公开(公告)号：US12128415B2

公开(公告)日：2024-10-29

申请号：US17195206

申请日：2021-03-08

申请人： Pattern Bioscience, Inc.

发明人： Ross Johnson ,  Jonathan Isom ,  David Bussian ,  Nicolas Arab

IPC分类号： B01L9/00 ,  B01L1/02 ,  B01L3/00 ,  G01N21/31

CPC分类号： B01L9/527 ,  B01L1/02 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  G01N21/31 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0663 ,  B01L2400/049

摘要： An apparatus for loading and imaging a microfluidic chip can comprise a housing having walls that define a vacuum chamber and a first receptacle disposed within the vacuum chamber, the first receptacle defining a space for receiving one or more microfluidic chips. The apparatus can also include a negative pressure source, a light source, and an optical sensor coupled to the housing. The negative pressure source can be configured to reduce pressure within the vacuum chamber, the light source can be positioned to illuminate at least a portion of the space for receiving the chip(s), and the optical sensor can be positioned to capture an image of at least a portion of the space for receiving the chip(s).

公开(公告)号：US12121899B2

公开(公告)日：2024-10-22

申请号：US17261992

申请日：2019-07-24

申请人： Northeastern University

发明人： Wenjing Kang ,  Tania Konry ,  Saheli Sarkar

IPC分类号： B01L3/00 ,  G01N33/50

CPC分类号： B01L3/502784 ,  G01N33/5005 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0819

摘要： A high throughput microdroplet-based system for single cell assays in microdroplets is provided. The system integrates parallel devices and switches to enable simultaneous analysis of different cells or cell combinations, or different assay conditions, on a single microfluidic chip. Interconnections between the inlets of individual devices on a common chip enable simultaneous screening of the effect of different combinations of drugs on single cells. The use of an oil inlet and microchannels with matched total flow resistance allows the synchronous generation of droplets with the same dimensions and/or volumes.

公开(公告)号：US20240342720A1

公开(公告)日：2024-10-17

申请号：US18577431

申请日：2022-07-08

申请人： LIGHTCAST DISCOVERY LTD

发明人： Thomas Henry ISAAC ,  William Michael DEACON ,  Jasmin Kaur Chana CONTERIO

IPC分类号： B01L3/00

CPC分类号： B01L3/502784 ,  B01L3/502715 ,  B01L2300/0654 ,  B01L2400/0427

摘要： An apparatus comprising a microfluidic device comprising a microfluidic space configured to contain a plurality of microdroplets; a first light source for illuminating the microfluidic device; a voltage source for supplying a voltage to the microfluidic device in order to generate an electric field across the microfluidic space; a first modulator configured to generate a waveform signal to modulate the electric field applied across the microfluidic space, wherein the first modulator is configured to switch between an active state, in which the electric field is applied across the microfluidic space to hold the plurality of microdroplets; and a non-active state, in which the electric field is not applied across the microfluidic space; and an optical imaging device for generating an image of at least a subset of the microdroplets; wherein the first modulator is further configured to, directly or indirectly, control the first light source such that at least a portion of the light is provided across the microfluidic device during the active state; and wherein the first modulator is further configured to control the optical imaging device such that the image of at least a subset of the microdroplets is generated during the non-active state.

公开(公告)号：US20240342708A1

公开(公告)日：2024-10-17

申请号：US18610057

申请日：2024-03-19

申请人： National Research Council of Canada

发明人： Ryan Denomme ,  Lidija Malic ,  Daniel Brassard ,  Keith Morton ,  Teodor Veres

IPC分类号： B01L3/00 ,  G01N21/552 ,  G01N33/543

CPC分类号： B01L3/502715 ,  B01L3/502746 ,  B01L3/502784 ,  B01L3/502792 ,  G01N21/554 ,  G01N33/54386 ,  B01L2300/0645 ,  B01L2300/0663 ,  G01N2201/023 ,  G01N2201/061

摘要： A plasmon resonance system, instrument, cartridge, and methods for analysis of analytes is disclosed. A PR system is provided that may include a DMF-LSPR cartridge that may support both digital microfluidic (DMF) capability and localized surface plasmon resonance (LSPR) capability for analysis of analytes. In some examples, the DMF portion of the DMF-LSPR cartridge may include an electrode arrangement for performing droplet operations, whereas the LSPR portion of the DMF-LSPR cartridge may include an LSPR sensor. In other examples, the LSPR portion of the DMF-LSPR cartridge may include an in-line reference channel, wherein the in-line reference channel may be a fluid channel including at least one functionalized LSPR sensor (or sample spot) and at least one non-functionalized LSPR sensor (or reference spot). Additionally, methods of using the PR system for analysis of analytes are provided.

公开(公告)号：US12103004B2

公开(公告)日：2024-10-01

申请号：US16980358

申请日：2019-03-12

申请人： SILICON VALLEY SCIENTIFIC, INC.

发明人： Stevan Bogdan Jovanovich

IPC分类号： B01L3/00 ,  C12N15/10 ,  G01N1/28 ,  G01N35/00 ,  G01N35/08 ,  G16B5/00

CPC分类号： B01L3/502784 ,  B01L3/502761 ,  B01L3/50857 ,  C12N15/1013 ,  G01N1/286 ,  G01N35/0099 ,  G01N35/08 ,  G16B5/00 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/18

摘要： Provided herein is a system comprising: a) spatial sampler system configured to collect and transmit one or a plurality of cells or nuclei from a tissue specimen, the system comprising: i) a lower carrier having an array of conduits passing therethrough, each conduit comprising an opening on a first side and communicating with an opening on a second side, wherein the opening on the second side either (1) terminates in a capillary or (2) opens onto a well of a multiwell plate; ii) positioned, above the lower carrier, a perforated specimen holder configured to support a frozen tissue specimen, wherein the specimen holder comprises a plurality of perforations having a size sufficient to permit the passage of single cells or nuclei; and iii) positioned, above the specimen holder, a multifunctional head comprising an upper array of upper conduits, optionally, each aligned with a conduit opening of the lower carrier.

