公开(公告)号：US20040259099A1

公开(公告)日：2004-12-23

申请号：US10496588

申请日：2004-05-24

Inventor： Takamasa Katoh ,  Takeo Morimoto

IPC: C12Q001/68 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50 ,  H04L009/32

CPC classification number: G16B20/00 ,  G06Q50/24 ,  G16B30/00

Abstract: The present invention provides a highly-safe information processing system that is capable of effectively using nucleotide sequence information differences between individual organisms to offer semantic information useful for each individual organism while properly preventing leakage and illegal use of nucleotide sequence information. Further, the present invention includes steps a and b. Step a is performed to acquire either encrypted nucleotide sequence-related information or cryptographic key that corresponds to positional information indicating a position within a nucleotide sequence. Step b is performed to acquire the encrypted nucleotide sequence-related information or cryptographic key, whichever is not acquired in said step a, decrypt, with the cryptographic key, the encrypted nucleotide sequence-related information corresponding to the positional information compliant at least with a request for an object and/or a service, and acquire the nucleotide sequence-related information corresponding to the positional information compliant at least with the request for an object and/or a service.

Abstract translation: 本发明提供了一种高度安全的信息处理系统，其能够有效地使用个体生物体之间的核苷酸序列信息差异来提供对每个个体生物有用的语义信息，同时适当地防止核苷酸序列信息的泄漏和非法使用。 此外，本发明包括步骤a和b。 执行步骤a以获取对应于指示核苷酸序列内的位置的位置信息的加密的核苷酸序列相关信息或加密密钥。 执行步骤b以获取加密的核苷酸序列相关信息或加密密钥（以所述步骤a中未获取的加密密钥）用密码密钥解密对应于位置信息的加密的核苷酸序列相关信息至少符合 请求对象和/或服务，并且至少根据对象和/或服务的请求获取与至少符合的位置信息相对应的核苷酸序列相关信息。

公开(公告)号：US20040254774A1

公开(公告)日：2004-12-16

申请号：US10802516

申请日：2004-03-17

Inventor： Stewart Loh ,  Mark C. Butler ,  Jeung-Hoi Ha ,  Tracy L. Radley

IPC: G06G007/48 ,  G01N033/48

CPC classification number: C12N9/22 ,  C07K2319/00

Abstract: The present invention is a model for a mutually exclusive domain folding molecular switch comprising a two-domain, bifunctional fusion protein wherein the free energy released by the folding of a first domain of the fusion protein drives an unfolding of a second domain of the fusion protein, and vice versa. The molecular structure of the fusion protein is engineered so that, at any time, the folding of the first domain necessarily unfolds the other domain, and vice versa, thereby making the folded and unfolded states of the first and second domains mutually exclusive. This is accomplished by the insertion of an exemplary insert protein into a surface loop of an exemplary target protein subject to a novel structural design criterion wherein the N-C terminal length of the exemplary insert protein is at least two-times greater than the Cnull-Cnull length of the surface loop of the exemplary target protein.

Abstract translation: 本发明是包含双结构域双功能融合蛋白的互斥域折叠分子开关的模型，其中通过折叠融合蛋白的第一结构域释放的自由能驱动融合蛋白的第二结构域的解折叠 ，反之亦然。 设计融合蛋白的分子结构，使得在任何时候，第一结构域的折叠必然展开另一个域，反之亦然，从而使第一和第二域的折叠和展开状态相互排斥。 这是通过将示例性插入蛋白插入到示例性靶蛋白的表面环中实现的，其具有新颖的结构设计标准，其中示例性插入蛋白的NC端长度比Calpha-Calpha长度的至少两倍 的示例性靶蛋白的表面环。

