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公开(公告)号：US11499181B2
公开(公告)日：2022-11-15
申请号：US16235996
申请日：2018-12-28
发明人： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, Jr.
IPC分类号： C12Q1/6858 , C12Q1/683 , C12Q1/6883 , C12Q1/6806 , C12Q1/6827 , C12Q1/686
摘要： Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.
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公开(公告)号：US20190241947A1
公开(公告)日：2019-08-08
申请号：US16235996
申请日：2018-12-28
发明人： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, JR.
IPC分类号： C12Q1/6858 , C12Q1/6806 , C12Q1/686 , C12Q1/6827
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q1/683 , C12Q1/6858 , C12Q1/686 , C12Q1/6883 , C12Q2535/125 , C12Q2600/158 , C12Q2600/172 , C12Q2537/16 , C12Q2563/159 , C12Q2537/165
摘要： Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.
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公开(公告)号：US10190115B2
公开(公告)日：2019-01-29
申请号：US14493272
申请日：2014-09-22
发明人： Serge Saxonov
IPC分类号： C12N15/00 , C12Q1/68 , C12N15/10 , C12Q1/6876
摘要： Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
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公开(公告)号：US20170151558A1
公开(公告)日：2017-06-01
申请号：US15351354
申请日：2016-11-14
发明人： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia , Billy W. Colston, JR. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
CPC分类号： B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08
摘要： System, including methods, apparatus, and kits, for forming emulsions. In an exemplary method of generating droplets, a device may be selected that includes a plurality of emulsion-formation units each including a sample well, a continuous-phase well, a droplet well, and a channel network that fluidically interconnects the wells and creates a droplet-generation region. A discrete volume of sample-containing fluid may be placed into the sample well of each emulsion-formation unit, and a discrete volume of continuous-phase fluid into the continuous-phase well of each emulsion-formation unit. Pressure may be applied to the device with a fluidics assembly after the step of placing, such that the plurality of emulsion-formation units generate droplets in parallel with one another. A pressure signal may be detected from the fluidics assembly. Application of the pressure may be stopped when the pressure signal indicates that a sample well is empty.
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公开(公告)号：US20220008914A1
公开(公告)日：2022-01-13
申请号：US17486667
申请日：2021-09-27
发明人： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia , Billy W. Colston, JR. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
摘要： Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
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公开(公告)号：US10760073B2
公开(公告)日：2020-09-01
申请号：US16223416
申请日：2018-12-18
发明人： Serge Saxonov
IPC分类号： C12N15/00 , C12Q1/68 , C12N15/10 , C12Q1/6876
摘要： Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
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公开(公告)号：US20170205345A9
公开(公告)日：2017-07-20
申请号：US14099750
申请日：2013-12-06
IPC分类号： G01N21/64
CPC分类号： G01N21/64 , G01N21/6428 , G01N2021/6432 , G01N2021/6439 , G01N2021/6441 , G06F19/12 , G06F19/18
摘要： System, including methods and apparatus, for performing a multiplexed digital assay.
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公开(公告)号：US20150011432A1
公开(公告)日：2015-01-08
申请号：US14493268
申请日：2014-09-22
发明人： Serge Saxonov
IPC分类号： C12N15/10
CPC分类号： C12N15/1065 , C12N15/10 , C12N15/1075 , C12Q1/6876 , C12Q2525/191 , C12Q2563/179
摘要： Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
摘要翻译： 本文提供了用于测定的方法，组合物和试剂盒，其中许多涉及扩增反应，例如数字PCR或液滴数字PCR。 测定可以用于诸如测序，拷贝数变异分析等的应用。 在一些情况下，测定涉及将样品细分成多个分隔（例如，液滴），并将分区与包括具有条形码的适配器的其它分区合并。
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公开(公告)号：US20150011430A1
公开(公告)日：2015-01-08
申请号：US14493272
申请日：2014-09-22
发明人： Serge Saxonov
IPC分类号： C12Q1/68
CPC分类号： C12N15/1065 , C12N15/10 , C12N15/1075 , C12Q1/6876 , C12Q2525/191 , C12Q2563/179
摘要： Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
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公开(公告)号：US11939573B2
公开(公告)日：2024-03-26
申请号：US16935754
申请日：2020-07-22
发明人： Serge Saxonov
IPC分类号： C12N15/10 , C12Q1/6876
CPC分类号： C12N15/1065 , C12N15/10 , C12N15/1075 , C12Q1/6876 , C12N15/10 , C12Q2525/191 , C12Q2563/179
摘要： Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
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