公开(公告)号：US11504438B2

公开(公告)日：2022-11-22

申请号：US16656417

申请日：2019-10-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Anupama Lakshmanan ,  Arash Farhadi ,  Suchita P. Nety ,  Raymond W. Bourdeau ,  Mikhail Shapiro

IPC: A61K49/22 ,  C07K14/195 ,  A61K49/00 ,  A61B8/08

Abstract: Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.

公开(公告)号：US11118210B2

公开(公告)日：2021-09-14

申请号：US15663635

申请日：2017-07-28

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Raymond W. Bourdeau ,  Anupama Lakshmanan ,  Arash Farhadi ,  Mikhail G. Shapiro ,  Audrey Lee-Gosselin

IPC: C12Q1/10 ,  A61K49/22 ,  A61K49/18 ,  C12N15/74 ,  C07K14/32 ,  C12N15/70 ,  C07K14/195

Abstract: Hybrid gas vesicle gene cluster (GVGC) configured for expression in a prokaryotic host are described comprising gas vesicle assembly (GVA) genes native to a GVA prokaryotic species and capable of being expressed in a functional form in the prokaryotic host, and one or more gas vesicle structural (GVS) genes native to one or more GVS prokaryotic species, at least one of the one or more GVS prokaryotic species different from the GVA prokaryotic species, and related gas vesicle reporting (GVR) genetic circuits, genetic, vectors, engineered cells, and related compositions methods and systems to produce GVs, hybrid GVGC and/or image a target site.

公开(公告)号：US12209268B2

公开(公告)日：2025-01-28

申请号：US17334953

申请日：2021-05-31

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Raymond W. Bourdeau ,  Anupama Lakshmanan ,  Arash Farhadi ,  Mikhail G. Shapiro ,  Audrey Lee-Gosselin

IPC: C12N15/75 ,  A61K49/18 ,  A61K49/22 ,  C07K14/195 ,  C07K14/32 ,  C12N15/70 ,  C12N15/74 ,  C12Q1/10

Abstract: Hybrid gas vesicle gene cluster (GVGC) configured for expression in a prokaryotic host are described comprising gas vesicle assembly (GVA) genes native to a GVA prokaryotic species and capable of being expressed in a functional form in the prokaryotic host, and one or more gas vesicle structural (GVS) genes native to one or more GVS prokaryotic species, at least one of the one or more GVS prokaryotic species different from the GVA prokaryotic species, and related gas vesicle reporting (GVR) genetic circuits, genetic, vectors, engineered cells, and related compositions methods and systems to produce GVs, hybrid GVGC and/or image a target site.