公开(公告)号：US20240316556A1

公开(公告)日：2024-09-26

申请号：US18559286

申请日：2022-05-05

申请人： BioSkryb Genomics, Inc.

发明人： Jay A.A. West

IPC分类号： B01L3/00 ,  C12Q1/6844

CPC分类号： B01L3/502784 ,  B01L3/5085 ,  C12Q1/6844 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/047 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/14 ,  B01L2400/0487 ,  B01L2400/0688

摘要： Provided herein are compositions and methods for high-throughput Primary Template-Directed Amplification (PTA) nucleic acid amplification and sequencing methods, and their applications for mutational analysis in research, diagnostics, and treatment. Further provided herein are methods for parallel analysis of DNA, RNA, and/or proteins from single cells.

公开(公告)号：US20240301517A1

公开(公告)日：2024-09-12

申请号：US18590291

申请日：2024-02-28

申请人： Versitech Limited ,  Advanced Biomedical Instrumentation Centre Limited ,  Centre for Immunology & Infection Limited

发明人： Ho Cheung SHUM ,  Lit Man POON ,  Lang NAN ,  Man Yuk Alison LAI

IPC分类号： C12Q1/70 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/6874 ,  G01N21/64

CPC分类号： C12Q1/70 ,  B01L3/502784 ,  C12N15/1096 ,  C12Q1/6874 ,  G01N21/6486 ,  B01L2200/0636 ,  B01L2300/0663 ,  B01L2300/0809 ,  B01L2400/0487

摘要： The subject invention pertains to a microfluidic pipeline which enables isolation and analysis of single viruses. Single viruses are encapsulated and manipulated within microfluidic droplets. The subject invention further pertains to the amplification of single viral genomes are amplified and confined within the droplets, followed by extraction and isolation of the single-droplet contents for sequencing analysis.

公开(公告)号：US20240299944A1

公开(公告)日：2024-09-12

申请号：US18664102

申请日：2024-05-14

申请人： Illumina, Inc.

发明人： Arash Jamshidi ,  Yan-you Lin ,  Farnaz Absalan ,  Sarah Stuart ,  Gordon Cann ,  Yir-Shyuan Wu ,  Tarun Khurana ,  Jeffrey S Fisher

IPC分类号： B01L3/00 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N1/28 ,  G01N15/10 ,  G01N33/543

CPC分类号： B01L3/502761 ,  B01L3/502784 ,  B01L3/5088 ,  C12Q1/6806 ,  G01N1/28 ,  G01N33/54333 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/165 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/185 ,  G01N2015/1006

摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

公开(公告)号：US20240293817A1

公开(公告)日：2024-09-05

申请号：US18655434

申请日：2024-05-06

申请人： Xilis, Inc.

发明人： Bradley Scott Thomas ,  Timothy A. Miller ,  David Stafford

IPC分类号： B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M3/06 ,  G01N15/14 ,  G01N15/1433 ,  G01N15/1434

CPC分类号： B01L3/502784 ,  B01L3/50273 ,  B01L3/502746 ,  C12M23/16 ,  C12M23/40 ,  C12M25/16 ,  C12M41/12 ,  C12M41/36 ,  C12M41/40 ,  G01N15/1433 ,  G01N15/1434 ,  G01N15/1459 ,  B01L2200/061 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/14 ,  B01L2300/161 ,  B01L2300/1805 ,  B01L2400/082 ,  G01N2015/1493

摘要： A method includes flowing a first fluid through a first channel of a microfluidic apparatus and flowing a second fluid through a second channel of the microfluidic apparatus. The first fluid comprises biological material and a matrix material and is immiscible with the second fluid. The first and second fluids are combined at a junction to form droplets of the first fluid dispersed in the second fluid in a third channel. Multiple exposures of a droplet in the third channel are captured in a single image, comprising: illuminating a region of the third channel with multiple successive illumination pulses during a single frame of the imaging device; identifying the droplet and determining a velocity or a size of the droplet based on an analysis of the captured exposures; and controlling the flow of the first fluid or second fluid to obtain droplets of a target size or velocity.

公开(公告)号：US20240253043A1

公开(公告)日：2024-08-01

申请号：US18290301

申请日：2022-05-22

申请人： Hunter BioDiscovery, Inc.

发明人： Tal Raz ,  Xiao Wang ,  Boryana Zhelyazkova Pursley ,  David Allan Weitz ,  Joshua Seth Wachman ,  Efstathios George Eleftheriadis ,  Anthony Robert Soltis

IPC分类号： B01L3/00

CPC分类号： B01L3/502784 ,  B01L3/5085 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/0829 ,  B01L2400/0457 ,  B01L2400/0463 ,  B01L2400/0469

摘要： A microfluidic system includes a matrix structure having a plurality of wells, each of the wells being accessible via at least one microfluidic path connectable via an interface to at least one droplet input for receiving one or more sets of droplets from one or more droplet sources, wherein a droplet enters a well based on one or more of: buoyancy, gravity, hydrodynamic force, and/or mechanical capturing, and wherein contents of a particular well are determinable based on a position of the particular well in the matrix structure and on inputs to the matrix structure. Methods using the matrix structure.