公开(公告)号：US08560247B2

公开(公告)日：2013-10-15

申请号：US13625757

申请日：2012-09-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeffrey Lerner

IPC: G01N33/48 ,  G06F11/30

CPC classification number: G06F17/10 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/165 ,  C12Q2545/10 ,  C12Q2545/114 ,  C12Q2531/113

Abstract: Systems and methods are provided for analyzing data to determine properties of a PCR process or other process exhibiting amplification or growth. Data representing an amplification can be distinguished from data representing a jump or other error. A modified sigmoid function containing a drift term may be used in determining the properties. A multi-stage functional fit of the amplification data can provide increased accuracy and consistency of one or more of the properties. A baseline of the amplification data can be determined by analyzing an integrated area of a first derivative function of the data. A reference quantitation value can also be determined from locations of maxima of different derivative functions of the amplification data, e.g., a weighted average of the maxima locations for the second and third derivatives may be used.

Abstract translation: 提供系统和方法用于分析数据以确定PCR过程的性质或显示放大或生长的其它过程。 表示放大的数据可以与表示跳跃或其他错误的数据区分开。 可以使用包含漂移项的修改后的S形函数来确定属性。 扩增数据的多阶段功能拟合可以提供一种或多种性质的增加的精度和一致性。 可以通过分析数据的一阶导数函数的积分区域来确定放大数据的基线。 参考定量值也可以从放大数据的不同导数函数的最大值的位置确定，例如可以使用第二和第三导数的最大值位置的加权平均值。

公开(公告)号：US09442891B2

公开(公告)日：2016-09-13

申请号：US14043786

申请日：2013-10-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeffrey Lerner

IPC: G01N33/48 ,  G06F17/10 ,  G06F11/30

CPC classification number: G06F17/10 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/165 ,  C12Q2545/10 ,  C12Q2545/114 ,  C12Q2531/113

Abstract: Systems and methods are provided for analyzing data to determine properties of a PCR processor other process exhibiting amplification or growth. Data representing an amplification can be distinguished from data representing a jump or other error. A modified sigmoid function containing a drift term may be used in determining the properties. A multi-stage functional fit of the amplification data can provide increased accuracy and consistency of one or more of the properties. A baseline of the amplification data can be determined by analyzing an integrated area of a first derivative function of the data. A reference quantitation value can also be determined from locations of maxima of different derivative functions of the amplification data, e.g., a weighted average of the maxima locations for the second and third derivatives may be used.

Abstract translation: 提供了系统和方法用于分析数据以确定PCR处理器表现出放大或生长的其他过程的性质。 表示放大的数据可以与表示跳跃或其他错误的数据区分开。 可以使用包含漂移项的修改后的S形函数来确定属性。 扩增数据的多阶段功能拟合可以提供一种或多种性质的增加的精度和一致性。 可以通过分析数据的一阶导数函数的积分区域来确定放大数据的基线。 参考定量值也可以从放大数据的不同导数函数的最大值的位置确定，例如可以使用第二和第三导数的最大值位置的加权平均值。

公开(公告)号：US10127350B2

公开(公告)日：2018-11-13

申请号：US14790810

申请日：2015-07-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeffrey Lerner

IPC: G06F19/22

Abstract: To determine a peak signal corresponding to a particular part of a sample, a data signal for a cluster can be transformed into a set of localized functions. For example, the data signal can be transformed to obtain coefficients of a set of wavelets, which can span a variety of scales that define an exponential decay of the wavelet. The coefficients in a time region for which a peak signal is to be obtained can be replaced with coefficients that model the behavior of tails of other peaks. The tail model can be determined using nonlinear regression, which may need to only be performed once. An inverse transform can then provide a background signal that can be subtracted from the input data signal to provide the desired peak signal.

