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公开(公告)号：US10150786B2
公开(公告)日：2018-12-11
申请号：US15697313
申请日：2017-09-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Adam Lowe , Chunxiao Han
Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.
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公开(公告)号：US20170362258A1
公开(公告)日：2017-12-21
申请号：US15697313
申请日：2017-09-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Adam Lowe , Chunxiao Han
CPC classification number: C07F7/1804 , C08G77/46 , C08G77/50 , C08L83/12 , C08L83/14 , G01N33/5436
Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.
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3.发明申请公开(公告)号：US20140302503A1
公开(公告)日：2014-10-09
申请号：US14201752
申请日：2014-03-07
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Adam Lowe , Geoff McDermott , Chris Hindson , Erin Chia , Amy Hiddessen , Ben Hindson , Chunxiao Han , Yaqi Wang
CPC classification number: C12Q1/6804 , B01L3/502784 , B01L7/52 , C12Q1/6844 , C12Q1/6851 , C12Q2527/125 , C12Q2563/159 , C12Q2537/143
Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
Abstract translation: 本公开提供了用于在聚合酶链反应测定中检测核酸的方法，装置，系统和组合物，例如液滴数字聚合酶链反应（ddPCR）测定。 本公开提供了使用插层染料在ddPCR测定中检测核酸的方法，装置，系统和组合物。 具有至少一种含氟表面活性剂和至少一种非离子非氟表面活性剂的双表面活性剂体系可用于液滴产生和核酸检测。
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Patent Agency Ranking
公开(公告)号：US20180135100A1
公开(公告)日：2018-05-17
申请号：US15818601
申请日：2017-11-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Adam Lowe , Geoff McDermott , Christopher M. Hindson , Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Chunxiao Han , Yaqi Wang
IPC: C12Q1/6804 , C12Q1/6844 , C12Q1/6851
Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
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公开(公告)号：US10676778B2
公开(公告)日：2020-06-09
申请号：US15818601
申请日：2017-11-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Adam Lowe , Geoff McDermott , Christopher M. Hindson , Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Chunxiao Han , Yaqi Wang
IPC: C12Q1/68 , C12Q1/6804 , B01L7/00 , C12Q1/6844 , C12Q1/6851 , B01L3/00
Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
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公开(公告)号：US09822393B2
公开(公告)日：2017-11-21
申请号：US14201752
申请日：2014-03-07
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Adam Lowe , Geoff McDermott , Christopher M. Hindson , Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Chunxiao Han , Yaqi Wang
CPC classification number: C12Q1/6804 , B01L3/502784 , B01L7/52 , C12Q1/6844 , C12Q1/6851 , C12Q2527/125 , C12Q2563/159 , C12Q2537/143
Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
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公开(公告)号：US09758535B2
公开(公告)日：2017-09-12
申请号：US14216006
申请日：2014-03-17
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Adam Lowe , Chunxiao Han
CPC classification number: C07F7/1804 , C08G77/46 , C08G77/50 , C08L83/12 , C08L83/14 , G01N33/5436
Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.
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公开(公告)号：US20140272995A1
公开(公告)日：2014-09-18
申请号：US14216006
申请日：2014-03-17
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Adam Lowe , Chunxiao Han
CPC classification number: C07F7/1804 , C08G77/46 , C08G77/50 , C08L83/12 , C08L83/14 , G01N33/5436
Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.
Abstract translation: 用于制备和使用包括硅油和硅氧烷表面活性剂的乳液的方法和组合物的系统。 乳液可以包括设置在包括硅油和硅氧烷表面活性剂的连续相中的水性液滴。 水滴可以含有任选地部分占据的分析物和/或发光（例如光致发光）报告物。 分析物的测定可以用液滴进行。 在一些情况下，可以从液滴中检测信号，并且可以基于该信号来确定分析物的特征，例如分析物水平或活性。
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