公开(公告)号：US20040254735A1

公开(公告)日：2004-12-16

申请号：US10462056

申请日：2003-06-13

Inventor： Hans Werner Horn ,  Andreas Kraemer ,  Julia Elizabeth Rice

IPC: G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G06F19/705

Abstract: The virtual screening of a database of molecules is based on explicit three-dimensional molecular superpositions. The torsional flexibility of the database molecules is taken fully into account, and an arbitrary number of conformation-dependent molecular features may be considered. A fragmentation-reassembly approach is utilized, which allows for an efficient sampling of the conformational space. A fast clique-based pattern-matching algorithm generates alignments of pairs of adjacent molecular fragments on the (rigid) query molecule that are subsequently reassembled to complete database molecules. Using conventional molecular features (hydrogen bond donors and acceptors, charges, and hydrophobic groups), it is possible to rapidly produce accurate alignments of medium-sized drug-like molecules. Examples with a test database containing a diverse set of 1780 drug-like molecules (including all conformers) show that average query processing times of the order of 0.1 seconds per molecule can be achieved on a PC, depending on the size of the query molecule.

Abstract translation: 分子数据库的虚拟筛选基于显式的三维分子叠加。 充分考虑数据库分子的扭转灵活性，并且可以考虑任意数量的构象依赖性分子特征。 利用碎片重组方法，可以对构象空间进行有效的采样。 基于快速基于组合的模式匹配算法产生对（刚性）查询分子上的相邻分子片段对的比对，随后重新组装以完成数据库分子。 使用常规分子特征（氢键供体和受体，电荷和疏水基团），可以快速产生中等大小的药物样分子的精确对准。 含有多种1780种药物样分子（包括所有构象异构体）的测试数据库的示例显示，根据查询分子的大小，可以在PC上实现每分子0.1秒的平均查询处​​理时间。

公开(公告)号：US20040249576A1

公开(公告)日：2004-12-09

申请号：US10820466

申请日：2004-03-31

Applicant: Xencor

Inventor： John Rudolph Desjarlais ,  Jonathan Zalevsky

IPC: G06F019/00 ,  G01N033/48 ,  G01N033/50 ,  G01N031/00

CPC classification number: C07K1/1077 ,  A61K47/60 ,  C07K14/505 ,  C07K14/525 ,  C07K14/61 ,  C07K2299/00

Abstract: The present invention relates to the use of simulation technology to rationally optimize the locations and sizes of attached polymeric moieties for modification of therapeutic proteins and the proteins generated from this method.

Abstract translation: 本发明涉及使用模拟技术来合理优化连接的聚合物部分的位置和大小，用于修饰治疗性蛋白质和由该方法产生的蛋白质。

公开(公告)号：US20040248237A1

公开(公告)日：2004-12-09

申请号：US10486491

申请日：2004-02-09

Inventor： Michael G. Petit

IPC: G01N033/48 ,  G01N001/30

CPC classification number: G01N1/36 ,  G01N1/06 ,  G01N1/30 ,  Y10T436/2525

Abstract: An apparatus and method for preserving cytological specimens for histological or histopathological use. A cytological sample from a biological tissue such as a tumor or a tumor cell line is dehydrated, disbursed and evenly distributed throughout a volume of molten material. The molten material is then drawn into a tubular member such as a pipette and allowed to solidify. Upon solidification, the cylindrical specimen is partially or completely extrudedfrom the tube for further processing, including fixation, dehydration and molten paraffin infiltration and embedding as required for presentation to a sectioning device such as a microtome. Thin circular slices of the cylindrical specimen are removed from the cylindrical specimen and transferred to a slide, fixed and stained as desired. The device and method enable the production of a slide bearing a spatially homogeneous distribution of cytological material for microscopic examination.

