公开(公告)号：US20140302503A1

公开(公告)日：2014-10-09

申请号：US14201752

申请日：2014-03-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam Lowe ,  Geoff McDermott ,  Chris Hindson ,  Erin Chia ,  Amy Hiddessen ,  Ben Hindson ,  Chunxiao Han ,  Yaqi Wang

IPC: C12Q1/68 ,  B01L7/00

CPC classification number: C12Q1/6804 ,  B01L3/502784 ,  B01L7/52 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q2527/125 ,  C12Q2563/159 ,  C12Q2537/143

Abstract: The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

Abstract translation: 本公开提供了用于在聚合酶链反应测定中检测核酸的方法，装置，系统和组合物，例如液滴数字聚合酶链反应（ddPCR）测定。 本公开提供了使用插层染料在ddPCR测定中检测核酸的方法，装置，系统和组合物。 具有至少一种含氟表面活性剂和至少一种非离子非氟表面活性剂的双表面活性剂体系可用于液滴产生和核酸检测。

公开(公告)号：US20140179544A1

公开(公告)日：2014-06-26

申请号：US14020742

申请日：2013-09-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Erin Steenblock ,  Amy L. Hiddessen ,  Ben Hindson ,  Kevin Ness

IPC: C12Q1/68

Abstract: The present disclosure provides assays and devices for forming, spacing, and/or detecting droplets. The droplets may be emulsions composed of two or more immiscible fluids. An emulsion can be a double emulsion, such as water-in-oil droplets that are present in a continuous aqueous phase. The double emulsion can be formed when the water-in-oil droplets are contacted with one or more streams of aqueous fluid(s). This disclosure also provides a variety of additives that can be added to the fluids.

Abstract translation: 本公开提供用于形成，间隔和/或检测液滴的测定和装置。 液滴可以是由两种或更多种不混溶流体组成的乳液。 乳液可以是双重乳液，例如存在于连续水相中的油包水油滴。 当油包水滴与一种或多种含水流体物流接触时，可以形成双重乳液。 本公开还提供了可以添加到流体中的各种添加剂。