公开(公告)号：US20130018593A1

公开(公告)日：2013-01-17

申请号：US13625757

申请日：2012-09-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeffrey Lerner

IPC: G06F19/00

CPC classification number: G06F17/10 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/165 ,  C12Q2545/10 ,  C12Q2545/114 ,  C12Q2531/113

Abstract: Systems and methods are provided for analyzing data to determine properties of a PCR process or other process exhibiting amplification or growth. Data representing an amplification can be distinguished from data representing a jump or other error. A modified sigmoid function containing a drift term may be used in determining the properties. A multi-stage functional fit of the amplification data can provide increased accuracy and consistency of one or more of the properties. A baseline of the amplification data can be determined by analyzing an integrated area of a first derivative function of the data. A reference quantitation value can also be determined from locations of maxima of different derivative functions of the amplification data, e.g., a weighted average of the maxima locations for the second and third derivatives may be used.

Abstract translation: 提供系统和方法用于分析数据以确定PCR过程的性质或显示放大或生长的其它过程。 表示放大的数据可以与表示跳跃或其他错误的数据区分开。 可以使用包含漂移项的修改后的S形函数来确定属性。 扩增数据的多阶段功能拟合可以提供一种或多种性质的增加的精度和一致性。 可以通过分析数据的一阶导数函数的积分区域来确定放大数据的基线。 参考定量值也可以从放大数据的不同导数函数的最大值的位置确定，例如可以使用第二和第三导数的最大值位置的加权平均值。

公开(公告)号：US20160004815A1

公开(公告)日：2016-01-07

申请号：US14790810

申请日：2015-07-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeffrey Lerner

IPC: G06F19/18 ,  G06F19/22

CPC classification number: G06F19/22 ,  G06F19/18 ,  G06F19/24

Abstract: To determine a peak signal corresponding to a particular part of a sample, a data signal for a cluster can be transformed into a set of localized functions. For example, the data signal can be transformed to obtain coefficients of a set of wavelets, which can span a variety of scales that define an exponential decay of the wavelet. The coefficients in a time region for which a peak signal is to be obtained can be replaced with coefficients that model the behavior of tails of other peaks. The tail model can be determined using nonlinear regression, which may need to only be performed once. An inverse transform can then provide a background signal that can be subtracted from the input data signal to provide the desired peak signal.

Abstract translation: 为了确定与样本的特定部分相对应的峰值信号，可以将簇的数据信号变换成一组局部函数。 例如，数据信号可以被变换以获得一组小波的系数，该小波可以跨越定义小波的指数衰减的各种尺度。 要获得峰值信号的时间区域中的系数可以用对其他峰值尾部行为进行建模的系数代替。 尾模型可以使用非线性回归来确定，这可能只需要执行一次。 然后，逆变换可以提供可以从输入数据信号中减去以提供期望的峰值信号的背景信号。

公开(公告)号：US20140032126A1

公开(公告)日：2014-01-30

申请号：US14043786

申请日：2013-10-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeffrey Lerner

IPC: G06F17/10

CPC classification number: G06F17/10 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/165 ,  C12Q2545/10 ,  C12Q2545/114 ,  C12Q2531/113

Abstract: Systems and methods are provided for analyzing data to determine properties of a PCR processor other process exhibiting amplification or growth. Data representing an amplification can be distinguished from data representing a jump or other error. A modified sigmoid function containing a drift term may be used in determining the properties. A multi-stage functional fit of the amplification data can provide increased accuracy and consistency of one or more of the properties. A baseline of the amplification data can be determined by analyzing an integrated area of a first derivative function of the data. A reference quantitation value can also be determined from locations of maxima of different derivative functions of the amplification data, e.g., a weighted average of the maxima locations for the second and third derivatives may be used.

Abstract translation: 提供了系统和方法用于分析数据以确定PCR处理器表现出放大或生长的其他过程的性质。 表示放大的数据可以与表示跳跃或其他错误的数据区分开。 可以使用包含漂移项的修改后的S形函数来确定属性。 扩增数据的多阶段功能拟合可以提供一种或多种性质的增加的精度和一致性。 可以通过分析数据的一阶导数函数的积分区域来确定放大数据的基线。 参考定量值也可以从放大数据的不同导数函数的最大值的位置确定，例如可以使用第二和第三导数的最大值位置的加权平均值。