公开(公告)号：US20140302503A1

公开(公告)日：2014-10-09

申请号：US14201752

申请日：2014-03-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam Lowe ,  Geoff McDermott ,  Chris Hindson ,  Erin Chia ,  Amy Hiddessen ,  Ben Hindson ,  Chunxiao Han ,  Yaqi Wang

IPC: C12Q1/68 ,  B01L7/00

CPC classification number: C12Q1/6804 ,  B01L3/502784 ,  B01L7/52 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q2527/125 ,  C12Q2563/159 ,  C12Q2537/143

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

Abstract translation: 本公开提供了用于在聚合酶链反应测定中检测核酸的方法，装置，系统和组合物，例如液滴数字聚合酶链反应（ddPCR）测定。 本公开提供了使用插层染料在ddPCR测定中检测核酸的方法，装置，系统和组合物。 具有至少一种含氟表面活性剂和至少一种非离子非氟表面活性剂的双表面活性剂体系可用于液滴产生和核酸检测。