Haack , Stefan Hershberger , Dustin House , Lei Qiao , M. Shawn Murphy , Mark R. Pope , Edna M. Prieto-Ballengee , QiaoQiao Ruan , Pathik Soni , Sergey Tetin , Lyle Yarnell
IPC分类号: G01N33/487 , G01N33/53 , B01L3/00 , G01N33/543
摘要: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.
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公开(公告)号:US20180188230A1
公开(公告)日:2018-07-05
申请号:US15724213
申请日:2017-10-03
申请人: ABBOTT LABORATORIES
发明人: Jeffrey B. Huff , Mark A. Hayden , Peter J. Karabatsos , Andrew S. Schapals , Anthony S. Muerhoff , Felicia Bogdan , Thomas Leary , Shelley R. Holets-McCormack , Sophie Laurenson , Andrew T. Fischer , Richard Haack , Stefan Hershberger , Dustin House , Lei Qiao , M. Shawn Murphy , Mark R. Pope , Edna M. Prieto-Ballengee , QiaoQiao Ruan , Pathik Soni , Sergey Tetin , Lyle Yarnell
IPC分类号: G01N33/487 , G01N33/543 , B01L3/00
CPC分类号: G01N33/48721 , B01L3/502761 , B01L2300/0645 , B01L2400/0406 , G01N33/5308 , G01N33/54306 , G01N2458/10
摘要: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.
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公开(公告)号:US11022598B2
公开(公告)日:2021-06-01
申请号:US15724213
申请日:2017-10-03
申请人: ABBOTT LABORATORIES
发明人: Jeffrey B. Huff , Mark A. Hayden , Peter J. Karabatsos , Andrew S. Schapals , Anthony S. Muerhoff , M. Felicia Bogdan , Thomas Leary , Shelley R. Holets-McCormack , Sophie Laurenson , Andrew T. Fischer , Richard Haack , Stefan Hershberger , Dustin House , Lei Qiao , M. Shawn Murphy , Mark R. Pope , Edna M. Prieto-Ballengee , QiaoQiao Ruan , Pathik Soni , Sergey Tetin , Lyle Yarnell
IPC分类号: G01N33/487 , G01N33/543 , G01N33/53 , B01L3/00
摘要: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.
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公开(公告)号:US20180095067A1
公开(公告)日:2018-04-05
申请号:US15724200
申请日:2017-10-03
申请人: Abbott Laboratories
发明人: Jeffrey B. Huff , Mark A. Hayden , Peter J. Karabatsos , Andrew S. Schapals , Anthony S. Muerhoff , Felicia Bogdan , Thomas Leary , Shelley R. Holets-McCormack , Sophie Laurenson , Andrew T. Fischer , Richard Haack , Stefan Hershberger , Dustin House , Lei QIAO , M. Shawn Murphy , Mark R. Pope , Edna M. Prieto-Ballengee , QiaoQiao Ruan , Pathik Soni , Sergey Tetin , Lyle Yarnell , John M. Robinson
IPC分类号: G01N33/487 , G01N33/53
CPC分类号: G01N33/48721 , G01N33/5302 , G01N33/54326 , G01N33/5438
摘要: Integrated microfluidic and analyte detection devices are disclosed, along with methods of detecting target analytes. Digital microfluidic and analyte detection devices include a first substrate and a second substrate aligned generally parallel to each other to define a gap therebetween, the first substrate including a plurality of electrodes to generate electrical actuation forces on a liquid droplet disposed in the gap; at least one reagent disposed on at least one of the first substrate or the second substrate and configured to be carried by the liquid droplet; and an analyte detection device in fluid communication with the gap, wherein the plurality of electrodes are configured to move the liquid droplet towards the analyte detection device.
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