公开(公告)号：US20030215791A1

公开(公告)日：2003-11-20

申请号：US10277916

申请日：2002-10-23

Applicant: Applied Spectral Imaging Ltd.

Inventor： Yuval Garini ,  Nir Katzir ,  Irit Bar-Am ,  Uri Milman ,  Eli Horn ,  Yacov Malinovich ,  Terry Hammill ,  Vladimir Cherepakhin

IPC: C12Q001/70 ,  C12Q001/68 ,  G01N033/53 ,  G01N033/554 ,  G01N033/569 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: B82Y5/00 ,  B82Y10/00 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/54373

Abstract: A method of detecting the presence, absence and/or level of a plurality of analytes-of-interest in a sample, the method comprisES: (a) providing a plurality of objects, each of the plurality of objects having a predetermined, measurable and different imagery characteristic, and further having a predetermined and specific affinity to one analyte of the plurality of analytes-of-interest, each the imagery characteristic corresponding to one the predetermined specific affinity, hence each the imagery characteristic corresponds to one analyte of the plurality of analytes-of interest; (b) providing at least one affinity moiety having a predetermined and specific affinity or predetermined and specific affinities to the plurality of analytes-of-interest, each the affinity moiety having a predetermined, measurable response to light; (c) combining the objects, the at least one affinity moiety and the sample under conditions for affinity binding; and (d) simultaneously determining, for each object of the plurality of objects an imagery characteristic, and for at least a portion of the at least one affinity moiety a response to light, thereby detecting the presence, absence and/or level of the plurality of analytes-of-interest in the sample.

Abstract translation: 一种检测样本中多个感兴趣分析物的存在，不存在和/或等级的方法，所述方法包括：（a）提供多个对象，所述多个对象中的每一个具有预定的，可测量的和 不同的图像特征，并且还对多个感兴趣分析物中的一个分析物具有预定和特异性的亲和力，每个图像特征对应于一个预定的特异亲和力，因此每个图像特征对应于多个 分析物 - 兴趣; （b）提供至少一个亲和部分，其具有对多个感兴趣分析物的预定和特异性亲和力或预定和特异性亲和力，每个亲和部分具有对光的预定的可测量响应; （c）在用于亲和结合的条件下将所述目标，所述至少一个亲和部分和所述样品组合; 以及（d）对于所述多个对象的每个对象同时确定图像特征，以及对所述至少一个亲和部分的至少一部分对光的响应，从而检测所述多个对象的存在，不存在和/或等级 的样品中感兴趣的分析物。