公开(公告)号：US10011860B2

公开(公告)日：2018-07-03

申请号：US14892340

申请日：2014-04-03

申请人： Bruker Daltonik GmbH

发明人： Christoph Lange ,  Katrin Sparbier

IPC分类号： C12Q1/04 ,  G01N33/68 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/44

CPC分类号： C12Q1/04 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/44 ,  G01N33/6851 ,  G01N2333/922 ,  G01N2333/936 ,  G01N2496/00

摘要： The invention relates to a mass-spectrometric method to determine microbial resistances to antibiotics, in which the microbes are cultured in a medium comprising an antibiotic, and a mass spectrum of the microbes is acquired after they have been cultured. The method is characterized by the fact that any microbial growth taking place during the culture is mass-spectrometrically determined with the aid of a reference substance, which is added in a dosed amount and is co-measured in the mass spectrum, wherein a growth in microbes indicates the resistance to the antibiotic.

公开(公告)号：US10913968B2

公开(公告)日：2021-02-09

申请号：US16009445

申请日：2018-06-15

申请人： Bruker Daltonik GmbH

发明人： Christoph Lange ,  Katrin Sparbier

IPC分类号： C12Q1/04 ,  G01N33/68 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/44

摘要： The invention relates to a mass-spectrometric method to determine microbial resistances to antibiotics, in which the microbes are cultured in a medium comprising an antibiotic, and a mass spectrum of the microbes is acquired after they have been cultured. The method is characterized by the fact that any microbial growth taking place during the culture is mass-spectrometrically determined with the aid of a reference substance, which is added in a dosed amount and is co-measured in the mass spectrum, wherein a growth in microbes indicates the resistance to the antibiotic.

公开(公告)号：US10457972B2

公开(公告)日：2019-10-29

申请号：US14271508

申请日：2014-05-07

申请人： Bruker Daltonik GmbH

发明人： Jochen Franzen ,  Markus Kostrzewa ,  Christoph Lange ,  Katrin Sparbier

IPC分类号： C12Q1/04

摘要： The invention relates to a mass spectrometric method for determining microbial resistances to antibiotics. The invention provides specific methods comprising cultivation in synthetic media, in which several amino acids, preferably only a single amino acid, are isotopically labeled by incorporating 13C, 15N, 18O or 34S. If several amino acids are isotopically labeled, they are labeled in such a way that they are each heavier than the corresponding unlabeled amino acids by the same integer mass difference Δm. This ensures that the mass shifts of the peaks always amount to an integer multiple of the mass difference Δm. The total mass difference can be kept relatively small by selecting suitable amino acids. A mass shift of the protein peaks in media with antibiotics indicates that the microbes are resistant. A second embodiment first produces isotopically labeled microbes, which are then tested for their resistance by cultivating them in normal media.