公开(公告)号：US20140179544A1

公开(公告)日：2014-06-26

申请号：US14020742

申请日：2013-09-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Erin Steenblock ,  Amy L. Hiddessen ,  Ben Hindson ,  Kevin Ness

IPC: C12Q1/68

Abstract: The present disclosure provides assays and devices for forming, spacing, and/or detecting droplets. The droplets may be emulsions composed of two or more immiscible fluids. An emulsion can be a double emulsion, such as water-in-oil droplets that are present in a continuous aqueous phase. The double emulsion can be formed when the water-in-oil droplets are contacted with one or more streams of aqueous fluid(s). This disclosure also provides a variety of additives that can be added to the fluids.

Abstract translation: 本公开提供用于形成，间隔和/或检测液滴的测定和装置。 液滴可以是由两种或更多种不混溶流体组成的乳液。 乳液可以是双重乳液，例如存在于连续水相中的油包水油滴。 当油包水滴与一种或多种含水流体物流接触时，可以形成双重乳液。 本公开还提供了可以添加到流体中的各种添加剂。

公开(公告)号：US20150031034A1

公开(公告)日：2015-01-29

申请号：US14493264

申请日：2014-09-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Philip Belgrader ,  Kevin Ness ,  Michael Lucero ,  Billy Colston

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6827 ,  C12Q2537/157 ,  C12Q2563/161 ,  C12Q2533/107 ,  C12Q2521/319

Abstract: This invention provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some cases, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

Abstract translation: 本发明提供了用于检测靶多核苷酸拷贝数差异的组合物和方法。 在一些情况下，当起始样品是母体组织（例如血液，血浆）时，本文提供的方法和组合物可用于诊断胎儿遗传异常。 描述的方法和材料适用于允许检测多核苷酸拷贝数的小但统计学上显着的差异的技术。