Abstract translation: 一种用于保留细胞学标本用于组织学或组织病理学用途的装置和方法。 来自生物组织如肿瘤细胞系的细胞学样品被脱水，分散并均匀分布在一定体积的熔融材料中。 然后将熔融的材料拉入诸如移液管的管状构件中并使其固化。 固化后，将圆筒形样品从管中部分或完全挤出，用于进一步加工，包括固定，脱水和熔融石蜡渗透，并根据需要将其包埋到切片装置如切片机上。 将圆柱形试样的圆形圆形切片从圆柱形样品中取出并转移到载玻片上，固定并根据需要进行染色。 该装置和方法使得能够生产具有用于显微镜检查的细胞学材料的空间均匀分布的载玻片。

公开(公告)号：US20040242454A1

公开(公告)日：2004-12-02

申请号：US10452705

申请日：2003-06-02

Inventor： Stephen I. Gallant

IPC: A61K031/00 ,  G06F017/60 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G06Q50/24 ,  G16C20/50 ,  G16C20/70

Abstract: A disclosed method of developing a drug comprises, in one embodiment: selecting a sub-group of constituent drugs from a potential group, wherein the selection is made on the basis that an expected effectiveness of a combination drug comprising all of the compounds of the selected sub-group exceeds a pre-determined minimum threshold measure of expected effectiveness against a target of the drug, and such that a side-effect measure for each expected side-effect of the combination drug is less than a corresponding pre-determined maximum threshold measure of side-effect tolerability for each side-effect; and selecting a dosage for each drug of the sub-group such that each dosage is less than a dosage that would be used as a fully effective dosage of the drug when used individually against the target of the drug. Related methods of treating patients, combination drug formulations, and computer-implementations of the methods are also disclosed.

Abstract translation: 所公开的开发药物的方法包括在一个实施方案中：从潜在的组中选择成分药物的亚组，其中所述选择基于包含所选择的所有化合物的组合药物的预期有效性 子组超过对药物靶标的预期有效性的预定最小阈值度量，并且使得组合药物的每种预期副作用的副作用测量值小于相应的预定最大阈值量度 的副作用耐受性; 并且选择每个药物的剂量，使得每个剂量小于当单独用于药物靶标时用作药物的完全有效剂量的剂量。 还公开了治疗患者的相关方法，组合药物制剂和方法的计算机实施。

公开(公告)号：US20040241752A1

公开(公告)日：2004-12-02

申请号：US10877122

申请日：2004-06-25

Inventor： Emory V. Anderson ,  Jerome Lapointe ,  Ricardo Martinez ,  Gail Marzolf ,  Ronald Pong ,  Lynn Jones ,  Robert O. Hussa ,  Edward Nemec ,  Andrew E. Senyei ,  Duane DeSieno

IPC: G01N033/53 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G01N21/474 ,  G01N21/8483 ,  G01N33/558 ,  G01N33/6887 ,  G01N33/689 ,  G01N2333/78 ,  G01N2800/368 ,  G06F19/00 ,  G06F19/321 ,  G16H10/40 ,  G16H10/60 ,  G16H15/00 ,  G16H50/20 ,  Y02A90/26 ,  Y10S436/811 ,  Y10S436/814

Abstract: Systems and methods for medical diagnosis or risk assessment for a patient are provided. These systems and methods are designed to be employed at the point of care, such as in emergency rooms and operating rooms, or in any situation in which a rapid and accurate result is desired. The systems and methods process patient data, particularly data from point of care diagnostic tests or assays, including immunoassays, electrocardiograms, X-rays and other such tests, and provide an indication of a medical condition or risk or absence thereof. The systems include an instrument for reading or evaluating the test data and software for converting the data into diagnostic or risk assessment information.

Abstract translation: 提供了用于医疗诊断或患者风险评估的系统和方法。 这些系统和方法被设计为在护理点使用，例如在急诊室和手术室中，或在需要快速和准确的结果的任何情况下使用。 系统和方法处理患者数据，特别是来自护理诊断测试或测定的数据，包括免疫测定，心电图，X射线和其他此类测试，并提供医疗状况或其风险或不存在的指示。 系统包括用于读取或评估测试数据的工具，以及用于将数据转换为诊断或风险评估信息的软件。

公开(公告)号：US20040241741A1

公开(公告)日：2004-12-02

申请号：US10446865

申请日：2003-05-29

Inventor： Rodney Levine ,  Mary Ann Robinson

IPC: G01N033/53 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G01N33/6803 ,  G01N33/6812 ,  G16B30/00

Abstract: An unidentified purified protein can be uniquely identified by combining one or more physical parameters of the protein and an accurate amino acid content. The protein can be identified by input of the one or more physical parameters and the amino acid content into an interface that outputs data from a protein database.

