公开(公告)号：US20230287486A1

公开(公告)日：2023-09-14

申请号：US18055398

申请日：2022-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. REGAN ,  Serge SAXONOV ,  Michael Y. LUCERO ,  Benjamin J. HINDSON ,  Phillip BELGRADER ,  Simant DUBE ,  Austin SO ,  Jeffrey Clark MELLEN ,  Nicholas Jack HEREDIA ,  Kevin D. NESS ,  Billy W. COLSTON, Jr.

IPC: C12Q1/6858 ,  C12Q1/683 ,  C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6858 ,  C12Q1/683 ,  C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/172 ,  C12Q2535/125

Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.