Abstract translation: 可以通过组合蛋白质的一个或多个物理参数和精确的氨基酸含量来唯一地鉴定未鉴定的纯化蛋白质。 可以通过将一个或多个物理参数和氨基酸含量输入到从蛋白质数据库输出数据的界面来鉴定蛋白质。

公开(公告)号：US20040241738A1

公开(公告)日：2004-12-02

申请号：US10884299

申请日：2004-07-02

Inventor： David H. Stewart ,  John W. Groelke ,  H. Holden Thorp ,  Allen E. Eckhardt

IPC: C12Q001/68 ,  G01N033/53 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50 ,  G06F013/42

CPC classification number: B82Y30/00 ,  B82Y5/00 ,  C12Q1/6825 ,  G01N33/5438 ,  C12Q2565/607 ,  C12Q2563/137 ,  C12Q2563/113

Abstract: A method of detecting binding interactions and target molecules, such as proteins, protein fragments, recombinant proteins, recombinant protein fragments, extracellular matrix proteins, ligands, carbohydrates, steroids, hormones, drugs, drug candidates, immunoglobulins and receptors of eukaryotic, prokaryotic or viral origin, by mediated electrochemistry using labels that react with transition metal mediator complexes in a detectable catalytic redox reaction. These labels are attached directly to binders, target molecules, surrogate target molecules, or to affinity ligands capable of binding to the target or to surrogate target molecules capable of competing with the target for binding to another binder. The labels can be naturally present (endogenous) in the binder, target or affinity ligand, or constructed by the covalent attachment of the label to the binder, target, affinity ligand or surrogate target (exogenous).

Abstract translation: 检测结合相互作用和靶分子的方法，例如蛋白质，蛋白质片段，重组蛋白，重组蛋白片段，细胞外基质蛋白，配体，碳水化合物，类固醇，激素，药物，候选药物，免疫球蛋白和真核，原核或病毒的受体 通过介导的电化学使用在可检测的催化氧化还原反应中与过渡金属介体络合物反应的标记。 这些标记物直接连接到粘合剂，靶分子，替代靶分子，或与能够结合靶的亲和配体或替代能与靶标竞争结合另一种粘合剂的靶分子。 标签可以在粘合剂，靶或亲和配体中天然存在（内源的），或者通过标记共价连接到粘合剂，靶，亲和配体或替代靶（外源的）上构建。

公开(公告)号：US20040241661A1

公开(公告)日：2004-12-02

申请号：US10449136

申请日：2003-05-29

Inventor： Arindam Bhattacharjee

IPC: C12Q001/68 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: C40B30/04 ,  C12Q1/6816 ,  C12Q1/6837 ,  G01N33/58 ,  G16B25/00 ,  C12Q2565/102

Abstract: Methods of determining the amount of an analyte in a mixture of analytes are provided. The methods involve contacting a sample of analytes that is labeled with two or more distinguishably detectable labels with a probe for the analyte, and determining the amounts of the two or more distinguishably detectable labels bound with the probe. In certain embodiments, the methods include averaging the amounts of the two or more labels in order to determine the amount of analyte in the sample. Kits are provided for performing the invention. The subject invention finds use in a variety of different applications, including gene expression analysis, DNA sequencing, mutation detection and other genomics and proteomics applications.

Abstract translation: 提供了测定分析物混合物中分析物量的方法。 所述方法包括将标记有两个或多个可区分可检测标记的分析物样品与分析物的探针接触，并测定与探针结合的两个或更多个可区分地可检测的标记物的量。 在某些实施方案中，所述方法包括平均两个或更多个标记的量，以便确定样品中分析物的量。 提供用于执行本发明的套件。 本发明可用于各种不同的应用，包括基因表达分析，DNA测序，突变检测和其他基因组学和蛋白质组学应用。